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pH-dependent modulation of intracellular free magnesium ions with ion- selective electrodes in papillary muscle of guinea pig
Shang-Jin Kim, In-Gook Cho, Hyung-Sub Kang, Jin-Shang Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 5 31-36 (6 pages)
Na +-selective microelectrodes and 100 mM MgCl2 for the Mg 2+-selective microelectrodes, and then beveled to increase the tip diameter to ~1 mm. The microelectrodes were filled with the Na + neutral carrier, ETH 227, and the Mg 2+ neutral carrier, ETH 5214, respectively. Calibration was carried out with a fixed ionic background to mimic intracellular conditions and thereby to minimize effects of... the determination of the role of pH in the cardiac magnesium homeostasis, the intracellular Mg 2+ concentration ([Mg 2+]i), membrane potential and contraction in the papillary muscle of guinea pigs using ion-selective electrodes changing extracellular pH ([pH]o)or intracellular pH ([pH ]i )were measured in this study. A high CO2-induced low [pH]o causes a significant increase in the [Mg2+]i and [Na+]i , which was accompanied by a decrease in the membrane potential and twitch force.The high [pH o... -
Calcium homeostasis in diabetes mellitus
Changhwan Ahn, Ji-Houn Kang, Eui-Bae Jeung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 1 261-266 (6 pages)
[45,52]. The rapid increase in intracellular calcium ([Ca 2+]i) triggers insulin release. The role of 1,25(OH)2D3 in insulin secretion derives from its effects on Ca 2+ influx, mobilization, and buffering in pancreatic [39]. 1,25(OH)2D3 induces rapid (within 510 sec), synchronous, sinusoidal [Ca 2+]i, and oscillations in pancreatic -cells, effects ..PAGE:2 262 Changhwan Ahn et al. Journal of... Diabetes mellitus (DM) is becoming a lifestyle-related pandemic disease. Diabetic patients frequently develop electrolyte disorders, especially diabetic ketoacidosis or nonketotic hyperglycemic hyperosmolar syndrome. Such patients show characteristic potassium, magnesium, phosphate, and calcium depletion. In this review, we discuss a homeostatic mechanism that links calcium and DM. We also provide a synthesis of the evidence in favor or against this linking mechanism by presenting recent... -
Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues
Bonn Lee, Hee Young Kang, Dong Oh Lee, Changhwan Ahn, Eui-Bae Jeung 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 2 445-451 (7 pages)
Muto S, Hata M, Taniguchi J, Tsuruoka S, Moriwaki K, Saitou M, Furuse K, Sasaki H, Fujimura A, Imai M, Kusano E, Tsukita S, Furuse M. Claudin-2-deficient mice are defective in the leaky and cation-selective paracellular permeability properties of renal proximal tubules. Proc Natl Acad Sci U S A 2010, 107, 8011-8016. 23. Nitta T, Hata M, Gotoh S, Seo Y, Sasaki H, Hashimoto N, Furuse M, Tsukit... Claudins, which are known as transmembrane proteins play an essential role in tight junctions (TJs) to form physical barriers and regulate paracellular transportation. To understand equine diseases, it is helpful to measure the tissue-specific expression of TJs in horses. Major equine diseases such as colic and West Nile cause damage to TJs. In this study, the expression level and distribution of claudin-1, -2, -4, and -5 in eight tissues were assessed by Western blotting and... -
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca2+ Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat
JeongHoonLee, JeongMiHa, ChaeHunLeem 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.4 10 373-382 (10 pages)
of those particles may increase mitochondrial buffering capacity to limit [Ca 2+]m increase. The Pi effect on mitochondrial Ca 2+ dynamics re- quires further study. In conclusion, we successfully developed a method to solve NADH and Fura-2-FF interference. This method enables more accurate Ca 2+ measurements. In particular, quantita- tive measurement of mitochondrial Ca 2+ dynamics is now... Fura-2 analogs are ratiometric fluoroprobes that are widely used for the quantitative measurement of [Ca2+]. However, the dye usage is intrinsically limited, as the dyes require ultraviolet (UV) excitation, which can also generate great interference, mainly from nicotinamide adenine dinucleotide (NADH) autofluorescence. Specifically, this limitation causes serious problems for the quantitative measurement of mitochondrial [Ca2+], as no available ratiometric dyes are excited in the visible range.... -
Potassium Currents in Isolated Deiters' Cells of Guinea Pig
JongWooChung, EuiCholNam, WonTaeKim, JaeBoumYoum, ChaeHunLeem 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.6 10 537-546 (10 pages)
+ selective channels may exist in Deiters’ cells. Using Charybdotoxin and TEA, Szucs et al. [11] isolated two distinct potassium channels that are different from Kv1.5 type K + channel in Dieters’ cells. Deiters' cells are immersed in the perilymph except the part of phalangeal proces s, which is facing the endo- lymph. Because of this difference, a big potassium gradient may be generated... Deiters' cells are the supporting cells in organ of Corti and are suggested to play an important role in biochemical and mechanical modulation of outer hair cells. We successfully isolated functionally different K+ currents from Deiters’ cells of guinea pig using whole cell patch clamp technique. With high K+ pipette solution, depolarizing step pulses activated strongly outward rectifying currents which were dose-dependently blocked by clofilium, a class III anti-arrhythmic K+ channel blocker.... -
Contradictory Effects of Superoxide and Hydrogen Peroxide on KCa3.1 in Human Endothelial Cells
ShinkyuChoi, Hye-YoungNa, JiAeeKim, Sung-EunCho, SukHyoSuh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.3 1 181-187 (7 pages)
4 Na2ATP, 10 HEPES, pH ad- justed to 7.2 with KOH. For buffering free Ca 2＋, the appro- priate amount of Ca 2＋ (calculated using CaBuf software; G. Droogmans, Leuven, Belgium; ftp://ftp.cc.kuleuven.ac.be/ pub/droogmans/cabuf.zip) was added in the presence of 5 mM EGTA. ..PAGE:3 Contradictory Effects of Different ROS on KCa3.1 Expression 183 Fig. 1. KCa3.1 expression in ROS donor-treated... Reactive oxygen species (ROS) are generated in various cells, including vascular smooth muscle and endothelial cells, and regulate ion channel functions. KCa3.1 plays an important role in endothelial functions. However, the effects of superoxide and hydrogen peroxide radicals on the expression of this ion channel in the endothelium remain unclear. In this study, we examined the effects of ROS donors on KCa3.1 expression and the K＋ current in primary cultured human umbilical vein endothelial... -
A Computational Model of Cytosolic and Mitochondrial [Ca2＋] in Paced Rat Ventricular Myocytes
JaeBoumYoum, SeongWooChoi, ChangHanJang, HyoungKyuKim, ChaeHunLeem, NariKim, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 23 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.4 5 217-239 (23 pages)
to cations such as Na ＋ , K ＋ , and Ca 2＋ . It po- tentially contributes to slight depolarization of myocytes against the equilibrium potential of K ＋ (EK). The for- mulation of non-selective cation channel is based on the model of rat heart [34] which incorporated the selective per- meability of channels to various cations. The maximum con- ductance was adjusted to reproduce the appropriate... We carried out a series of experiment demonstrating the role of mitochondria in the cytosolic and mitochondrial Ca2＋ transients and compared the results with those from computer simulation. In rat ventricular myocytes, increasing the rate of stimulation (1∼3 Hz) made both the diastolic and systolic [Ca2＋] bigger in mitochondria as well as in cytosol. As L-type Ca2＋ channel has key influence on the amplitude of Ca2＋-induced Ca2＋ release, the relation between stimulus frequency and the... -
Effects of PCB Congeners in Rodent Neuronal Cells in Culture
Sun-YoungKimJae-HoYang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.1 2 9-16 (8 pages)
2+ buffering system and caus ed protein kinase C (PKC) translocation at low micromolar concentrations, while non- ortho, coplanar PCB did not have any effects on these second messenger systems (Kodavanti et al, 1993; Koda- vanti et al, 1994; Kodavanti et al, 1996). Perturbation of Ca 2+ homeostasis leading to the sustained elevation of calcium ions can trigger many second messenger systems,... We attempted to analyze the mechanism of polychlorinated biphenyl (PCB)-induced neurotoxicity and identify the target molecules in the neuronal cells for PCBs.Since the developing neuron is particularly sensitive to PCB-induced neurotoxicity, we isolated cerebellar granule cells derived from 7-day old Sprague Dawley (SD) rats and grew cells in culture for additional 7 days to mimic PND-14 conditions. Only non-coplanar PCBs at a high dose showed a significant increase of total protein kinase C... -
Heterogeneity of the SR-dependent Inward Na+-Ca2+ Exchange Current in the Heavily Ca2+-buffered Rat Ventricular Myocytes
Kyung-BongYoon, Sung-WanAhn, ChangMannKo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.2 6 101-110 (10 pages)
-Ca 2+ exchange after restriction of the interactions between Ca 2+ from SR and Na +-Ca 2+ exchange within micro-domains with heavy cytosolic Ca 2+ buffering with 10 mM BAPTA. During stim ulation every 10 s with a pulse roughly mimicking action potential, the initial outward curr ent gradually turned into a huge inward current of -12.9±0.5 pA/pF. From the inwa rd current, two different inward... SR and Na-Ca2 exchange within micro-domains with heavy cytosolic Ca2 buffering with 10 mM BAPTA. During stimulation every 10 s with a pulse roughly mimicking action potential, the initial outward current gradually turned into a huge inward current of 12.9⁑0.5 pA/pF. From the inward current, two different inward INCXs were identified. One was 10 μM ryanodine-sensitive, constituting 14.2⁑2.3%. It was completely blocked by CdCl2 (0.1 mM and 0.5 mM) and by... -
Involvement of Oxidative Stress and Poly(ADP-ribose) Polymerase Activation in 3-Nitropropionic Acid-induced Cytotoxicity in Human Neuroblastoma Cells
EunjooNam, YoungJaeLee, YoungAhOh, JinAhJung, HyeInIm, SeongEunKoh, SunghoMaeng, WanSeokJoo, YongSikKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.6 6 325-331 (7 pages)
a buffering threshold of the cells at these concentrations. In addition, the accu- mulation of ROS was detected 4 h after 3-NP exposure. Therefore, the cells must have enough endogenous antioxi- dants to detoxify ROS accumula ted within 4 h by up to 0.6 mM 3-NP. The buffering capacity of SK-N-BE(2)C cells was further evidenced by the delaye d onset of PARP activation. The onset of PARP... 3-Nitropropionic acid (3-NP) inhibits electron transport in mitochondria, leading to a metabolic failure. In order to elucidate the mechanism underlying this toxicity, we examined a few biochemical changes possibly involved in the process, such as metabolic inhibition, generation of reactive oxygen species (ROS), DNA strand breakage, and activation of Poly(ADP-ribose) polymerase (PARP). Exposure of SK-N-BE(2)C neuroblastoma cells to 3-NP for 48 h caused actual cell death, while inhibition of...