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Cloning and Characterization of Cellulase Gene (cel5C) from Cow Rumen Metagenomic Library
김민근, 디렌 바르만, 강태호, 김정호, 김훈, 윤한대, Kim. Min-Keun, Barman. Dhirendra Nath, Kang. Tae-Ho, Kim. Jung-Ho, Kim. Hoon, Yun. Han-Dae 한국생명과학회 생명과학회지 10 Pages
한국생명과학회 생명과학회지 2012, Vol.22 No.4 437-446 (10 pages)
한우의 반추위에서 게놈 DNA를 분리하여 메타게놈 은행을 구축한 다음 섬유소분해효소를 암호화하는 유전자를 클로닝 및 유전자를 선별하였다. 선별된 유전자의 DNA 염기서열 및 아미노산 서열을 분석하고 유전산물의 생화학적인 특성을 조사하였다. $cel$5C 유전자는 1,125 bp로 374개의 아미노산 잔기를 가진 단백질을 암호화하였으며 이 단백질 분자량은 42 kDa이었다. 이 효소의 최적 pH는 4 근방이었으며 최적 온도는 $50^{circ}C$ 부근이었다. $cel$5C 유전자의 internal primer를 사용하여 인공적으로 배양할 수 있는 49종의... -
Cloning and Characterization of a Novel Carboxylesterase Gene from Cow Rumen Metagenomic Library
아스라풀 이스람, 김민근, 아라디아, 스리니 래디, 김은진, 김정호, 김훈, 윤한대, Asraful Islam. Shah Md., Kim. Min-Keun, Renukaradhya. K. Math, Srinivasa. Reddy R.N., Kim. E 한국생명과학회 생명과학회지 8 Pages
한국생명과학회 생명과학회지 2010, Vol.20 No.9 1306-1313 (8 pages)
한우의 반추위에서 게놈 DNA를 분리하여 메타게놈 은행을 구축한 다음 carboxylesterase를 암호화하는 유전자를 클로닝 및 유전자를 선별하였다. 선별된 유전자의 DNA 염기서열 및 아미노산 서열을 분석하고 유전산물의 생화학적인 특성을 조사하였다. est1R 유전자는 2,465 bp로 366개의 아미노산 잔기를 가진 단백질을 암호화하였으며 이 단백질의 이론적인 분자량은 61,166 Da이었다. Est1R단백질은 PNB carboxylesterase (P37967), acetylcholinesterase (1EEAA) 및 chain A (1H23A)와 각각 5.9%, 6.1%, 6.1% 상동성을 가지고 있었다.... -
Cloning of α-Amylase Gene from Unculturable Bacterium Using Cow Rumen Metagenome
조수정, 윤한대, Cho. Soo-Jeong, Yun. Han-Dae 한국생명과학회 생명과학회지 9 Pages
한국생명과학회 생명과학회지 2005, Vol.15 No.6 1013-1021 (9 pages)
미생물 메타게놈은 특이한 생체촉매의 다양한 원료로 제공된다. 한우의 반추위에서 게놈 DNA를 분리한 후 메타게놈 은행을 구축하고 $alpha$-amlylase를 암호화하는 유전자를 클로닝하여 DNA 및 아미노산 서열을 밝히고 생화적 특징을 조사하였다. amyA유전자는 1,893 bp로 631개의 아미노산 잔기를 가진 단백질을 암호화였으며 효소의 분자량은 단백질 전기영동 결과 약 71,000 Da으로 확인되었다. 이 효소를 다른 아밀라제와 비교한 결과 $21-59\%$의 상동성을 보였다. AnyA는 pH $6.40\%$에서 최적 활성을 나타내었고, pl값은... -
16S rDNA Analysis 9f Bacterial Diversity in Three Fractions of Cow Rumen
CHO. Soo-Jeong, CHO. Kye-Man, SHIN. Eun-Chule, LIM. Woo-Jin, HONG. Su-Young, CHOI. Byoung-Rock, Kang. Jung-Mi, LEE. Sun-Mi, KIM. Yong-He 한국미생물생명공학회 Journal of microbiology and biotechnology 10 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2006, Vol.16 No.1 92-101 (10 pages)
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Left abomasal displacement between the uterus and rumen during bovine twin pregnancy
Bart Pardon*, Geert Vertenten, Pieter Cornillie, Stijn Schauvliege, Frank Gasthuys, Gunther van Loon, Piet Deprez 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 16 437-440 (4 pages)
distension, but no ping sounds. An unusually high position of the gravid uterine horn on the left side was observed with ultrasound. Left exploratory laparotomy confirmed that the abomasum was displaced to the left and entrapped between the rumen and twin gravid uterine horn. A left surgical approach was necessary to correct the condition. Both animals recovered and gave birth to healthy twins. The present cases indicate that the subomental position of a heavy twin gravid uterine horn is a... -
Cloning and Identification of a New Group Esterase (Est5S) from Noncultured Rumen Bacterium
Kim. Min Keun, Kang. Tae Ho, Kim. Jungho, Kim. Hoon, Yun. Han Dae 한국미생물생명공학회 Journal of microbiology and biotechnology 10 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2012, Vol.22 No.8 1044-1053 (10 pages)
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Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats
Santi Devi Upadhaya, Ha Guyn Sung, Chan Hee Lee, Se Young Lee, Sun Woo Kim, Kyung Jin Cho, Jong K. Ha* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 5 29-34 (6 pages)
by collecting rumen fluid at different times (0, 3, 6, 9 and 12 h) after feeding. Cannulated Holstein steers (740 ± 10 kg bw) and Korean native goats (26 ± 3 kg bw) were fed a 60% timothy and 40% commercial diet with free access to water. Rumen fluid from Korean native goats demonstrated higher (p ＜ 0.01) aflatoxin B1 degradability than Holstein steers. However, filtration method had no significant influence on degradability. In addition, aflatoxin degradation did not depend upon rume... -
Ultrasonographic ovarian dynamic, plasma progesterone, and non-esterified fatty acids in lame postpartum dairy cows
Pedro Melendez, Veronica Gomez, Hans Bothe, Francisco Rodriguez, Juan Velez, Hernando Lopez, Julian Bartolome, Louis Archbald 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 16 462-467 (6 pages)
The objective of this study was to compare ovulation rate, number of large ovarian follicles, and concentrations of plasma progesterone (P4) and non-esterified fatty acids (NEFA) between lame (n = 10) and non-lame (n = 10) lactating Holstein cows. The study was conducted in an organic dairy farm, and cows were evaluated by undertaking ultrasonography and blood sampling every 3 days from 30 days postpartum for a period of 34 days. Cows which became lame during the first 30 days postpartum... -
Retrospective surveillance of metabolic parameters affecting reproductive performance of Japanese Black breeding cows
Urara Watanabe, Mitsuhiro Takagi*, Osamu Yamato, Takeshige Otoi, Koji Okamoto 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 15 283-288 (6 pages)
This retrospective study was conducted to confirm the relationship between pre- and postpartum metabolic parameters and postpartum reproductive performance and to clarify seasonal characteristics of the metabolic parameters by using our metabolic profile test (MPT) database of Japanese Black breeding herds. In evaluation 1, MPT databases of blood samples from multiparous cows collected prepartum and postpartum were divided into two groups according to calving interval, and each MPT parameter was... -
Oxidative response of neutrophils to platelet-activating factor is altered during acute ruminal acidosis induced by oligofructose in heifers
Claudia Concha, María Daniella Carretta, Pablo Alarcón, Ivan Conejeros, Diego Gallardo, Alejandra Isabel H 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 7 217-224 (8 pages)
Reactive oxygen species (ROS) production is one of the main mechanisms used to kill microbes during innate immune response. D-lactic acid, which is augmented during acute ruminal acidosis, reduces platelet activating factor (PAF)-induced ROS production and L-selectin shedding in bovine neutrophils in vitro. This study was conducted to investigate whether acute ruminal acidosis induced by acute oligofructose overload in heifers interferes with ROS production and L-selectin shedding in blood... -
Analgesic effect of caudal epidural ketamine in cattle
Inhyung Lee, Tomo Yoshiuchi, Norio Yamagishi, Kenji Oboshi, Yu Ayukawa, Naoki Sasaki, Haruo Yamada 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 10 261-264 (4 pages)
1 week interval: 5, 10 and 20 mL of 5% ketamine. Sedation, analgesia, ataxia and other effects on cardiopulmonary and rumen functions were assessed before ketamine administration and until 120 min. The analgesia without sedation was shown at tail and perineum about 5 min after all three treatments. The duration of analgesia was significantly increased according to the volume of ketamine (p < 0.01). There was a similar tendency of ataxia with individual variation. There were minimal effects on... -
Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods
I. U. Khan, A. A. Hassan, A. Abdulmawjood, C. Lammler, W. Wolter, M. Zschock 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 3 213-223 (11 pages)
by macrorestriction analysis of their chromosomal DNA by pulsed-field gel electrophoresis, revealed that most of the isolates were not related to each other. However, identical DNA patterns were noted for some of the isolates within different quarters of an individual cow and also for different cows within the same farm. The generally unrelated DNA patterns indicated that S. uberis is a pathogen with multiple environmental habitats and that infections are caused by a great variety of strains. -
Biological Rhythm in Livestock
Piccione Giuseppe and Caola Giovanni 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 1 145-157 (13 pages)
The animal time structure is a basic fact of life, no matter if one wants to study it or not. The time-dependent, mostly rhythmic, and thus to a certain degree predictable, variations of biochemical and physiological functions and of sensitivity and resistance to many environmental agents are often quite large and offer not only new insight into animal physiology and pathology but also diagnostic possibilities and therapeutic advantages. Chronobiology, chronophysiology and its subspecialities,... -
Original Article : Therapeutic effect of oregano essential oil on subclinical bovine mastitis caused by Staphylococcus aureus and Escherichia coli
Byung Wook Cho , Chun Nam Cha , Soo Mi Lee , Mee Jeong Kim , Ju Yeon Park , Chang Yeol Yoo , Song Ee Son , Suk Kim , Hu 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 4호 7 253-257 (5 pages)
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Classification of acute clinical mastitis on the base of vital signs and complete blood count test in dairy cows
Tai Young Hur , Seog Jin Kang , Young Hun Jung , Hyun June Lee , Kwang Seok Ki , Chang Yong Choe , Guk Hyun Suh 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2013, 제 53권 제 1호 4 19-23 (5 pages)
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옥수수 가공형태와 RUP 수준이 전환기 젖소의 생산성에 미치는 영향
김현섭, 이종석, 김용국, 이왕식, Kim. H.S., Lee. J.S., Kim. Y.G., Lee. W.S. 한국동물자원과학회 한국동물자원과학회지 8 Pages
한국동물자원과학회 한국동물자원과학회지 2005, Vol.47 No.6 1001-1008 (8 pages)
본 연구는 젖소에서 곡류의 가공형태 및 RUP수준이 효과를 구명하기 위하여 분만 전 3주부터 분만 후 3주까지 전환기 Holstein종 경산우 20두(4처리 5반복)에게 TMR 사료를 GCR30 (파쇄옥수수+RUP 30%), GCR40 (파쇄옥수수+RUP40%), FCR30 (후레이크옥수수+RUP 30%), FCR40 (후레이크옥수수+RUP 40%)로 하여, 건유기와 비유기로 구분하여 배합하여 급여하였다. 본 연구로부터 얻어진 결과를 요약하면 다음과 같다. 분만전 사료의 건물 섭취량은 처리별로 평균 14～15kg이었으며, 건유기간 중 사료섭취량에는 유의한 차이가 없었으나, 분만... -
허브부식토의 사료내 첨가에 따른 In Vitro 발효특성과 젖소의 유생산성에 미치는 영향
김현섭, 박중국, 김홍윤, 김상범, 양승학, 김창현, 안종호, Kim. Hyeon-Shup, Park. Joong-Kook, Kim. Hong-Yun, Kim. Sang-Bum, Yang. Seung-Hak, Kim. Chang-Hyun, Ahn. Jo 한국초지조사료학회 한국초지조사료학회지 14 Pages
한국초지조사료학회 한국초지조사료학회지 2011, Vol.31 No.2 177-190 (14 pages)
본 연구는 허브 부식토를 이용하여 첨가 수준별 in vitro 반추위 발효특성 평가와 젖소를 이용하여 급여시 유생산성에 미치는 영향을 조사하기 위하여 본 연구를 수행하였다. 시험 1에서는 티머시 건초를 기질로 하여 허브부식토(herbaceous peat)를 0,1 및 5%를 3반복으로 각각 첨가하여 in vitro 반추위내 pH, 가스발생량, VFA (volatile fatty acid), ammonia-N 및 건물분해율을 조사하여 반추위내 발효성상의 변화를 평가하였다. pH 변화는 0, 3, 12 및 24시간 배양에 있어서 모든 처리구에서 유의한 차이를 보이지 않았지만 6시간.... -
Antepartal insulin-like growth factor concentrations indicating differences in the metabolic adaptive capacity of dairy cows
Marion Piechotta*, Lars Holzhausen, Marcelo Gil Araujo, Maike Heppelmann, Anja Sipka, Chistiane Pfarrer, Hans-Joachim Schuberth, 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 2 343-352 (10 pages)
Cows with different Insulin-like Growth Factor-I (IGF-I) concentrations showed comparable expression levels of hepatic growth hormone receptor (GHR). Suppressor of cytokine signaling 2 (SOCS2), could be responsible for additional inhibition of the GHR signal cascade. The aims were to monitor cows with high or low antepartal IGF-I concentrations (IGF-I or IGF-I), evaluate the interrelationships of endocrine endpoints, and measure hepatic SOCS2 expression. Dairy cows (n = 20) were selected (240 to... -
Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey
G?khan Inat*, Belgin Siriken 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 7 321-326 (6 pages)
This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected from Samsun Province of Turkey. A total of 200 samples collected from cattle carcasses and the rectal contents of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200... -
원저 : 대규모 한우 번식 목장에서의 10년간 송아지 폐사 원인
김의형, 정영훈, 최창용, 강석진, 장선식, 조상래, 양병철, 허태영 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 2호 1 75-80 (6 pages)
