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전체 선택해제
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본문검색 결과만 보기
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Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick
Guimin Zhao, Hongmei Wang, Peili Hou, Chengqiang He, Hongbin He 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 10 242-250 (9 pages)
by performing agarose-gel electrophoresis. When the lateral flow probe is cut by the nfo nuclease at the dSpacer position, the probe is transformed into a primer and acts by priming the polymerase extension. Therefore, there were two bands visible in the electrophoresis results. Lateral flow dipstick assay Visualization of RPA-nfo amplicons was carried out by using LFD assays (HybriDetect;... a lateral flow dipstick (LFD) assay was developed to estimate DNA from Mycobacterium avium subsp. paratuberculosis. First, analytical specificity and sensitivity of the RPA-nfo primer and probe sets were assessed. The assay successfully detected M. paratuberculosis DNA in 30 min at 39°C with a detection limit of up to eight copies per reaction, which was equivalent to that of the real-time quantitative polymerase chain reaction (qPCR) assay. The assay was specific, as it did not amplify genomes... -
Echocardiographic assessment of coronary artery flow in normal canines and model dogs with myocardial infarction
Nohwon Park, Jaehwan Kim, Miyoung Lee, Soyun Lee, Sunhye Song, Seungjun Lee, Soyoung Kim, Yangwoo Park, Kidong Eom* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 17 149-155 (7 pages)
medicine is therefore limited. The measurement of coronary flow velocity using transthoracic Doppler echocardiography was first described in human medicine in 1987 [34]. Since then, visualization of the coronary arteries and blood flow assessment using transthoracic echocardiography has improved owing to technological advances in ultrasonography with the aid of digital imaging using a... This study was conducted to evaluate the usefulness of coronary arterial profiles from normal dogs (11 animals) and canines (six dogs) with experimental myocardial infarction (MI) induced by ligation of the left coronary artery (LCA). Blood velocity of the LCA and right coronary artery (RCA) were evaluated following transthoracic pulsed-wave Doppler echocardiography. The LCA was observed as an infundibular shape, located adjacent to the sinus of Valsalva. The RCA appeared as a tubular structure... -
Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips
Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 8 159-166 (8 pages)
thermocycler and an electrophoresis system for amplification and visualization of PCR products, respectively. The RT-RPA-LFS assay developed in this study showed no cross-reactivity with other major cereal viruses, and its sensitivity for BYDV de- tection in oat leaves was similar to that of RT-PCR. In ad- dition, the RT-RPA-LFS assay was validated with BYDV- infected field samples and aphids.... a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42oC for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus,... -
Resin Flow, Symptom Development, and Lignin Biosynthesis of Two Pine Species in Response to Wounding and Inoculation with Fusarium circinatum
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 14 394-401 (8 pages)
W. 2009. Visualization of wound periderm and hyphal profiles in pine stems inoculated with the pitch canker fungus Fusarium circinatum. Microsc. Res. Techniq. 72:965- 973. ..PAGE:8 Pine Stem Responses to Wounding and Inoculation 401 Kim, Y.-S., Woo, K.-S., Koo, Y.-B. and Yeo, J.-K. 2008b. Varia- tion in susceptibility of six pine species and hybrids to pitch canker caused by Fusarium circinatum. .... Resin flow, symptom development, and lignin biosynthesis in response to wounding and fungal inoculation were investigated in Pinus rigida and Pinus densiflora. The two-year-old seedling stems were subjected to three types of treatments: (i) wounding without inoculation, (ii) wound-inoculation with a conidial suspension of Fusarium circinatum, and (iii) pre-wounding woundinoculation with the fungus 20 days after the initial wounding. Resin flow from wounding sites was more evident in P. rigida... -
Ovary and vaginal epithelium dynamics during the estrous cycle in Dasyprocta prymnolopha Wagler, 1831
Artur N. Carreiro, Joã, o A. R. A. Diniz, Joyce G. Souza, Dé, bora V. F. Araú, jo, Rô, mulo F. F. Dias, Liliane M. S. Aze 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 14 446-451 (6 pages)
the procedures indicated for small animals, demonstrating that, despite specific anatomic characteristics, visualization in agoutis is subject to factors similar to those presented by peristalsis in domestic animals [3]. In small animals, during the proestrus phase, because of the influence of estrogen, the reproductive tract is altered, showing an increase in volume [3]. In the agouti, this... The objective of the present study was to monitor and characterize morphological alterations in ovaries of agouti (Dasyprocta prymnolopha), reared in captivity, by using abdominal ultrasonography. All animals underwent daily vaginal cytological examination to identify the current cycle phase. For each phase of the estrous cycle, ultrasound examinations were carried out to identify and describe the morphology of both ovaries. Topographic parameters in an ultrasound window were established to... -
Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice
Yu-Ying Liu, Wen-Tao Yang, Shao-Hua Shi, Ya-Jie Li, Liang Zhao, Chun-Wei Shi, Fang-Yu Zhou, Yan-Long Jiang, Jing-Tao Hu, Wei Gu, 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 5 159-167 (9 pages)
by using rabbit anti-GPV IgG as a primary antibody and HRP-conjugated labeled goat anti-rabbit IgG as a secondary antibody. An ECL Plus detection kit was used for enhanced chemiluminescence (ECL) visualization of the immunobinding [30]. Animals and ethics statement All animal experiments were accredited by the Ethics Committee (No. JLAU08201007) for Animal Care of Jilin Agriculture Universit... Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 10... -
An overview of calf diarrhea - infectious etiology, diagnosis, and intervention
Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)
not a specific test as this technique is performed to simply visualize viruses in samples and is not considered to be a sensitive procedure. In comparison, IEM has greater sensitivity than direct EM since the specimen is incubated with antibody specific for the target virus in order to agglutinate the virus before staining. The visualization of viruses, particularly ones not amenable to... Calf diarrhea is a commonly reported disease in young animals, and still a major cause of productivity and economic loss to cattle producers worldwide. In the report of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or... -
Assessment of glomerular filtration rate with dynamic computed tomography in normal Beagle dogs
Jinhwa Chang, Sujin Kim, Joohyun Jung, Heechun Lee, Hojung Choi, Dongwoo Chang, Youngwon Lee, Junghee Yoon, Mincheol Choi* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 13 393-399 (7 pages)
aorta could be also applied when performing renal angiography; by doing this, excellent visualization of the aorta and its branches is acquired [21]. Triphasic renal CT including a vascular phase is a valuable method for detecting and characterizing lesions, anatomic examination of potential kidney transplant donors, and preoperative planning for urologic surgery [6,13,32]. By performing a... The objective of our study was to determine individual and global glomerular filtration rates (GFRs) using dynamic renal computed tomography (CT) in Beagle dogs. Twenty-four healthy Beagle dogs were included in the experiment. Anesthesia was induced in all dogs by using propofol and isoflurane prior to CT examination. A single slice of the kidney was sequentially scanned after a bolus intravenous injection of contrast material (iohexol, 1 mL/kg, 300 mgI/mL). Time attenuation curves were created... -
Effect of multi-planar CT image reformatting on surgeon diagnostic performance for localizing thoracolumbar disc extrusions in dogs
Jason B. King, Jeryl C. Jones, John H. Rossmeisl Jr*, Tisha A. Harper, Otto I Lanz, Stephen R. Werre 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 8 225-232 (8 pages)
a set of volume data for interactive manipulation and visualization [5]. Reformatting software is a standard feature of most newer-generation CT scanner computers and is also available for purchase (e-Film; Merge Healthcare, USA) or via the Internet as a free download (OsiriX for Macintosh; ImageJ for Macintosh, Windows, and Linux systems). Spinal angulation can be corrected using oblique... Computed tomography (CT) is an established technique for localizing disc extrusions in dogs, however the effect of multi-planar reformatting (MPR) on surgeon diagnostic performance has not been previously described. The purpose of this study was to test the effect of MPR CT on surgeon diagnostic accuracy, certainty and agreement for localizing thoracolumbar disc extrusions in dogs. Two veterinary surgeons and one veterinary neurologist who were unaware of surgical findings independently reviewed... -
Efficacy of nano-hydroxyapatite prepared by an aqueous solution combustion technique in healing bone defects of goat
Samit Kumar Nandi, Biswanath Kundu*, Samir Kumar Ghosh, Dipak Kumar De, Debabrata Basu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 9 183-192 (10 pages)
of the arteries, one test limb from each group was collected after euthanizing the animal at the end of the experiment; the limb was perfused with lead oxide suspen- sion (20% W/V) in a manner similar to that used to examine the vascular response of host bone and surrounding tissues in the implanted area and visualization of the implant. Results Local inflammatory reactions and healing of the... The present study was undertaken to evaluate porous hydroxyapatite (HAp), the powder of which was prepared by a novel aqueous solution combustion technique, as a bone substitute in healing bone defects in vivo, as assessed by radiologic and histopathologic methods, oxytetracycline labeling, and angiogenic features in Bengal goat. Bone defects were created in the diaphysis of the radius and either not filled (group I) or filled with a HAp strut (group II). The radiologic study in group II showed...