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전체 선택해제
총 440건 검색
본문검색 결과만 보기
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Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system
Wonyou Lee, Kilyoung Song, Inhyung Lee, Hyungdo Shin, Byeong Chun Lee, Seongchan Yeon, Goo Jang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 15 509-516 (8 pages)
Transvaginal ultrasound-guided follicle aspiration is one method of obtaining recipient oocytes for equine somatic cell nuclear transfer (SCNT). This study was conducted: (1) to evaluate the possibility of oocyte aspiration from pre-ovulatory follicles using a short disposable needle system (14-G) by comparing the oocyte recovery rate with that of a long double lumen needle (12-G); (2) to investigate the developmental competence of recovered oocytes after SCNT and embryo transfer. The recovery... -
Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
Marie M Meyerholz, Kirsten Mense, Michael Lietzau, Ana Kassens, Matthias Linden, Hendrike Knaack, Elisa Wirthgen, Andreas Hoefli 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 4 413-421 (9 pages)
This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The concentrations... -
Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs
Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 16 373-380 (8 pages)
This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Morphologically good (MGCOCs) and poor oocytes (MPCOCs) were untreated or treated with 1 nM rapamycin during 0–22 h, 22–42 h, or 0–42 h of IVM. Rapamycin had no significant effects on nuclear maturation and blastocyst formation... -
Factors affecting the success of resynchronization protocols with or without progesterone supplementation in dairy cows
Annette Forro , Georgios Tsousis, Nicola Beindorff, Ahmad Reza Sharifi, Christos Brozos, Heinrich Bollwein 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 16 121-126 (6 pages)
The objective of this study was to investigate factors that influence the success of resynchronization protocols for bovines with and without progesterone supplementation. Cow synchronized and not found pregnant were randomly assigned to two resynchronization protocols: ovsynch without progesterone (P4) supplementation (n = 66) or with exogenous P4 administered from Days 0 to 7 (n = 67). Progesterone levels were measured on Days 0 and 7 of these protocols as well as 4... -
Eggshell apex abnormalities associated with Mycoplasma synoviae infection in layers
Eun-Ok Jeon, Jong-Nyeo Kim, Hae-Rim Lee, Bon-Sang Koo, Kyeong-Cheol Min, Moo-Sung Han, Seung-Baek Lee, Yeon-Ji Bae, Jong-Suk Mo, 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 12 579-582 (4 pages)
Eggs exhibiting eggshell apex abnormalities (EAA) were evaluated for changes in shell characteristics such as strength, thickness, and ultrastructure. Mycoplasma synoviae (MS) infection was confirmed by serological assay along with isolation of MS from the trachea and oviduct. Changes in eggshell quality were shown to be statistically significant (p ＜ 0.01). We also identified ultrastructural changes in the mammillary knob layer by Scanning Electron Microscopy. While eggs may seem to be... -
Laparoscopic left hepatectomy in swine: a safe and feasible technique
Hua Zhang, Tao Liu, Yue Wang, Hai-feng Liu, Jian-tao Zhang, Yan-shuang Wu, Lei Lei, Hong-bin Wang* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 10 417-422 (6 pages)
of each resected lobe was 180 ± 51 g. Complete blood count as well as serum organics and enzyme levels normalized after about 2 weeks. During necropsy, adhesion of the hepatic raw surface to the gastric wall and omentum were observed. No other abnormalities were identified. This minimally invasive left hepatectomy technique in swine could serve as a useful model for investigating liver diseases and regeneration, and offer preclinical information to improve hepatobiliary surgical procedures. -
Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows
Hasina Santatriniaina Rasolomboahanginjatovo, Younès Chorfi*, Raynald Dupras, Louis Mills, Réjean Lefebvre 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 14 273-281 (9 pages)
The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at... -
Equine hyperimmune serum protects mice against Clostridium difficile spore challenge
Weiwei Yan, Kang-Soon Shin, Shih-Jon Wang, Hua Xiang, Thomas Divers, Sean McDonough, James Bowman, Anne Rowlands, Bruce Akey, Hu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 11 249-258 (10 pages)
a conventional mouse model. The data demonstrated that immunized horses generated antibodies against both toxins A and B that possessed toxin-neutralizing activity. Additionally, mice treated with the antiserum lost less weight without any sign of illness and regained weight back to a normal range more rapidly compared to the control group when challenged orally with 10 C. difficile spores 1 day after serum injection. These results indicate that intravenous delivery of hyperimmune serum can... -
Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos
Jongki Cho, Sungkeun Kang, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 8 225-231 (7 pages)
This study was conducted to investigate the expression of three genes related to early embryonic development in bovine transgenic cloned embryos. To accomplish this, development of bovine transgenic somatic cell nuclear transfer (SCNT) embryos was compared with non-transgenic embryos. Next, mRNA transcription of three specific genes (DNMT1, Hsp 70.1, and Mash2) related to early embryo development in transgenic SCNT embryos was compared between transgenic and non-transgenic SCNTs, parthenogenetic... -
Toxic effects of methylmercury, arsanilic acid and danofloxacin on the differentiation of mouse embryonic stem cells into neural cells
Seok-Jin Kang, Sang-Hee Jeong, Eun-Joo Kim, Young-Il Park, Sung-Won Park, Hyo-Sook Shin, Seong-Wan Son, Hwan-Goo Kang* 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 7 61-71 (11 pages)
This study was performed to assess the neurotoxic effects of methylmercury, arsanilic acid and danofloxacin by quantification of neural-specific proteins in vitro. Quantitation of the protein markers during 14 days of differentiation indicated that the mouse ESCs were completely differentiated into neural cells by Day 8. The cells were treated with non-cytotoxic concentrations of three chemicals during differentiation. Low levels of exposure to methylmercury decreased the expression of GABAA-R...
