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간행물
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Characterization of a Chitinase Gene Exhibiting Antifungal Activity from a Biocontrol Bacterium Bacillus licheniformis N1
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 4호 6 344-351 (8 pages)
These results indicated that even though the chitinase gene is not expressed in the N1 strain, the coding region is functional and encodes an active chitinase enzyme. Furthermore, B. subtilis 168 transformants expressing the chitinase gene exhibited antifungal activity against Fulvia fulva by suppressing spore germination. Our results suggest that the proper engineering of the expression of the indigenous chitinase gene, which will lead to its expression in the biocontrol strain B. licheniformis... -
The Biocontrol Activity of Chromobacterium sp. Strain C-61 against Rhizoctonia solani Depends on the Productive Ability of Chitinase
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2005, 21권 3호 12 275-282 (8 pages)
causal agent of damping-off of eggplant. In this study, the biocontrol activity and enzymatic characteristics of strain C-61 were compared with its four Tn5 insertion mutants (C61-A, -B, -C, and -D) that had lower chitinolytic ability. The chitinase activity of a 2-day old culture was about 76%, 49% and 6% level in C61-A, C61-B and in C61-C, respectively, compared with that of strain C-61. The β-N-acetylhexosaminidase (Nahase) activity was little detected in strain C-61 but increased largely in... -
Molecular Screening of Streptomyces Isolates for Antifungal Activity and Family 19 Chitinase Enzymes
Gherbawy. Youssuf, Elhariry. Hesham, Altalhi. Abdulla, El-Deeb. Bahig, Khiralla. Ghada 한국미생물학회 The journal of microbiology 10 Pages
한국미생물학회 The journal of microbiology 2012, Vol.50 No.3 459-468 (10 pages)
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Analysis of the Involvement of Chitin-Binding Domain of ChiCW in Antifungal Activity, and Engineering a Novel Chimeric Chitinase with High Enzyme and Antifungal Activities
Huang. Chien-Jui, Guo. Shu-Huei, Chung. Shu-Chun, Lin. Yu-Ju, Chen. Chao-Ying 한국미생물생명공학회 Journal of microbiology and biotechnology 7 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2009, Vol.19 No.10 1169-1175 (7 pages)
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Effect of Introducing Chitinase Gene on the Resistance of Tuber Mustard against White Mold
Seyedmohammadreza Ojaghian, Ling Wang, Guan-Lin Xie 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 8 378-383 (6 pages)
lines TMB4, TMB7, TMB12, and TMB18, respectively. Enzymatic tests showed a significant increase in the activity of endochitinase in protein isolated from leaf tissues of chit42 transgenic 75-day tuber mustard lines. The pathogenicity of three pathogen isolates was tested on the leaves of transformed plans. The results of current study showed that expression of the gene chit42 in tuber mustard plants markedly reduced infection radius on the leaves 7 days after inoculation with the fungus. -
Genomic Insights into Nematicidal Activity of a Bacterial Endophyte, Raoultella ornithinolytica MG against Pine Wilt Nematode
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 3호 11 250-255 (6 pages)
xylophilus. Our analysis of R. ornithinolytica MG genome showed that it possesses many genes encoding potential nematicidal factors in addition to some secondary metabolite biosynthetic gene clusters that may contribute to the observed nematicidal activity of the strain. Furthermore, the genome was lacking key components of avermectin gene cluster, suggesting that nematicidal activity of the bacterium is not likely due to the famous anthelmintic agent of wide-spread use, avermectin. This genomic... -
Purification and Characterization of a Major Extracellular Chitinase from a Biocontrol Bacterium, Paenibacillus elgii HOA73
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 10 318-328 (11 pages)
Chitinase-producing Paenibacillus elgii strain HOA73 has been used to control plant diseases. However, the antimicrobial activity of its extracellular chitinase has not been fully elucidated. The major extracellular chitinase gene (PeChi68) from strain HOA73 was cloned and expressed in Escherichia coli in this study. This gene had an open reading frame of 2,028 bp, encoding a protein of 675 amino acid residues containing a secretion signal peptide, a chitin-binding domain, two fibronectin type... -
Expression of Rice Chitinase Gene in Genetically Engineered Tomato Confers Enhanced Resistance to Fusarium Wilt and Early Blight
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 6 252-258 (7 pages)
lines of tomato harboring rice chitinase (RCG3) gene for resistance to two important fungal pathogens Fusarium oxysporum f. sp. lycopersici (Fol) causing fusarium wilt and Alternaria solani causing early blight (EB). In this study, three transgenic lines TL1, TL2 and TL3 of tomato Solanum lycopersicum Mill. cv. Riogrande genetically engineered with rice chitinase (RCG 3) gene and their R1 progeny was tested for resistance to Fol by root dip method and A. solani by detached leaf assay. All the R0... -
Insight Into Genes Involved in the Production of Extracellular Chitinase in a Biocontrol Bacterium Lysobacter enzymogenes C-3
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 4호 13 439-445 (7 pages)
The chitinase producing Lysobacter enzymogenes C-3 has previously been shown to suppress plant pathogens in vitro and in the field, but little is known of the regulation of chitinase production, or its role in antimicrobial activity and biocontrol. In this study, we isolated and characterized chitinase-defective mutants by screening the transposon mutants of L. enzymogenes C-3. These mutations disrupted genes involved in diverse functions: glucose-galactose transpoter (gluP), disulfide bond... -
Research Articles : Purification and Structural Analysis of Surfactin Produced by Endophytic Bacillus subtilis EBS05 and its Antagonistic Activity Against Rhizoctonia cerealis
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 6 342-348 (7 pages)
th of strain EBS05 can more effectively control WSED than can fludioxonil. The fermentation broth and bacteria-free filter ability to suppress WSED was not significantly different, suggesting that an active secreted substance played a major role in controlling WSED. Separation and purification of the active compounds was carried out by serial processes, including hydrochloric acid (pH 2.0) treatment, methanol extraction and Sephadex LH-20 column chromatography, silica gel column chromatography... -
Production of Surfactin and Iturin by Bacillus licheniformis N1 Responsible for Plant Disease Control Activity
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 2호 10 170-177 (8 pages)
activity. Two N1E based formulations, bacterial cell based formulation PN1E and culture supernatant based formulation SN1E, were evaluated for disease control activity against gray mold disease of tomato and strawberry plants. Neither PN1E nor SN1E was as effective as the original formulation N1E. Fractionation of antifungal compounds from the bacterial culture supernatant of B. licheniformis N1 indicated that two different cyclic lipopeptides were responsible for the antimicrobial activity of... -
Effect of Acibenzolar-S-methyl and Rahnella aquatilis (Ra39) on Chitinase and β-1, 3-glucanase Activities and Disease Resistance of Apple Plants
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 9 63-69 (7 pages)
In physiological studies, enhanced activities of PR-proteins (chitinase and β-1, 3-glucanase) were detected, which are well known as biochemical markers for systemic acquired resistance. Application of ASM to apple shoots caused the highest chitinase activity followed by strain Ra39. The enzyme activity was increased after 2, 4 and 6 days from application. In addition, ASM-treatment caused the higher β-1, 3-glucanase activity than strain Ra39. Maximum enzyme activity was recorded after 6 days... -
Trichoderma harzianum SJG-99721의 체외 분비 chitinase 생산에 미치는 생물 반응기에서의 반응 최적화 연구
한국환경생물학회 환경생물 2004, Vol.22 No.1 167-170 (4 pages)
실험만으로 최적의 온도 혹은 pH 및 폭기율과 교반양을 구할 수 있었다. 그 결과 온도 $32^{circ}C$, pH4.9, 폭기율 3.22 ι, 교반율 225 rpm에서 chitinase의 최대 생산량 16.825 U를 나타내었다. 이는 온도 $25^{circ}C$, pH 5, 폭기율 1l, 교반율 150 rpm에서 나타난 chitinase 생산량 4.221 U에 비교하면 3.99배 증가한 효과를 나타내고 있다. 이로서 self-directing optimization방법을 통한 최적화 기술이 효소 생산 기술에 있어 그 최적화 과정을 단축시키고 최종 과정에 도달하는 새로운 기술로 적용할 수 있음을 알 수 있었다. -
부추첨가 김치의 발효과정 중 chitinase 활성과 조직감
김유경, 이귀주 한국식품조리과학회 한국조리과학회지 6 Pages
한국식품조리과학회 한국조리과학회지 1999, Vol.15 No.2 102-107 (6 pages)
숙성이 진행됨에 따라 점차적으로 감소한 반면, 김치조직에서의 chitinase 활성은 숙성 3일 또는 5일까지 증가한 후 감소하였다. 그러나 김치조직과 김치액 모두에서 대조구보다 부추첨가구가 더 높은 chitinase 활성을 나타내었으며 부추첨가구에서는 부추첨가량이 증가할수록 높은 chitinase 활성을 보였다. 숙성이 진행됨에 따라 모든 시료구에서는 김치조직의 침투관통력은 감소하였으며 부추첨가량에 따른 차이는 미미하였으나 대조구가 숙성 전 기간동안 가장 낮은 침투관통력을 보였다. 이상의 결과를 종합해보면 부추첨가는... -
황토로부터 분리한 Bacillus licheniformis의 항진균 chitinase 생산과 효소 특성
한귀환, 봉기문, 김종민, 김평일, 김시욱, Han. Gui Hwan, Bong. Ki Moon, Kim. Jong Min, Kim. Pyoung Il, Kim. Si Wouk 한국생물공학회 KSBB Journal 8 Pages
한국생물공학회 KSBB Journal 2014, Vol.29 No.3 131-138 (8 pages)
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Antifungal Activity of Extracellular Hydrolases Produced by Autolysing Aspergillus nidulans Cultures
Szilagyi. Melinda, Anton. Fruzsina, Forgacs. Katalin, Yu. Jae-Hyuk, Pocsi. Istvan, Emri. Tamas 한국미생물학회 The journal of microbiology 6 Pages
한국미생물학회 The journal of microbiology 2012, Vol.50 No.5 849-854 (6 pages)


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