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Isolation and In Vitro Culture of Vascular Endothelial Cells from Mice
ShinkyuChoi, JiAeeKim, KwanChangKim, SukHyoSuh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.1 6 35-42 (8 pages)
In vitro culture, Mouse vessels INTRODUCTION Endothelial cells (EC), which line the inner surfaces of blood vessels, not only act as a barrier between the plasma and vessel wall, but also play critical roles in the regulation of angiogenesis, blood vessel permeability, and contractility of blood vessels in response to physiologic and pathologic stimuli [1]. Therefore, EC dysfunction may... In cardiovascular disorders, understanding of endothelial cell (EC) function is essential to elucidate the disease mechanism. Although the mouse model has many advantages for in vivo and in vitro research, efficient procedures for the isolation and propagation of primary mouse EC have been problematic. We describe a high yield process for isolation and in vitro culture of primary EC from mouse arteries (aorta, braches of superior mesenteric artery, and cerebral arteries from the circle of...