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Targeted RNA Recombination of the Membrane Nucleocapsid Protein Genes between Mouse Hepatitis Virus Bovine Coronavirus
Gabriella Koljesar, Dong Wan Yoo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 3호 1 149-157 (9 pages)
1,137 of the N gene. This is the region immediately downstream of the replacement sequence and the start of the temperature resistant phenotype marker. The results suggest that the M and part of the N genes of bovine coronavirus may not be able to complement the function of those of mouse hepatitis virus. This study redirects our current approach of utilizing the MHV targeted RNA recombination as a means to study bovine coronavirus genetics towards the construction of an infectious cDNA clone. -
Detection of coronavirus and paramyxovirus in Korean bats
Ji Yeong Noh, Hye Kwon Kim, Yong Gun Choi, Sun Woo Yoon, Hyun Woo Lee, Dae Gwin Jeong 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 36 102-102 (1 pages)
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Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 4 350-357 (8 pages)
Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain... -
Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera
Hyojin Lee, Eun-Ju Kim, Jae-Young Song, Jeong Soo Choi, Ji Youn Lee, In-Soo Cho, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 6 307-314 (8 pages)
The specificity and sensitivity of cELISA was assessed by comparing the results of this assay to those of an immunofluorescence assay (IFA). The results of the cELISA using 416 field bovine serum samples and laboratory-immunized positive sera showed 98.1% consistency with those of the IFA. The cELISA used in this study did not show cross-reactivity with antisera against other viral cattle diseases. The cELISA presented in this study can be applied to detect antibodies against SFTSV in cattle. -
Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)
Feng-Shan Gao, Gui-Xue Hu*, Xian-zhu Xia, Yu-Wei Gao, Ya-Duo Bai, Xiao-Huan Zou 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 13 261-263 (3 pages)
that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV. -
Original Article : Serosurveillance and establishment of a reverse transcription-polymerase chain reaction assay for bovine parainfluenza virus type 5
Dong Kun Yang , Sung Suk Choi , Beom Joo Lee , Ha Hyun Kim , Hyun Ye Jo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 3호 6 185-189 (5 pages)
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Simultaneous Detection of Major Pathogens Causing Bovine Diarrhea by Multiplex Real-time PCR Panel
김원일, 조용일, 강석진, 허태영, 정영훈, 김남수, Kim. Won-Il, Cho. Yong-Il, Kang. Seog-Jin, Hur. Tai-Young, Jung. Young-Hun, Kim. Nam-Soo 한국임상수의학회 Journal of veterinary clinics 7 Pages
한국임상수의학회 Journal of veterinary clinics 2012, Vol.29 No.5 377-383 (7 pages)
송아지 설사병은 국내 축산산업에 큰 피해를 주는 중요한 질병이다. 다양한 감염성 원인체들이 송아지 설사병에 관련될 수가 있기 때문에 효과적인 치료를 위해서는 신속한 감별진단이 필수적이다. 따라서 소설사병 바이러스(BVDV), 소 코로나바이러스(BCoV), A형 소 로타바이러스(BRV), 살모넬라, $K99^+$ 대장균, Cryptosporidium parvum등의 6개의 주요 병원체들을 동시에 검출하는 두 개의 multiplex real-time PCR으로 구성된 PCR 패널을 개발하여 국내 농가에서 전북대학교 동물질병진단센터로 접수된 97개의 설사 분변을... -
소 Coronavirus 분리에 관한 연구
안재문, 유기조, 이종인 한국가축위생학회 韓國家畜衛生學會誌 9 Pages
한국가축위생학회 韓國家畜衛生學會誌 1997, Vol.20 No.2 195-203 (9 pages)
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Development of RT-PCR for the detection of Getah virus
Sung Suk Choi, Dong Kun Yang, Hyun Ye Jo, Ha Hyun Kim, Seung Heon Lee, In Soo Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 119 394-395 (2 pages)
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Recombinant Adenoviruses Expressing Glycoprotein or Nucleoprotein of Rabies Virus Induced High Neutralizing Antibody in Korean Raccoon Dogs
Dong Kun Yang, Ji young Choi, Ha Hyun Kim, Hyun Ye Jo, Sung Suk Choi, Jong Taek Kim 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 25 149-149 (1 pages)
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Sequenceanalysis of the partial Spike gene of transmissible gastroenteritis viruses isolated from Wild Boar in Korea
Kee Hwan Park, Se Eun Choe, Ra Mi Cha, Seong In Lim, Ki sun Kim, Sun Hee Kim, Sub Song, Jae hee Kim, Dong Jun An 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 230 320-320 (1 pages)
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Specific identification of Brucella abortus using coordination of SNPs
Se Chul Kim, Jin Ju Lee, So Ra Sung, Eun Ji Yum, Jin Sung Seo, Suk Chan Jung, Moon Her 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 231 261-261 (1 pages)
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A Review: Certified Animal welfare at slaughter plants
Ha Young Kim, Hye Won Lee, Hong Kook Kim, Kyeong Seon Ryu, Saem Ee Woo, Dae Sik Kim, Byeong Soo Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 107 142-143 (2 pages)
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Isolation and characterization of Korean porcine deltacoronavirus strain KNU16-07
Guehwan Jang, Seong-Hee Kim, Yoo Jin Lee, Seungjoon Kim, Du Sik Lee, Kyoung-Ki Lee, Changhee Lee 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 15 577-581 (5 pages)
Porcine deltacoronavirus (PDCoV) has emerged in several pig-raising countries and has been a causative pathogen associated with diarrheal diseases in South Korea since 2014. In the present study, we were able to isolate and cultivate a Korean PDCoV strain (KNU16-07) in cell culture and investigate its pathogenicity. PDCoV-inoculated piglets showed watery diarrhea accompanied by acute enteritis in the natural host. Sequencing analysis demonstrated the genetic stability of KNU16-07 for at least... -
Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method
Yuetao Li, Yongkun Zhao, Cuiling Wang, Xuexing Zheng, Hualei Wang, Weiwei Gai, Hongli Jin, Feihu Yan, Boning Qiu, Yuwei Gao, Nan 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 6 200-206 (7 pages)
Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study investigated and established an RVFV... -
Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin
Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 6 431-437 (7 pages)
Monoclonal anti-enrofloxacin antibody was prepared for a direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, and norfloxacin were 5.0, 8.3, 9.7, 21.7, 36.0, and 63.7 ng/mL, respectively. The lowest detectable level of ENR was 0.7 ng/mL in the prepared ELISA system. To validate the... -
Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers
Huayuan Lin, Qiqi Huang, Xiaoquan Guo, Ping Liu, Weilian Liu, Yuelong Zou, Shuliang Zhu, Guangfu Deng, Jun Kuang, Caiying Zhang, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 5 423-429 (7 pages)
To assess relationships between xanthine oxidase (XOD) and nephropathogenic infectious bronchitis virus (NIBV) infection, 240 growing layers (35 days old) were randomly divided into two groups (infected and control) of 120 chickens each. Each chicken in the control and infected group was intranasally inoculated with 0.2 mL sterile physiological saline and virus, respectively, after which serum antioxidant parameters and renal XOD mRNA expression in growing layers were evaluated at 8, 15 and 22... -
A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis
Jae-Ik Han, Dong-Woo Chang, Ki-Jeong Na 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 12 341-347 (7 pages)
Meningoencephalitis (ME) is a common inflammatory disorder of the central nervous system in dogs. Clinically, ME has both infectious and non-infectious causes. In the present study, a multiplex quantitative real-time polymerase chain reaction (mqPCR) panel was optimized for the detection of eight canine neurologic pathogens (Blastomyces dermatitidis, Cryptococcus spp., Neospora caninum, Borrelia burgdorferi, Bartonella spp., Toxoplasma gondii, Ehrlichia canis, and canine distemper virus [CDV]).... -
Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus
Lishan Lv, Xiaoming Li, Genmei Liu, Ran Li, Qiliang Liu, Huifang Shen, Wei Wang, Chunyi Xue, Yongchang Cao* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 6 209-216 (8 pages)
Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the spread of IBV, but currently available inactivated and attenuated virus vaccines have some disadvantages. We developed a chimeric virus-like particle (VLP)-based candidate vaccine for IBV protection. The chimeric VLP was composed of matrix 1 protein from avian influenza H5N1 virus and a... -
Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection
Won-Shik Kim, Chom-Kyu Chong, Hak-Yong Kim, Gyu-Cheol Lee, Wooseog Jeong, Dong-Jun An, Hye-Young Jeoung, Jae-In Lee, Young-Ki Le 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 10 91-97 (7 pages)
Feline leukemia virus (FeLV) causes a range of neoplastic and degenerative diseases in cats. To obtain a more sensitive and convenient diagnosis of the disease, we prepared monoclonal antibodies specific for the FeLV p27 to develop a rapid diagnostic test with enhanced sensitivity and specificity. Among these antibodies, we identified two clones (hybridomas 8F8B5 and 8G7D1) that specifically bound to FeLV and were very suitable for a diagnostic kit. The affinity constants for 8F8B5 and 8G7D1... -
An overview of calf diarrhea - infectious etiology, diagnosis, and intervention
Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)
or outcomes. The multifactorial nature of calf diarrhea makes this disease hard to control effectively in modern cow-calf operations. The purpose of this review is to provide a better understanding of a) the ecology and pathogenesis of well-known and potential bovine enteric pathogens implicated in calf diarrhea, b) describe diagnostic tests used to detect various enteric pathogens along with their pros and cons, and c) propose improved intervention strategies for treating calf diarrhea. -
Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus
Bock-Gie Jung, Jin-A Lee, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 4 135-141 (7 pages)
Germanium biotite (GB) is an aluminosilicate mineral containing 36 ppm germanium. The present study was conducted to better understand the effects of GB on immune responses in a mouse model, and to demonstrate the clearance effects of this mineral against Porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected pigs as an initial step towards the development of a feed supplement that would promote immune activity and help prevent diseases. In the mouse model,... -
Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine
Fang Yan*, Yujun Zhao, Yongting Hu, Jianyang Qiu, Wenxin Lei, Wenhui Ji, Xuying Li, Qian Wu, Xiumin Shi, Zhong Li 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 8 53-60 (8 pages)
The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immunized with the multivalent DNA vaccine twice and then boosted with an inactivated vaccine once. Antibody... -
Apoptosis induced in vivo by new type gosling viral enteritis virus
Shun Chen, Anchun Cheng*, Mingshu Wang*, Dekang Zhu, Renyong Jia, Qihui Luo, Hengmin Cui, Yi Zhou, Yin Wang, Zhiwen Xu, Zhengli 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 5 333-339 (7 pages)
In this study, apoptosis was induced by new type gosling viral enteritis virus (NGVEV) in experimentally infected goslings is reported in detail for the first time. After 3-day-old goslings were orally inoculated with a NGVEV- CN strain suspension, the time course of NGVEV effects on apoptotic morphological changes of the internal tissues was evaluated. These changes were observed by histological analysis with light microscopy and ultrastructural analysis with transmission electron microscopy.... -
Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection
Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Hyoung Joon Moon, Tae Yung Kim, Bong Kyun 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 14 349-352 (4 pages)
An indirect porcine epidemic diarrhea (PED)virus (PEDV)enzyme-linked immunosorbent assay (ELISA) was compared with the serum neutralization (SN)test by testing 46 samples from experimentally infected sows,73 samples from naive sows,and 1,024 field sow samples from 48 commercial swine farms of undefined PED status. The SN test and the ELISA were performed using PEDV, KPEDV-9 strain.Viral proteins as a coating antigen of PEDV ELISA were extracted from the cytoplasm of PEDV-infected Vero cells... -
Effect of soluble porcine aminopeptidase N on antibody production against porcine epidemic diarrhea virus
Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Han Sang Yoo, Yong Suk Jang, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 11 353-357 (5 pages)
of coronavirus group I which includes porcine epidemic diarrhea virus (PEDV) use porcine aminopeptidase N (pAPN) as a cellular receptor. Cellular receptors play an important role in virus attachment and entry. However, the low permissiveness of PEDV to APN-expressing porcine cell lines has made it difficult to elucidate the role of pAPN in vitro. The purpose of this study was to prove whether the treatment of soluble pAPN could enhance the antibody production against PEDV in guinea pigs, rabbits... -
Identification of a putative cellular receptor 150 kDa polypeptide for porcine epidemic diarrhea virus in porcine enterocytes
Jin Sik Oh, Dae Sub Song, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 12 269-275 (7 pages)
the coronavirus group I, human coronavirus (HCoV-229E) and transmissible gastroenteritis virus (TGEV) which uses aminopeptidase N (APN), a 150 kDa protein, as their receptors. However, the receptor of the PEDV has not been identified yet. A virus overlay protein binding assay (VOPBA) was used to identify PEDV binding protein in permissive cells. The binding ability of PEDV to porcine APN (pAPN) and the effects of pAPN on infectivity of PEDV in Vero cells were also investigated. VOPBA identified... -
Analysis for nucleotide sequence of the small membrane (sM) protein gene of porcine epidemic diarrhea virus Chinju99 isolated in Korea
SangGeonYeo, ChangHeeLee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2007, 제 47권 제 2호 5 169-174 (6 pages)
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Glycyrrhizin and Morroniside Stimulate Mucin Secretion from Cultured Airway Epithelial Cells
HoJinHeo, HyunJaeLee, CheolsuKim, KunHoSon, YoungChoongKim, YoungSikKim, SamSikKang, YangChunPark, YunHeeKim, UnKyoSeo, JeongHoSeok, Choong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2006, Vol.10 No.6 4 317-322 (6 pages)
In this study, we investigated whether glycyrrhizin, prunetin and morroniside affect mucin secretion from cultured airway epithelial cells and compared the possible activities of these agents with the inhibitory action on mucin secretion by poly-L-lysine (PLL) and the stimulatory action by adenosine triphosphate (ATP). Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using 3H-glucosamine for 24 h and chased for 30 min in the presence of varying... -
인수공통감염병의 역사와 현황
임현술 한국농촌간호학회 한국농촌간호학회 학술대회지 106 Pages
한국농촌간호학회 한국농촌간호학회 학술대회지 춘계 2 1-106 (106 pages)
