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전체 선택해제
총 310건 검색
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하이브리도마 방법에 의한 항-${alpha}_1$-Proteinase Inhibitor 모노클론항체의 생산과 분석
강신성, 방옥선, 강희갑, 손종경, Kang. Shin-Sung, Bang. Ok-Sun, Kang. Hee-Kap, Sonn. Jong-Kyung 생화학분자생물학회 한국생화학회지 6 Pages
생화학분자생물학회 한국생화학회지 1988, Vol.21 No.4 491-496 (6 pages)
Alpha-1-proteinase inhibitor(${alpha}_1-PI$)로 면역시킨 Balb/c 마우스 비장세포와 SP 2/0-Ag 14 마우스 미에로마 세포를 융합시키는 하이브리도마 기법으로 모노클론 항체 생산을 실시하였다. 하이브리드클론들이 생산하는 항체들과 ${alpha}_1-PI$와의 반응성은 ELISA로 분석하였으며, 양성클론을 제한희석법으로 클로닝하여 7클론의 항-${alpha}_1-PI$ 특이 클론세포를 얻었고, 이들의 단일클론성을 확인하였다. 7클론 중 5클론이 생산하는 모노클론항체는 항원에 대한 결합상수가 $0.1-1.1{ imes}10^9M^{-1}$의 $IgG_1(k$) 이었고,... -
Carbohydrate Utilization Affects Lactobacillus delbrueckii subsp. lactis 313 Cell-enveloped-associated Proteinase Production
Agyei. Dominic, Danquah. Michael K. 한국생물공학회 Biotechnology and bioprocess engineering 8 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2012, Vol.17 No.4 787-794 (8 pages)
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Inhibitory Substance Produced by Aspergillus sp. on the Snake Venom Proteinase - Isolation of Microorganism and Biological Activities of the Inhibitor -
Hyun. Nam-Joo, Seu. Jung-Hwn 한국미생물생명공학회 산업미생물학회지 6 Pages
한국미생물생명공학회 산업미생물학회지 1987, Vol.15 No.2 129-134 (6 pages)
배양액에서 저해물질을 분리하여 Venom proteinase에 대한 작용양상과 안정성에 대한 조사결과는 다음과 같다. Glucose 2%, NaNO$_3$ 0.3%, $K_2$HPO$_4$ 0.02%, MgSO$_4$ㆍ7$H_2O$ 0.02%, KCl 0.02% 조성의 배지(pH 5.0)를 사용하여 3$0^{circ}C$에서 7일간 배양하여 얻은 배양액을 acetone 심전 활성탄, methanol 침전으로 무정형의 유효분말을 얻었다. 이 물질은 A. b.b. venom proteinase에 대하여 1/2 배양에서 약 70% 저해율을 나타냈으며, A.b.b. venom proteinase에 대한 저해양상을 혼합형이었으며 enzyme-inhibitor complex를... -
복령의 Carboxyl Proteinase의 분리 정제 및 그 성질에 관한 연구(II)
민태진, 박상신, 문순구, Min. Tae-Jin, Park. Sang-Shin, Moon. Soon-Ku 한국균학회 한국균학회지 7 Pages
한국균학회 한국균학회지 1986, Vol.14 No.2 101-107 (7 pages)
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The effects of therapeutic ultrasound stimulation on the inflammation cytokine in rat articular chondrocytes
Eun-Jung Kim, Su-jin Hwang, Gye-Yeop Kim 물리치료재활과학회 Physical Therapy Rehabilitation Science 6 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2013, 제 2권 제 1호 4 21-26 (6 pages)
stage [9]. The histological pathology re- flects the result of joint inflammation and cartilage erosion. Since the initial stages of OA involve increased proteinase, inflammation cytokines, and other inflammatory mediators by chondrocytes [10]. OA related between the increased concentration of catabolic enzymes and inflammatory medi- ators such as cyclo-oxygenase 2, nitric oxide, interleukin 1... Objective: The aim of this study was to investigate the effect of therapeutic ultrasound (US) of cell viability and inflammatory cytokine in rat articular chondrocyte cultures stimulated with lipopolysaccharide (LPS). Design: One group pretest-posttest design. Methods: Cultured chondrocytes were treated with US and/or LPS and assessed for viability, Tumor necrosis factor (TNF)-α and Interleukin (IL)-1 production. Results: Oxidative stress was induced in rat chondrocytes with LPS. The cell... -
The Transmission of Chronic Wasting Disease in Transgenic mice over-expressing Goat prion protein
Hoo Chang Park, Hyun Joo Sohn, Kyung Jae Park, In Soon Roh, Hyo Jin Kim, Yoon Hee Lee, In Soo Cho, Byoung han Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 180 430-431 (2 pages)
they were intracerebrally inoculated into transgenic elk prion overexpressing mice. -Western Blotting for PrP Sc detection: The samples (20 uL) after each round of amplification were mixed with proteinase K (200 ug/ml) and incubated 37 ℃ for 1hr. Samples were separated by SDS-PAGE and transferred onto PVDF membrane (Milipore, USA). After blocking, the membranewas incubated for 1hr with 1st... -
Protein Misfolding Cyclic Amplification of CWD Prion Protein in Soil
Kyung Je Park, Hyun Joo Sohn, In Soon Roh, Hyo Jin Kim, Byoung han Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 179 429-430 (2 pages)
they were intracerebrally inoculated into transgenic elk prion overexpressing mice. -Western Blotting for PrP Sc detection: The samples (20 uL) after each round of amplification were mixed with proteinase K (200 ug/ml) and incubated 37 ℃ for 1hr. Samples were separated by SDS-PAGE and transferred onto PVDF membrane (Milipore, USA). After blocking, the membranewas incubated for 1hr with 1st... -
Toxicoinfectious Botulism in Korean Native Chickensby Leftover Food
Gyoung Ho Moon, Il Jang, You Chan Bae 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 177 428-429 (2 pages)
consisted of 56 cycles of 30 sec of sonication followed 10 min of 37 ℃ incubation. - WesternBlotting for PrP Sc detection: The samples (20 uL) after each round of amplification were mixed with proteinase K (200 ug/mL) and incubated 37 ℃ for 1hr. Samples were separated by SDS-PAGE and transferred onto PVDF membrane (Milipore, USA). After blocking, the membranewas incubated for 1hr with 1st... -
Efficient Decontamination of PrPSc in Soil by Sodium Hypochlorite
Hyun Joo Sohn, Kyung Je Park, In Soon Roh, Hyo Jin Kim, Byoung han Kim 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 176 428-428 (1 pages)
consisted of 56 cycles of 30 sec of sonication followed 10 min of 37 ℃ incubation. - WesternBlotting for PrP Sc detection: The samples (20 uL) after each round of amplification were mixed with proteinase K (200 ug/mL) and incubated 37 ℃ for 1hr. Samples were separated by SDS-PAGE and transferred onto PVDF membrane (Milipore, USA). After blocking, the membranewas incubated for 1hr with 1st...