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전체 선택해제
총 696건 검색
본문검색 결과만 보기
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16S-23S rRNA Intergenic Spacer Region을 이용한 Vibrio ichthyoenteri Species-specific Primer 개발
문영건, 허문수, Moon. Young-Gun, Heo. Moon-Soo 한국미생물학회 미생물학회지 8 Pages
한국미생물학회 미생물학회지 2005, Vol.41 No.2 117-124 (8 pages)
348bp이며 tRNA gene을 가지고 있지 않았다. 이 서열을 가지고 이미 알려진 다른 Vibrio 종의 ISR 서열과 mutiple alignment를 수행한 결과 여러 영역에서 높은 가변성을 나타내어 가변 부위를 표적으로 하여 V. ichthyoenteri를 검출하기 위한 종 특이적 primer를 제작하였다. 제작된 primer의 특이성을 확인하기 위해 Vibrio 표준균주 19종의 genomic DNA와 분리균주 18 group에 genomic DNA 그리고 V. ichthyoenteri와 가장 유사한 서열을 가지고 있다고 알려진 Vibrio 종의 genomic DNA를 가지고 시험하였다. 그 결과 본 연구에서... -
Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 2 212-218 (7 pages)
of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the... -
Species-specific Detection of Erwinia pyrifoliae by PCR Assay Using Enterobacterial Repetivive Intergenic Consensus (ERIC) Primers
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 10 267-270 (4 pages)
We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens. -
Development of Streptococcus sanguinis-, Streptococcus parasanguinis-, and Streptococcus gordonii-PCR Primers Based on the Nucleotide Sequences of Species-specific DNA Probes Screened by Inverted Dot Blot Hybridization
Park. Soon-Nang, Kook. Joong-Ki 대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 7 Pages
대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 2013, Vol.38 No.2 43-49 (7 pages)
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Identification and Detection of Streptococcus anginosus Using Species-Specific 16S rDNA Primers
Cho. Ji-Sun, Yoo. So-Young, Kim. Hwa-Sook, Hwang. Ho-Keel, Min. Jeong-Beom, Kim. Byung-Hoon, Baek. Dong-Heon, Shin. Hwan-Seon, Kook. Joo 대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 4 Pages
대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 2006, Vol.31 No.1 11-14 (4 pages)
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Comparison of allele-specific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle
Kozet Avanus, Ahmet Altınel 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 9 465-470 (6 pages)
cattle. The aim of this study was to compare sensitivity, specificity, positive and negative predictive values, accuracy, and rapidity of allele-specific polymerase chain reaction (AS-PCR), created restriction-site PCR (CRS-PCR), and PCR with primer-introduced restriction analysis (PCR-PIRA), three methods used in identification of CVM carriers in a Holstein cattle population. In order to screen for the G＞T mutation in the solute carrier family 35 member A3 (SLC35A3) gene, DNA sequencing as the... -
Sensitive, Accurate PCR Assays for Detecting Harmful Dinoflagellate Cochlodinium polykrikoides Using a Specific Oligonucleotide Primer Set
Kim. Chang-Hoon, Park. Gi-Hong, Kim. Keun-Yong 한국수산과학회 Journal of fisheries science and technology 8 Pages
한국수산과학회 Journal of fisheries science and technology 2004, Vol.7 No.3 122-129 (8 pages)
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Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds
Chae-yeong Lee, Hyun-Hee Lee, Mohamed Mannaa, Nam-gyu Kim, Jung-wook Park, Ju-yun Kim, Young-Su Seo 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 4 490-498 (9 pages)
The bacteria have been reported as seed-borne pathogens from rice. Accurate detection of both pathogens on the seeds is very important for limiting the disease dissemination. Novel primer pairs targeting specific molecular markers were developed for the robust detection of B. glumae and B. gladioli. The designed primers were specific in detecting the target species with no apparent crossreactions with other related Burkholderia species at the expected product size. Both primer pairs displayed a... -
Occurrence of the Root-Knot Nematode species in Vegetable Crops in Souss Region of Morocco
Soukaina Janati, Abdellah Houari, Ahmed Wifaya, Adil Essarioui, Abdelaziz Mimouni, Abderrahim Hormatallah, Mohamed Sbaghi, Abdelfattah 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 4호 5 308-315 (8 pages)
as molecularlly using species-specific primers. The obtained results indicated that Meloidogyne javanica and M. incognita were identified in 86.4% and 13.5% of the total populations, respectively. The lowest incidence of root-knot nematodes (64%) was found in Toussous, whereas the highest frequencies of 100% and 90% were detected in Taddart and Biogra, respectively. As the majority of the samples have been infested with Meloidogyne species; this indicates that there is an urgent need to provide... -
Specific PCR Detection of Four Quarantine Fusarium Species in Korea
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 16 409-416 (8 pages)
method for detecting four Fusarium species in quarantined plants in Korea: F. solani f. sp. cucurbitae, F. stilboides, F. redolens, and F. semitectum var. majus. Species-specific primers were designed from the nucleotide sequences of either the translation elongation factor-1 alpha (TEF1) gene or RNA polymerase II subunit (RPB2) gene. Two different primer sets derived from TEF1, all specific to F. solani f. sp. cucurbitae, were able to differentiate the two races (1 and 2) of this species. A set... -
ISMAP02-like sequence in Mycobacterium terrae complex interferes specific detection of Mycobacterium avium subsp. Paratuberculosis
Hong Tae Park, Hyun Eui Park, Myung hwan Jung, Soo Jin Shim, Young Hoon Jung, Han Sang Yoo 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 192 296-296 (1 pages)
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The current epidemiological status of infectious coryza and efficacy of PoulShot Coryza in specific pathogen-free chickens
Moo-Sung Han, Jong-Nyeo Kim, Eun-Ok Jeon, Hae-Rim Lee, Bon-Sang Koo, Kyeong-Cheol Min, Seung-Baek Lee, Yeon-Ji Bae, Jong-Suk Mo, 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 8 323-330 (8 pages)
Infectious coryza (IC) is an infectious disease caused by Avibacterium (Av.) paragallinarum. IC is known to cause economic losses in the poultry industry via decreased egg production in layers. Between 2012 and 2013, Av. paragallinarum was isolated from seven chicken farms by Chungbuk National University. We identified Av. paragallinarum, the causative pathogen of IC by polymerase chain reaction (PCR) and serovar serotype A, by multiplex PCR. Antibiotic sensitivity tests indicated that a few... -
A core set of microsatellite markers for conservation genetics studies of Korean goral (Naemorhedus caudatus) and its cross-species amplification in Caprinae species
Junghwa An, Sung-Kyoung Choi, Julie Sommer, Edward Louis Jr, Rick Brenneman, Barbora Zemanov?, Petra H?jkov?, Grimm Park, Mi-Soo 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 12 351-353 (3 pages)
In order to screen microsatellites for conservation genetics studies of the species, a total of 23 microsatellite loci from Korean goral (Naemorhedus caudatus), including 15 previously developed loci and 8 new loci in this study, were tested. Eleven microsatellites were screened and subjected to cross-species amplification using a test panel of four Caprinae species, Japanese serows (Capricornis crispus), Chinese gorals (Naemorhedus goral), Northern chamois (Rupicapra rupicapra) and domestic... -
Current Classification of the Bacillus pumilus Group Species, the Rubber- Pathogenic Bacteria Causing Trunk Bulges Disease in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches
Ainur Ainiah Azman Husni, Siti Izera Ismail, Noraini Md. Jaafar, Dzarifah Zulperi 한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 4 243-257 (15 pages)
Bacillus pumilus is the causal agent of trunk bulges dis- ease affecting rubber and rubberwood quality and yield production. In this study, B. pumilus and other closely related species were included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA analysis. Multilocus sequence analysis (MLSA) of five housekeeping genes and repetitive elements-based poly- merase chain reaction (rep-PCR) using REP, ERIC, and BOX primers conducted to analyze the diversity and systematic... -
Detection of Blackleg Resistance Gene Rlm1 in Double-Low Rapeseed Accessions from Sichuan Province, by Kompetitive Allele-Specific PCR
Liang Chai, Jinfang Zhang, Wannakuwattewaduge Gerard Dilantha Fernando, Haojie Li, Xiaoqin Huang, Cheng Cui, Jun Jiang, Benchuan Zheng, Yong Liu, Liangcai Jiang 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 10 194-199 (6 pages)
Blackleg is a serious disease in Brassica plants, causing moderate to severe yield losses in rapeseed worldwide. Although China has not suffered from this disease yet (more aggressive Leptosphaeria maculans is not present yet), it is crucial to take provisions in breeding for dis- ease resistance to have excellent blackleg-resistant cul- tivars already in the fields or in the breeding pipeline. The most efficient strategy for controlling this disease is breeding plants with identified resistance... -
HR-Mediated Defense Response is Overcome at High Temperatures in Capsicum Species
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 1호 8 71-77 (7 pages)
Resistance to Tomato spotted wilt virus isolated from paprika (TSWV-Pap) was overcome at high temperatures (30 ± 2°C) in both accessions of Capsicum annuum S3669 (Hana Seed Company) and C. chinense PI15225 (AVRDC Vegetable Genetic Resources). S3669 and PI15225, which carrying the Tsw gene, were mechanically inoculated with TSWV-Pap, and then maintained in growth chambers at temperatures ranging from 15 ± 2°C to 30 ± 2°C (in 5°C increments).... -
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 4 123-131 (9 pages)
of a subspecies-specific PCR primer set (Cms89F/R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least 1.47×10 copies/μl of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or 10...
