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Effects of Electroacupuncture on Minimum Alveolar Concentration of Isoflurane and Cardiovascular System in Isoflurane Anesthetized Dogs
Seong-Mok Jeong 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 8 193-201 (9 pages)
(PVR) (Tables 3, 4, 6, 7, 8, 10 and 11). In the ST-36 group, no significant differences were observed for any cardiovascular parameter. Table 1. Analysis of variances of mean baseline minimum alveolar concentration of isoflurane among dogs with different washout period Sum of Squares df a Mean Square F b p Between groups Within groups Total 9.894E002 .160 .259 13 16 29 7.611E-03 1.000E-02 .761(NS... the minimum alveolar concentration (MAC) and on the cardiovascular system were evaluated with dogs under isoflurane anesthesia. Eight healthy male beagles were randomly assigned to six study groups (five heads/group) with washout intervals of 7 ~ 31 days between experiments for recovery and anesthetic clearance. MAC of isoflurane and cardiovascular parameters were determined after EA at nonacupoint and and at acupoints LI-4, SP-6, ST-36 and TH-8. Electroacupuncture for 30 minutes at LI-4, SP-6,... -
Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions
Ying-Xiao Fu, Jian-Hong Gu, Yi-Ran Zhang, Xi-Shuai Tong, Hong-Yan Zhao, Yan Yuan, Xue-Zhong Liu, Jian-Chun Bian, Zong-Ping Liu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 5 405-412 (8 pages)
inhibitory role. A previous report stated that OPG can regulate the expression of RANK mRNA in mouse osteoclast-like cells [6]. In the concentration and time gradient studies we conducted, OPG was found to attenuate RANK expression in RAW264.7 cells. This result was consistent with that of a study by Chen et al. [6]. However, the data in the present study showed that RANK mRNA expression... The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor κB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0, 10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast... -
Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Bock-Gie Jung, Sun-Ju Cho, Jae-Hyung Ko, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 6 213-220 (8 pages)
after two intradermal injections. ..PAGE:6 218 Bock-Gie Jung et al. Fig. 5. The inhibitory effects of interleukin-10 plasmid DNA. The concentration of serum IL-10 in mice injected with IL-10 plasmid DNA was significantly higher than in control mice. *p < 0.05 vs. control, **p < 0.01 vs. control. These results imply that the IL-10 plasmid DNA is functional in mammalian (mouse) cells and has a... eosinophil ratio and serum IgE concentration, and a reduction in the number of Staphylococcus aureus recovered from the ear. Moreover, relative mRNA expression levels of IL-4 and interferon-g in the skin lesions of mice injected with IL-10 plasmid DNA were also decreased compared with those of control mice. Of note, higher serum IL-10 levels in mice injected with IL-10 plasmid DNA were maintained compared with those in control mice. Taken together, the results indicate that IL-10 plasmid DNA can... -
Inhibitory effects of 2,6-di-tert-butyl-4-hydroxymethylphenol on asthmatic responses to ovalbumin challenge in conscious guinea pigs
Seul-YongJeong, Ji-YunLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.1 9 81-89 (9 pages)
in ethanol at 0.2 mM. Reaction so- lution was prepared by sequential addition of 1 ml buffer contain- ing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM EDTA; 10 μl of substrate (2 μM, final concentration); 10 μl of substrate (to 0.1% final concentration) and 6 μl of 1 M CaCl 2 (to 6 mM, final con- centration). The fluorescence of the reaction medium (black) was recorded and the reaction was... This study evaluated the anti-asthmatic activities of 2,6-di-tert-butyl-4-hydroxymethylphenol (DBHP) that is a potent phenolic antioxidant in edible vegetable oil. The effects of DBHP on bronchial asthma were evaluated by determining the specific airway resistance (sRaw) and tidal volume (TV) during the immediate asthmatic response (IAR) and the late-phase asthmatic response (LAR) in guinea pigs with aerosolized ovalbumin-induced asthma. Recruitment of leukocytes and the levels of biochemical... -
Cyanidin-3-glucoside Inhibits ATP-induced Intracellular Free Ca2+ Concentration, ROS Formation and Mitochondrial Depolarization in PC12 Cells
ShaziaPerveen, JiSeonYang, TaeJoungHa, ShinHeeYoon 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.4 3 297-305 (9 pages)
initial control (peak 2/peak 1) (n=28, 23, 25, 23, 26, 11 at 0 μμg/ml, 3 μg/ml, 10 μg/ml, 30 μg/ml, 100 μg/ml, respectively). A non-linear least- square fit of the Hill equation to the concentration-response data yielded an IC 50 of 15.3±0.1 μg/ml for cyani- din-3-glucoside. Data are means± SEM. duced [Ca 2 ]i increase in PC12 cells. Reproducible [Ca 2 ]i response could be elicite... have an ability to suppress various ion channels. We determined whether one of flavonoids, cyanidin-3-glucoside, affects adenosine 5'-triphosphate (ATP)-induced calcium signaling using digital imaging methods for intracellular free Ca2+ concentration ([Ca2+]i), reactive oxygen species (ROS) and mitochondrial membrane potential in PC12 cells. Treatment with ATP (100μM) for 90 sec induced [Ca2+]i increases in PC12 cells. Pretreatment with cyanidin-3-glucoside (1μg/ml to 100μg/ml) for 30 min... -
Inhibitory Actions of HERG Currents by the Immunosuppressant Drug Cyclosporin A
SeungHoLee, SangJuneHahn, GyesikMin, JimokKim, Su-HyunJo, HanChoe, BokHeeChoi 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.5 7 291-298 (8 pages)
syndrome; MEM, minimum essential medium. Received August 31, 2011, Revised October 17, 2011, Accepted October 19, 2011 Corresponding to: Bok Hee Choi, Department of Pharmacology, Institute for Medical Sciences, Chonbuk National University Medical School, San 2-20, Geumam-dong, Deokjin-gu, Jeonju 561-180, Korea. (Tel) 82-63-270-4250, (Fax) 82-63-275-2855, (E-mail) bhchoi@jbnu. ac.kr Inhibitory... concentration-dependent manner, with an IC50 value and a Hill coefficient of 3.17ՌM and 0.89, respectively. Pretreatment with cypermethrine, a calcineurin inhibitor, had no effect on the CsA-induced inhibition of the HERG channel. The CsA-induced inhibition of HERG channels was voltage-dependent, with a steep increase over the voltage range of the channel opening. However, the inhibition exhibited voltage independence over the voltage range of fully activated channels. CsA blocked the HERG... -
The Inhibitory Effects of Hydrogen Sulfide on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine
ShankarPrasadParajuli, SeokChoi, JunLee, YoungDaeKim, ChanGukPark, ManYooKim, HyunIlKim, CheolHoYeum, JaeYeoulJun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.2 4 83-89 (7 pages)
H 2S concentration of rat serum was <46 μM while the physiological concen- tration of H2S in rat brain tissue ha s been reported to be 50∼160 μM [5,8,9]. Actually, the maximum inhibitory ef- fects on pacemaker currents from ICC was gained using 1 mM NaHS, which is a relatively higher amount than that of endogenously expressed physiological concentration in mammalian tissues. But it was... in cultured mouse intestinal ICC. Exogenously applied sodium hydrogen sulfide (NaHS), a donor of hydrogen sulfide, caused a slight tonic inward current on pacemaker activity in ICC at low concentrations (50 and 100</SUP>ՌM), but at high concentration (500ՌM and 1 mM) it seemed to cause light tonic inward currents and then inhibited pacemaker amplitude and pacemaker frequency, and also an increase in the resting currents in the outward direction. Glibenclamide or other potassium... -
Effect of Extracts from Safflower Seeds on Osteoblastic Differentiation and Intracellular Free Calcium Concentration in MC3T3-E1 Cells
Hye-OckJang, Hyun-SupEom, Sung-BaeRoh, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.1 8 55-62 (8 pages)
action of safflower seed and (ii) to provide basic materials for designing new drug development which can prevent and cure bone deficiency diseases, including osteoporosis, with minimum side and toxic effects at a remarkably low cost with high efficacy. In the present study, we investigated the effects of the crude extract from Korean safflower seeds extracted by methyl alcoho l and an... Very little research has been carried out on safflower seed for the prevention and treatment of the bone deficiency diseases, including osteoporosis, which are supported by scientific evidences. In the present study, 3μl of 0.1% dried crude extract or 2μl of 0.1% dried aqueous fraction were shown to significantly accelerate the rate of differentiation of osteoblast. Also, the crude extract and aqueous fraction increased the [Ca2]i of the cultured osteoblast cells: 3μl of 0.1% dried... -
Increase of Intracellular Ca2+ Concentration Induced by Lysophosphatidylcholine in Murine Aortic Endothelial Cells
MeiHongZhu, SungJinPark, HyunJinKim, DongKiYang, SukHyoSuh, InsukSo, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.2 5 93-100 (8 pages)
Ca 2+ concentration were examined in mouse en dothelial cells by measuring intracellular Ca 2+ concentration ([Ca 2+]i) with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased [Ca 2+ ]i under the condition of 1.5 mM [Ca 2+ ]o but did not show any effect under the nominally Ca 2+ -free condition. Even after the store depletion with 30 μM 2,5-di-tert-... on intracellular Ca2 concentration were examined in mouse endothelial cells by measuring intracellular Ca2 concentration ([Ca2]i) with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased [Ca2]i under the condition of 1.5 mM [Ca2]o but did not show any effect under the nominally Ca2-free condition. Even after the store depletion with 30μM 2,5-di-tert- butylhydroquinone (BHQ) or 30μM ATP, LPC could still increase the... -
Inhibitory Activity of Sedum middendorffianum-Derived 4-Hydroxybenzoic Acid and Vanillic Acid on the Type III Secretion System of Pseudomonas syringae pv. tomato DC3000
Ji Eun Kang, Byeong Jun Jeon, Min Young Park, Beom Seok Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 8 608-617 (10 pages)
dimensional NMR such as 1H-NMR and 13C-NMR were obtained. Based on the data, structures of two compounds were determined. Effect of two phenolic compounds on growth of various plant pathogens. Minimum inhibitory concentration (MIC) of two phenolic acids was determined by serial dilution method in culture broth. Five serial two-fold dilutions of 4-hydroxybenzoic acid and vanillic acid were... to have inhibitory effect on promoter activity of hrpA gene encoding the structural protein of the T3SS apparatus. The phenolic acids at 2.5 mM significantly suppressed the expression of hopP1, hrpA, and hrpL in the hrp/hrc gene cluster without growth retardation of Pst DC3000. Auto-agglutination of Pst DC3000 cells, which is induced by T3SS, was impaired by the treatment of 4-hydroxybenzoic acid and vanillic acid. Additionally, 2.5 mM of each two phenolic acids attenuated disease symptoms...


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