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Molecular and genomic characterization of Mycobacterium bovis strain 1595, isolated from the laryngopharyngeal lymph node of Korean cattle
Yunho Jang, Narae Kim, Mi Hye Hwang, Hee Soo Lee, Jae Myung Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 48 108-109 (2 pages)
realizations for running the simulation algorithm, the initial information of infected farms, and divers options for control measures. Meanwhile, outputs from the simulation are daily progress of number of infected farms, daily distribution of infected farms on the actual map, and daily progress of number of infectious and culled individuals in all farms. Conclusions: This study produced the... -
Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 8 227-233 (7 pages)
strain JM109) used as host for molecular cloning and expression of plpE gene, was grown in either Luria Bertani broth (Himedia Laboratories, India) at 37 o C or Luria Bertani agar containing ampicillin at a final concentration of 50 μg/mL. Preparation of OMPs from P. multocida P52 The OMPs were extracted by the method described by Choi-Kim et al. [4]. Briefly the P. multocida serotype B: 2... Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2 and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of... -
Serological and genetic characterization of the European strain of the porcine reproductive and respiratory syndrome virus isolated in Korea
JuneYoupKim, SeungYoonLee, JungHyangSur, YoungS.Lyoo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2006, 제 46권 제 4호 9 363-370 (8 pages)
indirect fluorescence assay (IFA) Serum samples were tested for PRRS virus specific antibody responses using indirect fluorescence assay (IFA) test. IFA was carried out for initial detection of European genotype PRRSV antibody. NA and EU Fig. 2. Electrophoresis of RT-PCR for PRRS virus typing. A. Lane M: 100 bp DNA ladder Lane 1: VR2332 (NA) Lane 2: PL96-1 (NA) Lane 3: LELYSTAD (EU) Lane 4: KU-05... -
Physiological and Biochemical Changes in Lucerne (Medicago sativa) Plants Infected with ‘Candidatus Phytoplasma australasia’-Related Strain (16SrII-D Subgroup)
H?meyra Ayvacı, M. Ertu?rul G?ld?r, Murat Dikilitas 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 7 146-158 (13 pages)
yielding 1,250 bp in nested-PCR primer pairs were used. PCR products were obtained via initial denaturation (1 cycle) aW & IRU PLQ GHQaWXUaWLRQ aW & IRU PLQ SULPHU ERQGLQJ cycOHV aW & IRU PLQ HORQJaWLRQ aW & IRU PLQ ILQaO HORQJaWLRQ cycOH aW & IRU PLQ )RU WKH QHVWHG 3&5 LQLWLaO GHQaWXUaWLRQ cycOH aW & IRU PLQ GHQaWXUaWLRQ aW & IRU PLQ SULPHU ERQG- LQJ cycOHV aW & IRU PLQ HORQJaWLRQ aW & IRU... Changes in physiological and biochemical patterns in lucerne plants caused by the presence of ‘Candidatus Phytoplasma australasia’, which is one of the significant pathogens causing yield losses in lucerne plants, were investigated. Significant differences were evident in total chlorophyll, chlorophyll a, chlorophyll b, and protein amounts between ‘Ca. Phytoplasma australasia’- positive and negative lucerne plants. Stress-related metabolites such as phenol, malondialdehyde, and proline... -
Antagonistic Potential of Native Trichoderma viride Strain against Potent Tea Fungal Pathogens in North East India
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 9 278-289 (12 pages)
and Pandey, 2008). Morphological characterization and molecular identifi- cation of the antagonist. Initial the identification of Trich- oderma viride designated as strain SDRLIN1 was done on the basis of its colony and microscopic morphology (Kubicek and Harman, 2002). Further species level iden- tification was confirmed by ITS rDNA sequencing. The 550 bp fragment of ITS rDNA was amplified in... for in vitro antagonism against two important tea fungal pathogens namely Pestalotia theae and Fusarium solani. A potent antagonist against both tea pathogenic fungi, designated as SDRLIN1, was selected and identified as Trichoderma viride. The strain also showed substantial antifungal activity against five standard phytopathogenic fungi. Culture filtrate collected from stationary growth phase of the antagonist demonstrated a significantly higher degree of inhibitory activity against all the... -
Biological Efficacy of Streptomyces sp. Strain BN1 against the Cereal Head Blight Pathogen Fusarium graminearum
한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 1호 6 52-66 (15 pages)
of BN1 strain was different from those of known Streptomyces species. The phylogenetic tree showed that the ITS sequence of BN1 grouped with that of S. sampsonii with 77% BS, suggesting that BN1 does not belong to any known Streptomyces species. To classify the BN1 strain, further studies in morpho- logical characteristics, phylogenetic analyses, and serological studies are needed. The initial... small grains. Infected grains are often contaminated with mycotoxins such as zearalenone and trichothecences. During survey of contamination by FHB in rice grains, we found a bacterial isolate, designated as BN1, antagonistic to F. graminearum. The strain BN1 had branching vegetative hyphae and spores, and its aerial hyphae often had long, straight filaments bearing spores. The 16S rRNA gene of BN1 had 100% sequence identity with those found in several Streptomyces species. Phylogenetic analysis... -
Occurrence of a New Type of Pseudomonas syringae pv. actinidiae Strain of Bacterial Canker on Kiwifruit in Korea
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 4호 10 423-427 (5 pages)
280 bp band, according to the method described earlier (Rees-George et al., 2010; Vanneste et al., 2011b). These primers were designed to be complementary to a portion of the 16S-23S rDNA intertranscribed space region and are used to detect P pv. actinidiae specifically. Strains obtained from Korea and Italy were compared with P. pv. actinidiae strains previously isolated from Korea as well as the... isolated previously in Korea or the pathotype P. syringae pv. actinidiae strain. The effector hopA1 and hopH1 genes, which are known to be present in strains isolated recently from France and Italy, were also present in P. syringae pv. actinidiae strains, SYS1, SYS2 and SYS4, isolated from Korea in this work. However, no amplicons of the expected size were obtained from strains previously isolated from Korea and Japan. In addition, the Korean strains isolated in this work belonged to haplotype I... -
Identification of a Gene Encoding Adenylate Kinase Involved in Antifungal Activity Expression of the Biocontrol Strain Burkholderia pyrrocinia CH-67
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 4호 4 373-380 (8 pages)
in initial bacterial growth. This may be due to the ATP/ADP/AMP imbalance in the adenylate kinase mutant. However, the wild-type and M208 cell numbers were not different after 48-h incubation, indicating that the antifungal activity deficiency in M208 was not due to delayed bacterial growth. The M208 mutant did not exhibit antifungal activity even after 72-h incubation. Addi- tion of ADP or high... activity against several plant pathogenic fungi. Transposon mutagenesis was performed to identify the genes responsible for the antifungal activity of B. pyrrocinia CH-67. Of the 2,500 mutants tested using the Fulvia fulva spore screening method, a mutant deficient in antifungal activity, M208, was selected. DNA sequence analysis of the transposon-inserted region revealed that a gene encoding an adenylate kinase-related kinase was disrupted in M208. Antifungal activity was restored in M208 when... -
Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)
of an RE analysis- based method proved a useful epidemiological tool for rapid genotyping and identification of mixed infections by RSV strain and by different subtype. Keywords:genotyping, genetic structure, phylogenetic relationships, restriction enzyme analysis, Rice stripe virus The genome of Rice stripe virus (RSV), the prototype member of the genus Tenuivirus, consists of four ssRNAs, with a... small brown planthopper from Eastern China caught by aerial net traps that possesses RSVRNA3 genotypes corresponds mainly to Eastern China, with a few for Southwestern China based on RT-PCR/ RE, sequence and phylogenetic analyses, indicating that RSV populations in Eastern China may also have strong RNA variation. The development of an RE analysisbased method proved a useful epidemiological tool for rapid genotyping and identification of mixed infections by RSV strain and by different subtype.