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391.

Research Articles : Evaluation of Potential Reference Genes for Quantitative RT-PCR Analysis in Fusarium graminearum under Different Culture Conditions

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 1 301-309 (9 pages)

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- 3,000원
The filamentous fungus Fusarium graminearum is an important cereal pathogen. Although quantitative realtime PCR (qRT-PCR) is commonly used to analyze the expression of important fungal genes, no detailed validation of reference genes for the normalization of qRT-PCR data has been performed in this fungus. Here, we evaluated 15 candidate genes as references, including those previously described as housekeeping genes and those selected from the whole transcriptome sequencing data. By a combination...
392.

Specific PCR Detection of Four Quarantine Fusarium Species in Korea

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 16 409-416 (8 pages)

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RPB2 successfully amplified specific fragments from five F. stilboides isolates grouped within a single phylogenetic clade. A specific TEF1 primer set amplified a DNA fragment from only four of the 12 F. redolens strains examined, which were grouped within a single phylogenetic clade. All of the F. semitectum var. majus isolates could be specifically detected with a single RPB2 primer set. The specificity of the primer sets developed here was confirmed using a total of 130 Fusarium isolates.
393.

Population Structure of Fusarium graminearum from Maize and Rice in 2009 in Korea

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 4 321-327 (7 pages)

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- 3,000원
We performed diagnostic PCR assays and a phylogenetic analysis using partial sequences of TEF1 (translation elongation factor-1) to determine the trichothecene chemotypes and genetic diversity of F. graminearum isolates from maize and rice samples collected in 2009 in Korea. PCR using a species-specific primer set revealed a total of 324 isolates belonging to the putative F. graminearum species complex. PCR with trichothecene chemotypespecific primers revealed that the nivalenol (NIV) chemotype...
394.

Microbial Population, Aflatoxin Contamination and Predominant Aspergillus Species in Korean Stored Rice

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 2호 4 121-129 (9 pages)

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- 3,000원
We evaluated microbial populations and aflatoxin production in unhulled and white rice from rice processing complexes of the National Agricultural Cooperative Federation in five regions in Korea and identified three predominant Aspergillus species. Fungal and bacterial populations in rice samples were significantly different between regions in 2007. Aflatoxins were also detected and varied at the levels of 2.45 - 3.43 ng per g unhulled rice grain and 1.29 - 2.09 ng per g white rice grain....
395.

Morphological and Molecular Identification of Penicillium islandicum Isolate KU101 from Stored Rice

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 14 469-473 (5 pages)

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We have previously obtained a representative isolate KU101 of the predominant Penicillium species from rice under indoor storage conditions. In this study we attempted to characterize isolate KU101 using its morphological and molecular characteristics. When the micro- and macroscopic characteristics of isolate KU101 were compared with the P. islandicum reference isolate KCCM 34763, isolate KU101 was generally identical to those of isolate KCCM 34763, however, isolate KU101 grew faster and...
396.

Purification and Phytotoxicity of Apicidins Produced by the Fusarium semitectum KCTC16676

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 7 417-422 (6 pages)

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- 3,000원
Apicidin is a cyclic tetrapeptide produced by some Fusarium species and is known to inhibit Apicomplexan histone deacetylase. The goals of this study were to determine species identity of Fusarium isolate KCTC16676, an apicidin producer, to improve a method for apicidin extraction, and to test phytotoxicity of apicidin and its analogs. We compared sequences of the translation elongation factor 1-alpha (TEF) gene in KCTC16676 with those from isolates representing diverse Fusarium species, which...
397.

Reverse Transcription Droplet Digital PCR을 활용한 Tomato Spotted Wilt Virus 검출 및 정량

이효정, 박기범, 한연수, 정래동 한국식물병리학회 식물병연구 8 Pages

한국식물병리학회 식물병연구 2021, 27권 3호 5 120-127 (8 pages)

이효정, 박기범, 한연수, 정래동 저
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- 3,000원
식물 바이러스는 작물 수확량에 상당한 손실을 일으키고 작 물 생산을 지속적으로 위협하여 세계 식량 안보에 심각한 위협 이 된다. 그 중 tomato spotted wilt virus (TSWV)는 주로 원예 작물을 감염시키는 가장 위협적인 식물 바이러스로 넓은 기주 범위를 가진다. Reverse-transcription quantitative real-time PCR (RT-qPCR)은 TSWV의 민감한 검출을 위해 널리 사용되 고 있지만 표준화의 어려움으로 인해 유용성이 감소한다. 따라 서 본 연구에서는 TSWV 검출을 위해 민감하고 정확한 reverse transcription droplet digital...
398.

The First Report of Fusarium solani Causing Wilting in Cnidium officinale in Korea

Kyeungmin Han, Hyoshin Lee, Yun Mi Park, Kwon Seok Jeon, Wonsu Choen, Chung Ryul Jung 한국식물병리학회 식물병연구 3 Pages

한국식물병리학회 식물병연구 2021, 27권 2호 5 76-78 (3 pages)

Kyeungmin Han, Hyoshin Lee, Yun Mi Park, Kwon Seok Jeon, Wonsu Choen, Chung Ryul Jung 저
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- 1,500원
Cnidium officinale is a perennial herb belonging to the family Umbelliferae. Its origin is China and is also dis- tributed in Korea and Japan. In 2017, a phenomenon of browning and wilting of leaves was discovered in the cultivation field of the National Institute of Forest Science, Suwon, Korea. The pathogens isolated from plants were consistent with Fusarium solani as a result of morphological examination and molecular phylogenetic analysis. This is the first record of F. solani on C....
399.

고랭지 여름 무에서 시들음병 발생 현황과 병원균의 병원학적 특성 연구

홍성기, 고형래, 최효원, 이영기, 김점순 한국식물병리학회 식물병연구 8 Pages

한국식물병리학회 식물병연구 2020, 26권 4호 8 256-263 (8 pages)

홍성기, 고형래, 최효원, 이영기, 김점순 저
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- 3,000원
2018년과 2019년에 강원도 여름 재배 무 포장에서 시들음병 발생이 조사되었다. 시들음병은 7월 상순 발생했고, 7월 하순과 8월 뜨거운 여름에 급속히 확산되었으며, 심한 경우, 일부 포장에서는 80%까지 발생하였다. 유묘기에 병든 무는 생육이 불량하고 뿌리 내부조직이 갈변되었다. 생육중기 동안시간이 지나면서 병든 잎은 황화되고 뿌리표면은 흰색에서흑색으로 변색되고, 도관조직은 갈변하였다. 병든 식물체로부터 23개 Fusarium균이 분리되었고, EF-1α와 IGS 염기서열 분석에 의해 Fusarium oxysporum f. sp. raphani로...
400.

Puccinia phragmitis에 의한 참소리쟁이 잎녹병 발생 보고

이승열, 박지원, 강인규, 정희영 한국식물병리학회 식물병연구 4 Pages

한국식물병리학회 식물병연구 2020, 26권 1호 8 53-56 (4 pages)

이승열, 박지원, 강인규, 정희영 저
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- 1,500원

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