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Translocational changes of localization of synapsin in axonal sprouts of regenerating rat sciatic nerves after ligation crush injury
Ku-birm Kwon, Jin-suk Kim, Byung-joon Chang 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 1 1-9 (9 pages)
1(1), 19 Translocational changes of localization of synapsin in axonal sprouts of regenerating rat sciatic nerves after ligation crush injury Ku-birm Kwon, Jin-suk Kim 1, Byung-joon Chang* Department of Anatomy and Histology, 1 Department of Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea Time-dependent translocational changes of Synapsin I... Time-dependent translocational changes of Synapsin I (SyI), a synaptic vesicle-associated phosphoprotein and its involvement in the axonal transport were investigated in the regenerating axonal sprouts. A weak SyI immunoreactivity (IR) was found in the axoplasm of normal axons. Rat sciatic nerves were crush-injured by ligating with 1-0 silk thread at the mid-thigh level and released from the ligation 24 h later. At various times after release, immunocytochemistry was performed. SyI was... -
Increase of Synapsin I, Phosphosynapsin (ser-9), and GAP-43 in the Rat Hippocampus after Middle Cerebral Artery Occlusion
YeonJooJung, PilWooHuh, SuJinPark, JungSunPark, KyungEunLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.2 2 77-82 (6 pages)
to regulate the plastic synaptic modifications, the synaptic vesicular proteins play an important role. Among the synaptic proteins, synapsin I is specifically associated with the cytoplasmic surface of the synaptic vesicle membrane and regulates the release of neurotrans- mitters, neurite outgrowth, and new synapse formation during normal development, following various brain lesions such as... a brain lesion. Using synapsin I and GAP-43 as markers, we investigated the neuronal cell death and the synaptic plastic modification in the rat hippocampus of a middle cerebral artery occlusion (MCAO) model. Cresyl violet staining revealed that neuronal cell damage occurred after 2 h of MCAO, which progressed during reperfusion for 2 weeks. The immunoreactivity of synapsin I and GAP-43 was increased in the stratum lucidum in the CA3 subfield as well as in the inner and outer molecular layers of... -
Glucose/Oxygen Deprivation Induces Release of [3H]5-hydroxytryptamine Associated with Synapsin 1 Expression in Rat Hippocampal Slices
EunMiPark, SangHuiChu, KyungEunLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.5 2 347-353 (7 pages)
of synapsin 1 during GOD for 20 minutes， which was also in part prevented by APV. In contrast， the expression of syntaxin and SNAP-25 were not altered during GOD. These results suggest that ischemic insult induces release of eH]5-HT associated with synapsin 1， synaptic vesicular protein， via activation of NMDA receptor in part. Key Words: Hippocampal slices， Glucosejoxygen deprivation，... of nerve cells to the ischemic neuronal injury. But there is no information regarding whether the release of monoamines is directly associated with synaptic vesicular proteins under ischemia. In the present study, it was investigated whether synapsin 1, syntaxin and SNAP-25 are involved in the release of 5-hydroxytryptamine ([3H]5-HT) in glucose/oxygen deprived (GOD) rat hippocampal slices. And, the effect of NMDA receptor using DL-2-amino-5-phosphonovaleric acid (APV) on ischemia- induced... -
사회적으로 고립된 쥐의 해마에서 NGF와 Synapsin I, ChAT의 단백질 수준에 미치는 사전운동효과
홍영표, 김현태, Hong. Young-Pyo, Kim. Hyun-Tae 한국생명과학회 생명과학회지 7 Pages
한국생명과학회 생명과학회지 2012, Vol.22 No.9 1180-1186 (7 pages)
8주)을 적용하였으며, 이후 각각 사회적 고립(Isolation, 8주)을 적용하여 분석하였다(Group/Con: GC, Group/Ex: GE, Isolation/Con: IC, Isolation/Ex: IE, 각 집단별 n=8). 실험결과, IC집단에서 GC집단에 비해 해마에서 NGF, Synapsin I 및 ChAT가 유의하게 감소한 것으로 나타났다. 반면 IE집단에서 IC집단에 비해 NGF, Synapsin I 및 ChAT의 감소가 유의하게 개선된 것으로 나타났다. 이상의 결과 사회적 고립에 의한 해마에서의 NGF, Synapsin I 및 ChAT 단백질 감소는 EPC에 의해 개선되며, 이를 통해 해마의 기능 저하를 일부... -
The Pathophysiologic Roles of TRPM7 Channel
HyunSooPark, ChansikHong, ByungJooKim, InsukSo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.1 2 15-23 (9 pages)
inergic synaptic vesicles, TRPM7 was found to be involved in transmitter release [54]. In sympathetic neurons, TRPM7 is localized in synaptic vesicles, forms complexes with syn- aptic vesicular synapsin 1 and synaptotagmin 1, and then interacts with synaptic vesicular snapin [54]. Furthermore, changes in vesicular TRPM7 ch annel activities were found to alter acetylcholine release [54]. In a... Transient receptor potential melastatin 7 (TRPM7) is a member of the melastatin-related subfamily and contains a channel and a kinase domain. TRPM7 is known to be associated with cell proliferation, survival, and development. It is ubiquitously expressed, highly permeable to Mg2＋ and Ca2＋, and its channel activity is negatively regulated by free Mg2＋ and Mg-complexed nucleotides. Recent studies have investigated the relationships between TRPM7 and a number of diseases. TRPM7 regulates cell... -
Oxygen/Glucose Deprivation and Reperfusion Cause Modifications of Postsynaptic Morphology and Activity in the CA3 Area of Organotypic Hippocampal Slice Cultures
YeonJooJung, EunChengSuh, KyungEunLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.6 8 423-429 (7 pages)
synapsin I and phospho- synapsin. Brain Res. 2004;996:47-54. 32. S t o p p i n i L , B u c h s P A , M u l l e r D . A simple method for organotypic cultures of nervous tissue. J Neurosci Methods. 1991;37:173-182. 33. Ahlgren H, Henjum K, Ottersen OP, Runden-Pran E. Validation of organotypical hippocampal slice cultures as an ex vivo model of brain ischemia: different roles of NMDA receptors... Brain ischemia leads to overstimulation of N-methyl-D-aspartate (NMDA) receptors, referred as excitotoxicity, which mediates neuronal cell death. However, less attention has been paid to changes in synaptic activity and morphology that could have an important impact on cell function and survival following ischemic insult. In this study, we investigated the effects of reperfusion after oxygen/glucose deprivation (OGD) not only upon neuronal cell death, but also on ultrastructural and biochemical... -
Estrogen Pretreatment of Organotypic Hippocampal Slices Protects Neurons against Oxygen-Glucose Deprivation with Akt Activation
Eun-MiPark, SunghuiHong, Kyung-EunLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2006, Vol.10 No.3 2 123-130 (8 pages)
2001 Hurn PD, Macrae IM. Estrogen as a neuroprotectant in stroke. J Cereb Blood Flow Metab 20: 631-652, 2000 Jung YJ, Park SJ, Park JS, Lee KE. Glucose/oxygen deprivation induces the alteration of synapsin I and phosphosynapsin. Brain Res 996: 47-54, 2004 Kirino T, Sano K. Selective vulnerability in the gerbil hippocampus following transient ischemia. Acta Neuropathol (Berl) 62: 201- 208, 1984... In several experimental models, estrogens protect neurons against ischemic insults. However, the recent clinical studies of hormone replacement showed negative results to prevent stroke. Therefore, optimal models to study estrogen replacement for neuroprotection are needed before its clinical application. Organotypic hippocampal slice under oxygen-glucose deprivation (OGD) has been established as a model of cerebral ischemia and has advantages to study drug effects. We investigated whether... -
Kinesin Superfamily KIF5 Proteins Bind to βIII Spectrin
Jae-EunPaik, NariKim, SungSuYea, WonHeeJang, Joon-YoungChung, Sang-KyoungLee, Yeong-HongPark, JinHan, Dae-HyunSeog 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.3 8 167-172 (6 pages)
synapsin and GAP43, and dendrite cargo, such as messenger RNA and the AMPA receptor (Setou et al, 2002; Seog et al, 2004). In mice, the KIF5 subfamily was shown to include the distinctly brain-specific KIF5A (Aizawa et al, 1992) and KIF5C (Kanai et al, 2000), in addition to the ubiquitous KIF5B (Meng et al, 1997). Although both KIF5A and KIF5C are expressed in neurons, they ha ve different... The kinesin proteins (KIFs) make up a large superfamily of molecular motors that transport cargo such as vesicles, protein complexes, and organelles. KIF5 is a heterotetrameric motor that conveys vesicles and plays an important role in neuronal function. Here, we used the yeast two-hybrid system to identify the neuronal protein(s) that interacts with the tail region of KIF5 and found a specific interaction with βIII spectrin. The amino acid residues between 1394 and 1774 of βIII spectrin were... -
Presynaptic Mechanism Underlying Regulation of Transmitter Release by G Protein Coupled Receptors
TomoyukiTakahashi, YoshinaoKajikawa, MasahiroKimura, NaotoSaitoh, TetsuhiroTsujimoto 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.2 1 69-76 (8 pages)
with an overlap (right panel) with synapsin immuno- reactivity (middle panels, labeled red with Alexa fluor 568). Lowe r panels, absence in the immunoreactivity for Gi α1+2. Giα3 im- munoreactivity is also absent (see Kajikawa et al, 2001). Figure adopted from Kajikawa et al (2001) with permission from the National Academy of Sciences. ..PAGE:3 Potentiation of Inhibitory Action of Morphine 71... A variety of G protein coupled receptors (GPCRs) are expressed in the presynaptic terminals of central and peripheral synapses and play regulatory roles in transmitter release. The patch-clamp whole-cell recording technique, applied to the calyx of Held presynaptic terminal in brainstem slices of rodents, has made it possible to directly examine intracellular mechanisms underlying the GPCR-mediated presynaptic inhibition. At the calyx of Held, bath-application of agonists for GPCRs such as GABAB... -
신경전달물질 방출 저해제 FS11052가 신경세포와 PC12 세포의 돌기신장에 미치는 영향
이윤식, 김동섭, Lee. Yun-Sik, Kim. Dong-Seob 한국생명과학회 생명과학회지 8 Pages
한국생명과학회 생명과학회지 2006, Vol.16 No.2 315-322 (8 pages)
얇은 침상의 돌기가 세포체로부터 돌출되어 있었으며, growth cone 를 갖고 있지 않은 뉴우런이 많이 관찰되었다. FS11052 물질의 작용에 관해서는, 탈분극된 synaptic membrane이 $Ca^{2+}$ 이온을 유입 후 활성화되어 신경전달물질을 방출에 중요한 역할을 하고 있는 synaptotagmin, syntaxin, synapsin, SNAP25 등의 synaptosome을 구성하는 단백질에 직접 혹은 이와 밀접한 관련을 갖고 있는 인자와 간접적으로 작용하며, 신경전달물질의 방출을 억제하여 growth cone의 전향과 신경세포의 가소성을 조절하는 물질로 사료되어, 이...