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Potential erythropoiesis in the primo-vascular system in heart failure
Chae Jeong Lim, So Yeong Lee, Pan Dong Ryu 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 277 343-344 (2 pages)
in the primo-vascular system in heart failure Chae Jeong Lim 1, So Yeong Lee 1, Pan Dong Ryu *1 1Department of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea Introduction: The primo-vascular system (PVS), which consists of primo-vessels (PVs) and primo-nodes (PNs), is a novel... -
p53 signaling is involved in leptin-induced growth of hepatic and breast cancer cells
MohanShrestha, Pil-HoonPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.5 6 487-498 (12 pages)
www.kjpp.net 489 preparation of cDNA and real time-PCR amplification was then performed with a Roche Light Cycler 2.0 (Mannheim, Germany) using absolute qPCR SYBR green capillary mix AB gene system (Thermo Scientific, UK) at 95 oC for 15 min followed by 40 cycles at 95 oC for 15 s, 60 oC for 30 s and 72 oC for 30 s as described previously [20]. GAPDH was used as an internal control. The... Leptin, an adipokine predominantly produced from adipose tissue, is well known to induce tumor growth. However, underlying molecular mechanisms are not established yet. While p53 has long been well recognized as a potent tumor suppressor gene, accumulating evidence has also indicated its potential role in growth and survival of cancer cells depending on experimental environments. In the present study, we examined if p53 signaling is implicated in leptin-induced growth of cancer cells. Herein, we... -
Mutant p53-Notch1 Signaling Axis Is Involved in Curcumin-Induced Apoptosis of Breast Cancer Cells
Yun-HeeBae, JongHyoRyu, Hyun-JooPark, KwangRokKim, Hee-JunWee, Ok-HeeLee, Hye-OckJang, Moon-KyoungBae, Kyu-WonKim, Soo-KyungBae 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.4 6 291-297 (7 pages)
of Pharmacy, Seoul National University, Seoul 151-742, 5Severance Integrative Research Institute for Cerebral and Cardiovascular Diseases, Yonsei University Health System, Seoul 120-752, 6Medical Research Institute, Pusan National University, Busan 602-739, Korea Notch1 has been reported to be highly expressed in triple-negative and other subtypes of breast cancer. Mutant p53 (R280K) is... Notch1 has been reported to be highly expressed in triple-negative and other subtypes of breast cancer. Mutant p53 (R280K) is overexpressed in MDA-MB-231 triple-negative human breast cancer cells. The present study aimed to determine whether the mutant p53 can be a potent transcriptional activator of the Notch1 in MDA-MB-231 cells, and explore the role of this mutant p53-Notch1 axis in curcumin-induced apoptosis. We found that curcumin treatment resulted in an induction of apoptosis in... -
PR-domain containing protein 12 (Prdm12) is a downstream target of transcription factor Zic1 during brain cell differentiation
Byung Yong Park, Mahfujur Rahman, In shik Kim, Dong choon Ahn, Rashedunnabi Akanda 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 308 502-503 (2 pages)
formation of neural crest and cerebellar development where its main function is neuronal cell differentiation. Studies reported that prdm family is expressed in the nervous system of developing mice and zebrafish. However, the function of prdm in neurogenesis is still unclear. Here, we identified prdm12 in Xenopus as a downstream of zic1 transcription factor. We propose that prdm12 is a... -
STB-HO, a novel mica fine particle, inhibits the teratoma-forming ability of human embryonic stem cells after in vivo transplantation
Soon Won Choi, Tae Hoon Shin, Ji Hee Shin, Byung Chul Lee, In sung Kang, Hafiz Uddin, Yoo jin Seo, Hyung Sik Kim, Kyung Sun Kang 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 306 501-502 (2 pages)
formation of neural crest and cerebellar development where its main function is neuronal cell differentiation. Studies reported that prdm family is expressed in the nervous system of developing mice and zebrafish. However, the function of prdm in neurogenesis is still unclear. Here, we identified prdm12 in Xenopus as a downstream of zic1 transcription factor. We propose that prdm12 is a... -
Estrogen receptor-α, progesterone receptor, and c-erbB/HER-family receptor mRNA detection and phenotype analysis in spontaneous canine models of breast cancer
Farruk M. Lutful Kabir, Patricia DeInnocentes, Payal Agarwal, Christopher P. Mill, David J. Riese 2nd, R. Curtis Bird 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 4 149-158 (10 pages)
Bio-Rad iCycler iQ Multicolor PCR Detection System using an ABsoluteTM QPCR SYBR Green Fluorescein Mix (Thermo Scientific, USA) [12]. The first QrtPCR step began with reverse transcription of total RNA (0.5g) from each cell line being added to a reaction mixture containing a reverse transcription supermix (Bio-Rad Laboratories, USA). Final 20 L reactions were incubated for 5 min at 25 oC, 30... Well characterized, stable, p16-defective canine mammary cancer (CMT) cell lines and normal canine mammary epithelial cells were used to investigate expression of the major breast cancer-specific hormone receptors estrogen receptor alpha (ER1) and progesterone receptor (PR) as well as luminal epithelial-specific proto-oncogenes encoding c-erbB-1 (epidermal growth factor receptor/EGFr), c-erbB-2/HER2, c-erbB-3, and c-erbB-4 receptors. The investigation developed and validated quantitative reverse... -
Vitamin D3 up-regulated protein 1 controls the priming phase of liver regeneration
Hyo-Jung Kwon, Sung-Kuk Hong, Won-Kee Yoon, Ki-Hoan Nam, In-Pyo Choi, Dae-Yong Kim, Hyoung-Chin Kim, Young-Suk Won 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 4 257-262 (6 pages)
(PCR) using SYBR Green PCR master mix (Applied Biosystems, USA) and target- specific primers (Table 1). Reactions were amplified and quantified using the ABI 7700 sequence detection system and the manufacturer’s software (Applied Biosystems). Target mRNA expression levels were normalized to those of β-actin, which served as an internal control. Western blot analysis Equal amounts of... Vitamin D3 up-regulated protein 1 (VDUP1) is a potent growth suppressor that inhibits tumor cell proliferation and cell cycle progression when overexpressed. In a previous study, we showed that VDUP1 knockout (KO) mice exhibited accelerated liver regeneration because such animals could effectively control the expression of cell cycle regulators that drive the G1-to-S phase progression. In the present study, we further investigated the role played by VDUP1 in initial priming of liver... -
Prolonged expression of senescence markers in mice exposed to gamma-irradiation
Min-A Seol, Uhee Jung*, Hyeon Soo Eom, Seol-Hwa Kim, Hae-Ran Park, Sung-Kee Jo 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 1 331-338 (8 pages)
in a 1.2% agrarose gel and stained with ethidium bromide. The DNA bands were visualized under UV light using Kodak EDAS 290 digital imaging system (Eastman Kodak, USA). Intensity of the amplified DNA bands was quantified using Kodak ID 3.6 software (Eastman Kodak, USA). Western blotting The tissues were homogenized with Omni TH tissue homogenizer (Omni International, USA) in a 1 mL of... Although ionizing radiation is known to induce cellular senescence in vitro and in vivo, its long-term in vivo effects are not well defined. In this study, we examined the prolonged expression of senescence markers in mice irradiated with single or fractionated doses. C57BL/6 female mice were exposed to 5 Gy of γ-rays in single or 5, 10, 25 fractions. At 2, 4, and 6 months after irradiation, senescence markers including mitochondrial DNA (mtDNA) common deletion, p21, and senescence-associated... -
Mutation and overexpression of p53 as a prognostic factor in canine mammary tumors
Chung-Ho Lee, Wan-Hee Kim, Ji-Hey Lim, Min-Soo Kang, Dae-Yong Kim and Oh-Kyeong Kweon 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 9 63-69 (7 pages)
p53 positive if nuclear staining was clearly detected, but cytoplasmic staining alone was not recorded as positive. Positively staining was evaluated semi-quantitatively using a previously described system where 0 = no staining; 1 = <10%; 2 = 10-50%; and 3 = >50% of cells. Based upon previous reports [25,29], we considered tumors to be p53 positive by receiving 2 or 3 score. Proliferative activity... We concentrated ourselves to evaluate the prognostic significance of the p53 gene mutations, its protein expression and MIB-1 index as a proliferative marker in canine mammary tumors. In the present study, a total of 20 cases were examined, among which there were 5 malignant mixed tumors, 4 mammary gland adenocarcinomas, 1 papillary adenocarcinoma, 8 benign mixed tumors and 2 mammary gland adenomas. Positive immunostaining for p53 with PAb240 antibody was found in 2 benign (20%) and 3 malignant... -
Proteasome 억제에 의한 P53의 발현과 미토콘드리아 막 전압의 소실로 TRAIL에 저항하는 폐암세포의 사멸 강화
설재원, 박상열 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2009, 제 49권 제 1호 1 1-8 (8 pages)
이 과정을 ubiquitin- proteasome system(UPS)라 한다. 이러한 UPS를 통한 세 포내 단백질의 분해는 세포의 증식과 사멸을 조절하는 데 중요한 역할을 하는 것으로 알려져 있지만, UPS에 의한 세포의 증식과 사멸 등의 상반되는 영향에 대해서 는 아직까지 많은 논란이 되고 있다 [17, 31 ]. Proteasome은 multicatalytic protease complex로서 lysosome에 의존하지 않고 ubiqutin과 ATP에 의존하여 *Corresponding author:...