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전체 선택해제
총 125건 검색
본문검색 결과만 보기
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Development of a Matrix-prespotted Plate for Enhancing the Reproducibility of Serum Glycan Analysis by MALDI-TOF-MS
Ha. Mi-Young, In. Young-Ha, Maeng. Hye-Sun, Zee. Ok-Pyo, Lee. Jong-Sik, Kim. Yang-Sun 한국질량분석학회 Mass spectrometry letters 4 Pages
한국질량분석학회 Mass spectrometry letters 2011, Vol.2 No.3 61-64 (4 pages)
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MALDI-TOF MS를 이용한 효모에서의 황화수소 생성 단백질의 동정
조현남, 판루안, 유동찬, 양선아, 이인선, 김재형, 백효현, 지광환, Cho. Hyun-Nam, Fan. Lu-An, Yoo. Dong-Chan, Yang. Seun-Ah, Lee. In-Seon, Kim. Jae-Hyung, Baek. Hyo-Hyun 한국생물공학회 한국생물공학회지 6 Pages
한국생물공학회 한국생물공학회지 2008, Vol.23 No.5 425-430 (6 pages)
후 gel에서 추출하는 방법으로 MALDI-TOFMS의 시료를 간단히 얻을 수 있었다. PMF 방법과 MS/MS ion search 분석을 통해 간편하게 효모에서 황화수소를 형성할 가능성이 있는 세가지 단백질의 동정에 성공하였다. 이 세 가지 단백질은 CYS3, CYS4, MET17 유전자의 단백질로서 cystathionine $gamma$-lyase, CBS, OASS 임이 밝혀졌다. 그리고 이 세 단백질들은 L-cysteine과 $eta$-mercaptoethanol의 존재하에서 황화수소를 실제로 생성함을 확인하였다. 본 연구에서 표적의 물질을 생산하는 단백질들을 젤상에서의 활성측정과 MALDI-TOF... -
Proteome Analysis of Drosophila melanogaster Used 2-DE and MALDI- TOF-MS
박정원, 차재영, 송재영, 김희규, 김범규, 전병삼, Park. Jeong-Won, Cha. Jae-Young, Song. Jae-Young, Kim. Hee-Kyu, Kim. Beom-Kyu, Jeon. Beong-Sam 한국생명과학회 생명과학회지 7 Pages
한국생명과학회 생명과학회지 2005, Vol.15 No.3 427-433 (7 pages)
전체 유전자의 염기서열이 보고되었으며, 유전자의 기능 분석과 발현 단백질에 대한 연구가 진행되고 있다. 본 연구에서는 야생형 초파리에서 추출한 단백질을 이차원 전기영동을 통해 400여개 이상의 단백질 스폿으로 분리하였으며, 각 스폿을 적출하여 트립신으로 처리하여 얻어진 펩타이드 단편을 MALDI-TOF-MS를 이용한 펩타이드 지문 검색으로 질량을 측정하였다. 측정된 질량을 초파리 데이터 베이스를 이용하여 분리한 단백질을 동정함으로써 59개의 유전자에서 발현되는 65개의 단백질 스폿을 동정하였다. 이러한 결과는 향후의... -
A Comprehensive Identification of Synaptic Vesicle Proteins in Rat Brains by cRPLC/MS-MS and 2DE/MALDI-TOF-MS
Lee. Won-Kyu, Kim. Hye-Jung, Min. Hye-Ki, Kang. Un-Beom, Lee. Cheol-Ju, Lee. Sang-Won, Kim. Ick-Young, Lee. Seung-Taek, Kwon. Oh-Seung, Y 대한화학회 Bulletin of the Korean Chemical Society 11 Pages
대한화학회 Bulletin of the Korean Chemical Society 2007, Vol.28 No.9 1499-1509 (11 pages)
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Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF)- Based Cloning of Enolase, ENO1, from Cryphonectria parasitica
Kim. Myoung-Ju, Chung. Hea-Jong, Park. Seung-Moon, Park. Sung-Goo, Chung. Dae-Kyun, Yang. Moon-Sik, Kim. Dae-Hyuk 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2004, Vol.14 No.3 620-627 (8 pages)
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Selective Analysis of Thiol-Containing Molecules Using Nanoengineered Micro Gold Shells and LDI-TOF MS
Lee. Jeong-Wook, Lee. Ju-Hee, Oh. Hong-Seok, Mok. Hye-Jung, Yeo. Woon-Seok 대한화학회 Bulletin of the Korean Chemical Society 3 Pages
대한화학회 Bulletin of the Korean Chemical Society 2012, Vol.33 No.9 3076-3078 (3 pages)
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Characterization of Thermal Degradation of Polytrimethylene Terephthalate by MALDI-TOF Mass Spectrometry
Jang. Sung-Woo, Yang. Eun-Kyung, Jin. Sung-Il, Cho. Young-Dal, Choe. Eun-Kyung, Park. Chan-Ryang 대한화학회 Bulletin of the Korean Chemical Society 6 Pages
대한화학회 Bulletin of the Korean Chemical Society 2012, Vol.33 No.3 833-838 (6 pages)
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Hydrolytic Degradation of Synthetic Polytrimethylene Terephthalate and Characterization by MALDI-TOF Mass Spectrometry
Yang. Eun-Kyung, Jang. Sung-Woo, Cho. Young-Dal, Choe. Eun-Kyung, Park. Chan-Ryang 대한화학회 Bulletin of the Korean Chemical Society 6 Pages
대한화학회 Bulletin of the Korean Chemical Society 2011, Vol.32 No.2 477-482 (6 pages)
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Investigation of Angiotensin Glycosylation by MALDI-TOF and ESI Tandem Mass Spectrometry
Park. Soo-Jin, Park. Deok-Hie, Sul. Soo-Hwan, Oh. Sung-Hwan F., Park. In-Sook, Chung. Doo-Soo, Kim. Hie-Joon, Kim. Min-Sik, Lee. Sang-Wo 대한화학회 Bulletin of the Korean Chemical Society 10 Pages
대한화학회 Bulletin of the Korean Chemical Society 2004, Vol.25 No.12 1791-1800 (10 pages)
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Evaluation of MALDI Biotyping for Rapid Subspecies Identification of Carbapenemase-Producing Bacteria via Protein Profiling
Somboro. Anou M., Tiwari. Dileep, Shobo. Adeola, Bester. Linda A., Kruger. Hendrik G., Govender. Thavendran, Essack. Sabiha Y. 한국질량분석학회 Mass spectrometry letters 5 Pages
한국질량분석학회 Mass spectrometry letters 2014, Vol.5 No.4 110-114 (5 pages)
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우유 및 유제품 중 미생물 동정을 위한 MALDI-TOFMS활용
김현욱, 함준상, 설국환, 한상하, 박범영, 오미화, Kim. Hyoun Wook, Ham. Jun-Sang, Seol. Kuk-Hwan, Han. Sangha, Park. Beam Young, Oh. Mi-Hwa 한국유가공기술과학회 한국유가공기술과학회지 7 Pages
한국유가공기술과학회 한국유가공기술과학회지 2012, Vol.30 No.2 131-137 (7 pages)
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Immunomodulatory Effect of Adipose Mesenchymal Stem Cell-Derived Exosomes in Lipopolysaccharide- Stimulated RAW 264.7 Cells through Indirect Contact
Jienny Lee, Mi Jeong Park, Jeong Su Byeon, Na Yeon Gu, In Soo Cho, Sang Ho Cha 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 91 134-134 (1 pages)
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Matrix-assisted laser desorption ionization-time of flight mass spectrometry based identification of Edwardsiella ictaluri isolated from Vietnamese striped catfish (Pangasius hypothalamus)
Truong Quynh Nhu, Seong Bin Park, Si Won Kim, Jung Seok Lee, Se Pyeong Im, Jassy Mary S. Lazarte, Jong Pyo Seo, Woo-Jai Lee, Jae 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 15 377-383 (7 pages)
(MALDI-TOF MS; applied using a MALDI Biotyper) to conduct rapid, easy and accurate identification of E. ictaluri. MALDI-TOF MS could directly detect the specific peptide patterns of cultured E. ictaluri colonies with high (＞ 2.0, indicating species-level identification) scores. MALDI Biotyper 3.0 software revealed that all of the strains examined in this study possessed highly similar peptide peak patterns. In addition, electrophoresis (SDS-PAGE) and subsequent immuno-blotting using a specific... -
Prevalence and risk factors for carriage of multi-drug resistant Staphylococci in healthy cats and dogs
Paola Gandolfi-Decristophoris, Gertraud Regula, Orlando Petrini, Jakob Zinsstag, Esther Schelling 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 11 449-456 (8 pages)
We investigated the distribution of commensal staphylococcal species and determined the prevalence of multi-drug resistance in healthy cats and dogs. Risk factors associated with the carriage of multi-drug resistant strains were explored. Isolates from 256 dogs and 277 cats were identified at the species level using matrix-assisted laser desorption ionisation-time of flight mass spectrometry. The diversity of coagulase-negative Staphylococci (CNS) was high, with 22 species in dogs and 24 in... -
Identification of a Marker Protein for Cardiac Ischemia and Reperfusion Injury by Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry
YoungsukLee, NariKim, HyunjuKim, HyunJoo, YoungnamKim, DaehoonJeong, DangVanCuong, EuiyongKim, DaeYoungHur, YoungShikPark, YongGeunHong, Sa 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.4 6 207-212 (6 pages)
or ischemiareperfusion injury (IR) using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). We compared 2DE gels of control (uninjured) cardiac tissue with those of IPC and IR cardiac tissue. Expression of one protein was detected in IR heart tissue, however the protein was not detected in the samples of control and IPC tissue. To further characterize the detected protein molecule, the protein in the 2D gel was isolated and... -
Proteomic Reference Map and Comparative Analysis between Streptomyces griseus S4-7 and wbiE2 Transcription Factor-Mutant Strain
Jisu Kim, Young Sang Kwon, Dong-Won Bae, Youn-Sig Kwak 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 8 185-191 (7 pages)
Streptomyces griseus S4-7, a well-characterized key- stone taxon among strawberry microbial communi- ties, shows exceptional disease-preventing ability. The whole-genome sequence, functional genes, and bioactive secondary metabolites of the strain have been described in previous studies. However, proteomics studies of not only the S4-7 strain, but also the Streptomyces genus as a whole, remain limited to date. Therefore, in the pres- ent study, we created a proteomics reference map for S.... -
Reference map of soluble proteins from Salmonella enterica serovar Enteritidis by two-dimensional electrophoresis
Park MR, Lee EG, Kim YH, Jung TS, Shin YS, Shin GW, Cha HG, Kim GS 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 2호 7 143-149 (7 pages)
(MALDI-TOF) mass spectrometry (MS) can analyze unambiguously identity of the spots from a 2-dimensional electrophoresis (2-DE) gel. This study developed a technique for 2-DE of Salmonella enterica serovar Enteritidis (S. enteritidis) by improving the dissolution conditions by 2-DE using a pH 4 - 7 immobilized pH gradient (IPG) strip. This report examines the protein components from the patterns of the S. enteritidis protein. The most abundant protein displayed a great number of clusters within... -
Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage
Ryoo. Sung-Weon, Park. Young-Kil, Park. Sue-Nie, Shim. Young-Soo, Liew. Hyun-Jeong, Kang. Seong-Man, Bai. Gill-Han 한국미생물학회 The journal of microbiology 4 Pages
한국미생물학회 The journal of microbiology 2007, Vol.45 No.3 268-271 (4 pages)
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Discrimination of Bacillus anthracis Spores by Direct in-situ Analysis of Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry
Jeong. Young-Su, Lee. Jonghee, Kim. Seong-Joo 대한화학회 Bulletin of the Korean Chemical Society 5 Pages
대한화학회 Bulletin of the Korean Chemical Society 2013, Vol.34 No.9 2635-2639 (5 pages)
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Proteome Analysis of Vernalization-Treated Arabidopsis thaliana by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Cho. Mi-Ran, Lee. Kyung-Hyeon, Hyun. You-Bong, Lee. Il-Ha, Kim. Hie-Joon 대한화학회 Bulletin of the Korean Chemical Society 5 Pages
대한화학회 Bulletin of the Korean Chemical Society 2007, Vol.28 No.3 427-431 (5 pages)
