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Experimental infection of SPF, Korean native chickens, broiler chicken with highly pathogenic H5N6 avian influenza virus
Seok Chan Park, Byung Min Song, Eun Kyoung Lee, Gyeong Beom Heo, Yu Na Lee, Youn Jeong Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 122 272-273 (2 pages)
of ES2 virus among chicken breeds, we experimentally infected SPF chickens, broiler chicken and Korean native chickens (KNC) with ES2 virus. Materials and Methods: Three kinds of chicken breeds including SPF white Leghorn (SPF), broiler and Korean native chicken (brown line, abbreviated as KNC) were used for this study. Intravenous pathogenicity (IVPI) test with 6-week-old SPF chicken was... -
Pathogenicity of an avian paramyxovirus-4 after serial passages in chicken eggs
Su Hwan Kho, Haan Woo Sung, Hyuk Moo Kwon, Min Jun Jang, Kang Seuk Choi, Hyun Jeong Lee 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 95 236-236 (1 pages)
a wild bird-origin APMV-4 isolate through chicken embryos and then assessed the pathogenicity. Materials and Methods: APMV-4/mallard/KR/KNU23/2011, which is an APMV-4 virus isolated from mallard, was passaged 50 times in 9-day-old embryonated specific pathogen free (SPF) chicken eggs. The full fusion (F) gene and hemagglutinin-neuraminidase (HN) gene of the viruses which was passaged 5 times... -
Improvedhenhouse condition and chicken health by a regenerative far-infrared heatingsystem
Chung Young Lee, Hee Soo Kim, Jin Mi Park, Jeon Deok Kim, Choon Sik Jeon, Jae Hong Kim, Hyuk Joon Kwon 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 329 388-388 (1 pages)
of Veterinary Science P-276 Improvedhenhouse condition and chicken health by a regenerative far-infrared heatingsystem Chung-Young Lee 1, Hee-Soo Kim 1, Jin-Mi Park 1, Jeon-Deok Kim 3, Choon-Sik Jeon 3, Jae-Hong Kim 1, Hyuk-Joon Kwon *2 1Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University; 2Department of Farm Animal Medicine, College of Veterinary Medicine,... -
Marek’s disease vaccine activates chicken macrophages
Dan Wang, Shuhong Sun, Mohammad Heidari 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 7 375-383 (9 pages)
MD-susceptible and MD-resistant chicken lines. Materials and Methods Experimental chickens The specific-pathogen-free (SPF) chickens in the current study were from progenitor line 6 3 (MD-resistant) and 72 (MD-susceptible) birds [2]. These birds were from unvaccinated breeder hens and lacked antibodies to many avian pathogens including MDV, herpesvirus of turkey, reticuloendotheliosis virus... immune responses in vaccine-mediated protection, we investigated the effect of Marek’s disease (MD) vaccine, CVI988/Rispens, on the expression patterns of selected genes associated with activation of macrophages in MD-resistant and MD-susceptible chicken lines. Upregulation of interferon , interleukin (IL)-1, IL-8, and IL-12 at different days post-inoculation (dpi) revealed activation of macrophages in both chicken lines. A strong immune response was induced in cecal tonsils of... -
Chicken RNA-binding protein T-cell internal antigen-1 contributes to stress granule formation in chicken cells and tissues
Yingjie Sun, Pin Zhang, Hang Zheng, Luna Dong, Lei Tan, Cuiping Song, Xusheng Qiu, Ying Liao, Chunchun Meng, Shengqing Yu, Chan 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 2 3-12 (10 pages)
(A11055), Alexa Fluor 594-labeled goat anti-rabbit (A11012), and goat anti-mouse (A-11005) were purchased from Thermo Fisher Scientific (USA). Cells and viruses HeLa and DF-1 chicken fibroblast cells were purchased from American Type Culture Collection (ATCC; USA). Chicken embryo fibroblast (CEF) primary cells were prepared from 9-day-old specific-pathogen-free (SPF) embryonated chicken eggs... species. However, SG formation in chicken cells and the role of chicken TIA-1 (cTIA-1) in SG assembly has not been elucidated. In the present study, we cloned cTIA-1 and showed that it facilitates the assembly of canonical SGs in both human and chicken cells. Overexpression of the chicken prion-related domain (cPRD) of cTIA-1 that bore an N-terminal green fluorescent protein (GFP) tag (pntGFP-cPRD) or Flag tag (pFlag-cPRD) induced the production of typical SGs. However, C-terminal GFP-tagged... -
Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus
Yi-Yang Lien, Chi-Hung Huang, Fang-Chun Sun, Shyang-Chwen Sheu, Tsung-Chi Lu, Meng-Shiunn Lee, Shu-Chin Hsueh, Hsi-Jien Chen, Me 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 11 73-79 (7 pages)
the future. Keywords: chicken anemia virus, immunoaffinity column, immunohistochemistry, monoclonal antibody, VP1 Introduction Chicken anemia virus (CAV) is the sole member of the genus Gyrovirus of the Circoviridae family and causes severe anemia and chicken anemia disease, an immu- nosuppressive disorder [3,13,14]. Histopathological studies have shown that CAV infection leads to aplasia of... Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After... -
Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-g
Jeong Ho Park, Haan Woo Sung, Byung Il Yoon, Hyuk Moo Kwon* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 6 131-140 (10 pages)
Veterinary Science J. Vet. Sci. (2009), 10(2), 131139 DOI: 10.4142/jvs.2009.10.2.131 *Corresponding author Tel: +82-33-250-8652; Fax: +82-33-244-2367 E-mail: kwonhm@kangwon.ac.kr Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-γ Jeong Ho... tested in conjunction with the vaccine. A plasmid DNA vaccine (pcDNA-VP243) encoding the VP2, VP4, and VP3 proteins of the very virulent IBDV (vvIBDV) SH/92 strain was injected into the amniotic sac alone or in combination with a plasmid encoding chicken IL-2 (ChIL-2) or chicken IFN-g (ChIFN-g) at embryonation day 18, followed by an intramuscular injection of a commercial killed IBD vaccine at 1 week of age. The chickens were orally challenged with the vvIBDV SH/92 strain at 3 weeks of age and... -
Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease
Burak Kuscu, Aydin Gümrel* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 3 15-23 (9 pages)
chicks with experimentally induced chicken infectious anemia disease Burak Kuscu, Aydin Gurel* Department of Pathology, Faculty of Veterinary Medicine, Istanbul University, Avcilar 34310, Istanbul, Turkey One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations... One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations in the thymus and bone marrow, were observed. Tissue sections of thymus and bone marrow were stained using the streptavidin-biotin peroxidase method and examined under light microscope for evaluation of antigenic intensities in tissues. Those findings were then compared with blood parameters and ELISA... -
Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens
Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 10 361-368 (8 pages)
of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon* School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxy- nucleotides... This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxynucleotides (CpG-ODN), and chicken interferon-γ (ChIFN-γ) on a DNA vaccine (pcDNA-VP243) against the infectious bursal disease virus (IBDV). A plasmid encoding chicken IFN-a was constructed. Twice at 2-week intervals, twoweek- old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2... -
박테리오파아지 CJø07의 Salmonella enteritidis 감염에 대한 SPF 병아리에서의 효능 평가
임태현, 이현정, 김명섭, 김병윤, 양시용, 송창선, Lim. Tae-Hyun, Lee. Hyun-Jeong, Kim. Myeong-Seob, Kim. Byoung-Yoon, Yang. Si-Yong, Song. Chang-Seon 한국가금학회 한국가금학회지 5 Pages
한국가금학회 한국가금학회지 2010, Vol.37 No.3 283-287 (5 pages)
따른 장내 균증식과 체외 균배출에 미치는 영향을 평가하고자 실시하였다. 박테리오파아지를 투여한 SPF 병아리에 SE균을 공격 접종 한 뒤, 공격 접종 10일 후 채취한 맹장 분변에 존재하는 SE균 수를 측정한 결과, 박테리오파아지를 투여한 병아리의 맹장 분변 내 SE균 수는 양성 대조군에 비하여 유의성 있게 감소하였다(P<0.05). 또한, 공격 접종 10일 후 사육 시설 표면의 SE균 오염도를 측정한 결과, 박테리오파아지를 투여한 그룹의 사육 시설에서 채취한 환경 시료의 SE균 오염율(50%)이 박테리오파아지를 투여하지 않은 그룹의...