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전체 선택해제
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본문검색 결과만 보기
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16S-23S rRNA Intergenic Spacer Region을 이용한 Vibrio ichthyoenteri Species-specific Primer 개발
문영건, 허문수, Moon. Young-Gun, Heo. Moon-Soo 한국미생물학회 미생물학회지 8 Pages
한국미생물학회 미생물학회지 2005, Vol.41 No.2 117-124 (8 pages)
348bp이며 tRNA gene을 가지고 있지 않았다. 이 서열을 가지고 이미 알려진 다른 Vibrio 종의 ISR 서열과 mutiple alignment를 수행한 결과 여러 영역에서 높은 가변성을 나타내어 가변 부위를 표적으로 하여 V. ichthyoenteri를 검출하기 위한 종 특이적 primer를 제작하였다. 제작된 primer의 특이성을 확인하기 위해 Vibrio 표준균주 19종의 genomic DNA와 분리균주 18 group에 genomic DNA 그리고 V. ichthyoenteri와 가장 유사한 서열을 가지고 있다고 알려진 Vibrio 종의 genomic DNA를 가지고 시험하였다. 그 결과 본 연구에서... -
Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 2 212-218 (7 pages)
of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the... -
Species-specific Detection of Erwinia pyrifoliae by PCR Assay Using Enterobacterial Repetivive Intergenic Consensus (ERIC) Primers
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 10 267-270 (4 pages)
We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens. -
Development of Streptococcus sanguinis-, Streptococcus parasanguinis-, and Streptococcus gordonii-PCR Primers Based on the Nucleotide Sequences of Species-specific DNA Probes Screened by Inverted Dot Blot Hybridization
Park. Soon-Nang, Kook. Joong-Ki 대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 7 Pages
대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 2013, Vol.38 No.2 43-49 (7 pages)
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Identification and Detection of Streptococcus anginosus Using Species-Specific 16S rDNA Primers
Cho. Ji-Sun, Yoo. So-Young, Kim. Hwa-Sook, Hwang. Ho-Keel, Min. Jeong-Beom, Kim. Byung-Hoon, Baek. Dong-Heon, Shin. Hwan-Seon, Kook. Joo 대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 4 Pages
대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 2006, Vol.31 No.1 11-14 (4 pages)
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Comparison of allele-specific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle
Kozet Avanus, Ahmet Altınel 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 9 465-470 (6 pages)
cattle. The aim of this study was to compare sensitivity, specificity, positive and negative predictive values, accuracy, and rapidity of allele-specific polymerase chain reaction (AS-PCR), created restriction-site PCR (CRS-PCR), and PCR with primer-introduced restriction analysis (PCR-PIRA), three methods used in identification of CVM carriers in a Holstein cattle population. In order to screen for the G＞T mutation in the solute carrier family 35 member A3 (SLC35A3) gene, DNA sequencing as the... -
Sensitive, Accurate PCR Assays for Detecting Harmful Dinoflagellate Cochlodinium polykrikoides Using a Specific Oligonucleotide Primer Set
Kim. Chang-Hoon, Park. Gi-Hong, Kim. Keun-Yong 한국수산과학회 Journal of fisheries science and technology 8 Pages
한국수산과학회 Journal of fisheries science and technology 2004, Vol.7 No.3 122-129 (8 pages)
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Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds
Chae-yeong Lee, Hyun-Hee Lee, Mohamed Mannaa, Nam-gyu Kim, Jung-wook Park, Ju-yun Kim, Young-Su Seo 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 4 490-498 (9 pages)
The bacteria have been reported as seed-borne pathogens from rice. Accurate detection of both pathogens on the seeds is very important for limiting the disease dissemination. Novel primer pairs targeting specific molecular markers were developed for the robust detection of B. glumae and B. gladioli. The designed primers were specific in detecting the target species with no apparent crossreactions with other related Burkholderia species at the expected product size. Both primer pairs displayed a... -
Occurrence of the Root-Knot Nematode species in Vegetable Crops in Souss Region of Morocco
Soukaina Janati, Abdellah Houari, Ahmed Wifaya, Adil Essarioui, Abdelaziz Mimouni, Abderrahim Hormatallah, Mohamed Sbaghi, Abdelfattah 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 4호 5 308-315 (8 pages)
as molecularlly using species-specific primers. The obtained results indicated that Meloidogyne javanica and M. incognita were identified in 86.4% and 13.5% of the total populations, respectively. The lowest incidence of root-knot nematodes (64%) was found in Toussous, whereas the highest frequencies of 100% and 90% were detected in Taddart and Biogra, respectively. As the majority of the samples have been infested with Meloidogyne species; this indicates that there is an urgent need to provide...
