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총 348건 검색
본문검색 결과만 보기
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Analysis of Grapevine rupestris stem pitting-associated virus in Slovakia Reveals Differences in Intra-Host Population Diversity and Naturally Occurring Recombination Events
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 1호 4 34-42 (9 pages)
their randomized distribution in the infected SK704 grapevine plant. Comparative analysis of fulllength genome sequences of four Slovak GRSPaV isolates determined in this work and 14 database sequences showed that population of the virus cluster into four major phylogenetic lineages. Moreover, our analyses suggest that genetic recombination along with point mutations could play a significant role in shaping evolutionary history of GRSPaV and contributed to its extant genetic diversification. -
Multi-Homologous Recombination-Based Gene Manipulation in the Rice Pathogen Fusarium fujikuroi
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 1 173-181 (9 pages)
Gene disruption by homologous recombination is widely used to investigate and analyze the function of genes in Fusarium fujikuroi, a fungus that causes bakanae disease and root rot symptoms in rice. To generate gene deletion constructs, the use of conventional cloning methods, which rely on restriction enzymes and ligases, has had limited success due to a lack of unique restriction enzyme sites. Although strategies that avoid the use of restriction enzymes have been employed to overcome this... -
Research Notes : Molecular Evidence of Recombination on Korean Isolates of Tomato yellow Leaf curl virus by Nucleotide Transversions and Transitions
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 12 378-384 (7 pages)
A recombination detection program 3 (RDP3) revealed two recombinations between the TYLCV Korea isolates and other TYLCV isolates [Thailand (AF206674), Iran (AJ132711), and Israel (X76319)]. TYLCV Jeju isolate was characterized by two recombination events (E1 and E2) caused by the presence of E1 in ORF V1 and C3, which may seem to be the mutations of the high nucleotide transversion and transition rate. Collectively, our results suggest that the occurrence of nucleotide transversions and... -
Targeted RNA Recombination of the Membrane Nucleocapsid Protein Genes between Mouse Hepatitis Virus Bovine Coronavirus
Gabriella Koljesar, Dong Wan Yoo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 3호 1 149-157 (9 pages)
The targeted RNA recombination was attempted to substitute the membrane (M) protein gene and part of the nucleocapsid (N) protein gene of mouse hepatitis virus with the corresponding sequences from bovine coronavirus. Using a defective interfering (DI) RNA-like cDNA construct derived from pMH54, 690 nucleotides representing the entire M gene and the 5' most 915 nucleotides of the N gene of the mouse hepatitis virus Albany 4 mutant were attempted to be replaced. Upon infection of cells with... -
Effects of Recombination on the Pathogenicity and Evolution of Pepper mottle virus
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 4호 15 417-421 (5 pages)
tentative recombination events using RDP3 and Splits Tree4 programs, respectively. Interestingly, tentative recombinants detected such as isolates 57, 134 and 217 were previously identified as severe isolates and 205135 and 205136 as differentiating isolates (Kim et al., 2009, Virus Res. 144:83-88). In addition, recombination was frequently detected in the Vb isolate, the first PepMoV isolate reported in Korea, suggesting significant involvement in the evolution of PepMoV in Korea. These initial... -
Enhanced Homologous Recombination in Fusarium verticillioides by Disruptionof FvKU70, a Gene Required for a Non-homologous End Joining Mechanism
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 1호 1 1-7 (7 pages)
the fact that homologous recombination (HR) frequency is very low (<2%). Screening for a true gene knock-out mutant is a laborious process due to a high number of ectopic integrations. In this study, we generated a F. verticillioides mutant (SF41) deleted for FvKU70, a gene directly responsible for non-homologous end-joining mechanism, with the aim of improving HR frequency. Here, we demonstrate that FvKU70 deletion does not impact key F. verticillioides phenotypes, e.g., development, secondary... -
Basal slip (0001)1/3<1120> dislocation in sapphire ($alpha$-Al$_2$O$_3$) single crystals Part I : recombination motion
Yoon. Seog-Young 한국재료학회 한국재료학회지 5 Pages
한국재료학회 한국재료학회지 2001, Vol.11 No.4 278-282 (5 pages)
사파이어($alpha$-$Al_2$$O_3$) 단결정에 있어 basal slip (0001)1/3<1120>의 부분전위의 재결합거동을 알아보기 위해 prism plane (1120)의 사파이어 재료를 사용하여 4점 곡강도 시험을 행하였다. 이 굽힘시험은 온도 $1200^{circ}C$~$1400^{circ}C$에서 그리고 응력은 90MPa, 120MPa, 150MPa에서 행하여졌다 굽힘시험 동안 basal전위가 이동하기 위해 잠복기가 필요하였다. 실험온도 범위내에서 잠복기의 활성화에너지는 5.6-6.0eV이었으며, 이 잠복기는 자체-상승운동으로 분해된 부분전위들이 재결합하는데 필요한 시간인... -
Pulsed ionization Chamber Technique for Measurement of Recombination Rate of Plasmas
Kim. Sang-Hoon 한국원자력학회 Journal of the Korean Nuclear Society 7 Pages
한국원자력학회 Journal of the Korean Nuclear Society 1974, Vol.6 No.4 253-259 (7 pages)
펄스된 이온화상자(PIC)의 출력 신호전압이 전자밀도가 $10^{13}$-$10^{17}$ m$^{-3}$인 플라즈마들에 대해 측정되었다. 이 PIC 기술치 한 응용으로서 이체 재결합 상수들이 PIC의 출력신호전압으로 측정된 전자밀도들로부터 구해졌다. 압력의 함수로서의 이 값들은 이론적인 예측과 잘 부합하며, 수치는 300$^{circ}$K에 있는 1-10기압의 $^3$He 플라즈마에 대해 5$ imes$$10^{-14}$ -3$ imes$$10^{-13}$ ㎥/sec의 범위에 있다. -
Construction of Pretense-defective Mutant of Bacillus subtilis by Homologous DNA Recombination
Lee. Jin-Tae, An. Bong-Jeun 한국식품저장유통학회 농산물저장유통학회지 4 Pages
한국식품저장유통학회 농산물저장유통학회지 2000, Vol.7 No.4 414-417 (4 pages)
단백질분해효소를 생산하지 않는 균주 B. subtilis MT-2의 염색체 DNA를 추출한 다음, B. subtilis AC819 균주에 상동성 유전자재조합을 이용하여 competent cell 형질전환을 시켰다. 얻어진 형질전환체를 B. subtilis HL-1이라고 명명하였으며, 그 표현형은 histidine 요구성, streptomycin 내성, tetracyclin 내성을 나타내면서 단백질 분해효소를 생산하지 않았다. 플라스미드 pUB110 을 이용한 B. subtilis HL-1의 protoplst 형질전환율은 B. subtilis MT-2의 형질전환율보다 높았다. 따라서 새로운 B. subtilis HL-1균주는... -
Site-Specific Recombination by the Integrase MJ1 on Mammalian Cell
김혜영, 윤보현, 장효일, Kim. Hye-Young, Yoon. Bo-Hyun, Chang. Hyo-Ihl 한국미생물생명공학회 한국미생물·생명공학회지 8 Pages
한국미생물생명공학회 한국미생물·생명공학회지 2011, Vol.39 No.4 337-344 (8 pages)
이전 연구에서, bacteriophage ${Phi}FC1$이 Enterococcus faecalis KBL703에서 UV induction을 통해 분리 동정되었으며, ${Phi}FC1$은 phage attachment site인 attP와 bacterial attachment site인 attB 사이에서 site-specific integration을 촉매하는 integrase를 가지고 있다는 것을 밝혀냈으며 이를 MJ1이라 명명하였다. 이 연구에서는 이를 바탕으로 MJ1에 의한 site-specific integration의 효율을 Escherichia coli와 NIH3T3 cell에서 확인 하기 위해 attP, attB, MJ1을 각각의 벡터에 삽입하였다. MJ1 인테그라제에 의한... -
합성 기질에 의해 형성된 Lambda Site-specific Recombination 중간 대사물의 분석
이나영, 유승구 한국미생물생명공학회 산업미생물학회지 6 Pages
한국미생물생명공학회 산업미생물학회지 1995, Vol.23 No.3 282-287 (6 pages)
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Analysis of Natural Recombination in Porcine Endogenous Retrovirus Envelope Genes
Lee. Dong-Hee, Lee. Jung-Eun, Park. Nu-Ri, Oh. Yu-Kyung, Kwon. Moo-Sik, Kim. Young-Bong 한국미생물생명공학회 Journal of microbiology and biotechnology 6 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2008, Vol.18 No.3 585-590 (6 pages)
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Characterization of Site-Specific Recombination by the Integrase MJ1 from Enterococcal Bacteriophage ${Phi}FC1$
Park. Mi-Ok, Lim. Ki-Hong, Kim. Tae-Hyung, Chang. Hyo-Ihl 한국미생물생명공학회 Journal of microbiology and biotechnology 6 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2007, Vol.17 No.2 342-347 (6 pages)
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ULTRAFAST INTERFACIAL ELECTRON TRAPPING AND RECOMBINATION IN PHOTOEXCITED COLLOIDAL CADMIUM SULFIDE
Kim. Seong-Kyu 한국광과학회 Journal of photoscience: an international journal officail organ of the Korean Society of Photoscien 6 Pages
한국광과학회 Journal of photoscience: an international journal officail organ of the Korean Society of Photoscien 1997, Vol.4 No.1 11-16 (6 pages)
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Increased Risk of Differentiated Thyroid Carcinoma with Combined Effects of Homologous Recombination Repair Gene Polymorphisms in an Iranian Population
Fayaz. Shima, Karimmirza. Maryam, Tanhaei. Shokoofeh, Fathi. Mozhde, Torbati. Peyman Mohammadi, Fard-Esfahani. Pezhman 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 5 Pages
아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 2013, Vol.14 No.11 6727-6731 (5 pages)
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Engineering lacZ Reporter Gene into an ephA8 Bacterial Artificial Chromosome Using a Highly Efficient Bacterial Recombination System
Kim. Yu-Jin, Song. Eun-Sook, Choi. Soon-Young, Park. Soo-Chul 생화학분자생물학회 Journal of biochemistry and molecular biology 6 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2007, Vol.40 No.5 656-661 (6 pages)
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PCR-mediated Recombination of the Amplification Products of the Hibiscus tiliaceus Cytosolic Glyceraldehyde-3-phosphate Dehydrogenase Gene
Wu. Linghui, Tang. Tian, Zhou. Renchao, Shi. Suhua 생화학분자생물학회 Journal of biochemistry and molecular biology 8 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2007, Vol.40 No.2 172-179 (8 pages)
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Effects of Energetic Disorder and Mobility Anisotropy on Geminate Electron-hole Recombination in the Presence of a Donor-Acceptor Heterojunction
Wojcik. Mariusz, Michalak. Przemyslaw, Tachiya. M. 대한화학회 Bulletin of the Korean Chemical Society 8 Pages
대한화학회 Bulletin of the Korean Chemical Society 2012, Vol.33 No.3 795-802 (8 pages)
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Dynamic Response of Charge Transfer and Recombination at Various Electrodes in Dye-sensitized Solar Cells Investigated Using Intensity Modulated Photocurrent and Photovoltage Spectroscopy
Kim. Gyeong-Ok, Ryu. Kwang-Sun 대한화학회 Bulletin of the Korean Chemical Society 4 Pages
대한화학회 Bulletin of the Korean Chemical Society 2012, Vol.33 No.2 469-472 (4 pages)
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Suppression of Charge Recombination Rate in Nanocrystalline SnO2 by Thin Coatings of Divalent Oxides in Dye-Sensitized Solar Cells
Lee. Chae-Hyeon, Lee. Gi-Won, Kang. Wee-Kyung, Lee. Doh-Kwon, Ko. Min-Jae, Kim. Kyoung-Kon, Park. Nam-Gyu 대한화학회 Bulletin of the Korean Chemical Society 6 Pages
대한화학회 Bulletin of the Korean Chemical Society 2010, Vol.31 No.11 3093-3098 (6 pages)
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Observation of Methyl Radical Recombination Following Photodissociation of CH3I at 266 nm by Time-Resolved Photothermal Spectroscopy
Suh. Myung-Koo, Sung. Woo-Kyung, Li. Guo-Sheng, Heo. Seong-Ung, Hwang. Hyun-Jin 대한화학회 Bulletin of the Korean Chemical Society 7 Pages
대한화학회 Bulletin of the Korean Chemical Society 2003, Vol.24 No.3 318-324 (7 pages)
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POLARITY AND ION RECOMBINATION CORRECTION FACTORS OF A THIMBLE TYPE IONIZATION CHAMBER WITH DEPTH IN WATER IN THE MEGAVOLTAGE BEAMS
Kim. Seong-Hoon, Huh. Hyun-Do, Choi. Sang-Hyun, Min. Chul-Hee, Shin. Dong-Oh, Choi. Jin-Ho 대한방사선방어학회 방사선방어학회지 6 Pages
대한방사선방어학회 방사선방어학회지 2009, Vol.34 No.2 43-48 (6 pages)
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Involvement of Brca1 in DNA Interstrand Cross-link Repair Through Homologous Recombination-independent Process
윤진호, Yun. Jean-Ho 한국생명과학회 생명과학회지 6 Pages
한국생명과학회 생명과학회지 2005, Vol.15 No.4 542-547 (6 pages)
시스플래틴이나 마이토마이신 C (MMC)와 같은 DNA 사슬간 교차결합 (interstrand cross-link ; ICL) 물질에 대해 Brca1 결손세포들이 보이는 높은 감수성은 Brca1 단백질이 세포의 ICL복구반응에 중요한 역할을 담당하고 있음을 암시하고 있다. Brca1 단백질은 재조합 의존성 또는 재조합 비의존성 경로를 통한 DNA 이중사슬 절단(double-strand break ; DSB) 복구에 필수적인 역할을 담당한다. 최근 본인이 속한 연구그룹에서 재조합 의존성 경로를 통한 세포의 ICL복구반응에 Brca1이 관여한다는 것을 밝혀 보고한바 있다. 본... -
Distribution of Brucella canis in body of dogs
Sung Il Kang, Jin Ju Lee, Mi Hye Hwang, Soon Seek Yoon, Ji Youl Jung, Sae Mi Lee, Suk Mi Kim, So Ra Sung, Hee Soo Lee, Moon Her 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 219 453-454 (2 pages)
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Genetic diversity and virulence of Streptococcussuis from diseased pigs in Korea
Ho Seong Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 218 452-453 (2 pages)
