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Sequence Analysis of Canine LINE-1 Elements and p53 Gene in Canine Transmissible Venereal Tumor
Young-Ki Choi and Chul-Joong Kim 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 6 285-292 (8 pages)
LINEs (long interspersed nuclear elements or long interspersed repeated DNA elements) contains two open reading frames (ORFs), ORF1 and ORF2. We analysed the ORF2 located in the 5' region to the first exon of oncogene c-myc in canine transmissible venereal tumor (TVT) cell. We also showed the transcription activation was induced by this TVT-LINE sequence using CAT assay. To identify the mutation of tumor suppressor gene, sequence analysis of p53 from TVT cell was performed. We identified the... -
Immunohistochemical Localization of Bcl-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis
Chang-Jong Moon, Yong-Duk Lee and Tae-Kyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 5 279-283 (5 pages)
We examined the localization of the anti-apoptotic molecule Bcl-2 in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis (EAE). Western blot analysis showed that Bcl-2 was constitutively expressed in normal spinal cords, and weakly increased in response to complete Freund's adjuvant(CFA) immunization. In EAE, with infiltration of inflammatory cells into spinal cords, Bcl-2 declined during the peak stage and further decreased during the recovery stage.... -
Biological Rhythm in Livestock
Piccione Giuseppe and Caola Giovanni 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 1 145-157 (13 pages)
The animal time structure is a basic fact of life, no matter if one wants to study it or not. The time-dependent, mostly rhythmic, and thus to a certain degree predictable, variations of biochemical and physiological functions and of sensitivity and resistance to many environmental agents are often quite large and offer not only new insight into animal physiology and pathology but also diagnostic possibilities and therapeutic advantages. Chronobiology, chronophysiology and its subspecialities,... -
Study on Histoplasmosis (Epizootic Lymphangitis)in Cart-Horses in Ethiopia
Gobena Ameni1 and Fasika Siyoum 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 11 135-140 (6 pages)
A cross-sectional study was conducted to determine the prevalence of Histoplasma farciminosum in 2,907 carthorses using clinical and microbiological examinations at three towns (Debre Zeit, Mojo and Nazareth), Central Ethiopia, between December 1999 and January 2001. An overall prevalence of 26.2% (762/2,907) was recorded; the highest prevalence (39.1%) being recorded at Mojo whereas the lowest (21.1%) was recorded at Nazareth. The difference in prevalence among the three towns was highly... -
Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica , Pasteurella multocida and Actinobacillus pleuropneumoniae
Jong Man Kim, Han Sang Yoo, In Soo Choi, Won Hur, Na Ri Shin 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 8 47-57 (11 pages)
Swine respiratory diseases induce severe economic losses in the swine industry worldwide. Several methods have been developed and applied to control these diseases. However, there are still problems of disease control in the swine industry. Recently, egg yolk antibodies have been found to offer several advantages for disease control in animals and humans. In a previous study (24), antibodies to several causative pathogens of swine respiratory diseases were developed. However, several problems... -
Genetic characterization of porcine circovirus - 2 field isolates from PMWS pigs
Jin H. Kim, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 6 31-39 (9 pages)
PMWS is a new emerging disease in swine herds worldwide. Field isolates of PCV-2, a putative major causative agent of PMWS, were isolated and genetically characterized. Viral genome of two field isolates (PC201DJ and PC201SS) from pigs showing typical PMWS was sequenced. The nucleotide sequence homology with other PCV-2 isolates was ranging from 95% to 99% in complete viral genomic sequence. The highly conserved nonanucleotide motif of replication origin was identical to that of other PCV-2... -
Cloning a new allele form of bovine TNF-a
Jongsam Ahn 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 12 139-141 (3 pages)
Although little is known on the function of gammadelta T lymphocytes, there is increasing evidence that gammadelta T lymphocytes are early responders and modulators of immune responses against pathogens and cytokines such as IL-2, IL-7, IL-15 and TNF-a. To study the role TNF-a on gammadelta T lymphocytes, we cloned bovine TNF-a. Sequence analysis revealed that a new allele form of bovine TNF-a was cloned which has 3 additional nucleotide sequences as well as 3 nucleotide substitutions compared... -
Melatonin ameliorates autoimmune encephalomyelitis through suppression of intercellular adhesion molecule-1
JongChul Kang, Meejung Ahn, Yong-Sik Kim, Changjong Moon, Yongduk Lee, Myung-Bok Wie, Young-jae Lee and Taekyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 3 85-89 (5 pages)
Melatonin (N-acetyl-5-methoxytryptamine), a pineal neurohormone, is a hydroxyl radical scavenger and antioxidant, and plays an important role in the immune system. We studied the effect of exogenous melatonin on the pathogenesis of experimental autoimmune encephalomyelitis (EAE). EAE was induced in Lewis rats by immunization with rat spinal cord homogenates. Subsequent oral administration of melatonin at 5 mg/kg significantly reduced the clinical severity of EAE paralysis compared with... -
Laminitis in Przewalski horses kept in a semireserve
Klaus-Dieter Budras, Klaus Scheibe, Bianca Patan, Wolf J. Streich, Kabsu Kim 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 1 1-7 (7 pages)
the three laminitic mares from the rich pasture in the main part of the semireserve and to confine them onto the poorer pasture of the small separately fenced area. All three affected mares had fully recovered from their laminitic condition. Prevention of grass laminitis can be achieved by the following measures: 1. Reduction in grass intake could be achieved by increasing the grazing pressure by an increase in stocking rate of the horses or mixed grazing with another species such as sheep. 2. A... -
Expression and characterization of the flavoprotein domain of gp91phox
Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 3 19-26 (8 pages)
Truncated forms of gp91(phox) were expressed in E. coli in which the N-terminal hydrophobic transmembrane region was replaced with a portion of the highly soluble bacterial protein thioredoxin (TRX). TRX-gp91(phox) (306-569), which contains the putative FAD and NADPH binding sites, showed NADPH-dependent NBT (nitroblue tetrazolium) reductase activity, whereas TRX-gp91(phox) (304-423) and TRX-gp91(phox) (424-569) were inactive. Activity saturated at about a 1:1 molar ratio of FAD to...


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