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- JOURNAL OF VETERINARY SCIENCE(9)
- THE PLANT PATHOLOGY JOURNAL (6)
- THE KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY(2)
- 발생과 생식(2)
- FISHERIES AND AQUATIC SCIENCES(1)
- JOURNAL OF PHOTOSCIENCE: AN INTERNATIONAL JOURNAL OFFICAIL ORGAN OF THE KOREAN SOCIETY OF PHOTOSCIEN(1)
- KOREAN JOURNAL OF ICHTHYOLOGY(1)
- KOREAN JOURNAL OF VETERINARY RESEARCH(구 대한수의학회지)(1)
- 생명과학회지(1)
- 식물병연구(1)
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Determination of the Temperature-Dependent Index of Mitotic Interval (${ au}_o$) for Chromosome Manipulation in Far Eastern Catfish Silurus asotus
박인석, 임재현, Park. In-Seok, Im. Jae-Hyun 한국어류학회 Korean journal of Ichthyology 4 Pages
한국어류학회 Korean journal of Ichthyology 2001, Vol.13 No.1 85-88 (4 pages)
메기 Silurus asotus에서 염색체조작을 효과적으로 하기 위하여, 여러 온도 조건에서의 난할율과 제2, 제3 난할 시간의 평균화에 의한 체세포분열 간격지수 (${ au}_o$)를 조사하였다. 빠른 난 발생과 비동시적 발생은 고온에서 더욱 뚜렷하게 나타났다. 메기의 체세포분열 간격지수는 $22^{circ}C$에서 $21.5{pm}1.4$분, $24^{circ}C$에서 $18.5{pm}1.2$분 그리고 $26^{circ}C$에서 $14.0{pm}2.1$분이었다. 3가지 수온조건과 ${ au}_o$ 간에는 Y=-1.85X+21.9 ($R^2=0.9868$, Y는 ${ au}_o$ 그리고 X는 수온)의 직선회귀가 성립되었다. -
Temperature-dependent Index of Mitotic Interval (τ0) for Chromosome Manipulation in Korean Rose Bitterling Rhodeus uyekii
Kim. Bong-Seok, Lim. Sang-Gu, Gil. Hyun-Woo, Park. In-Seok 한국수산과학회 Fisheries and aquatic sciences 5 Pages
한국수산과학회 Fisheries and aquatic sciences 2011, Vol.14 No.4 429-433 (5 pages)
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Development of Simultaneous YAC Manipulation-Amplification (SYMA) system by Chromosome Splitting Technique Harboring Copy Number Amplification System
김연희, 남수완, Kim. Yeon-Hee, Nam. Soo-Wan 한국생명과학회 생명과학회지 5 Pages
한국생명과학회 생명과학회지 2010, Vol.20 No.5 789-793 (5 pages)
본 연구에서는 YAC manipulation system에 복제수 증폭시스템(copy number amplification system)을 도입한 Simultaneous YAC Manipulation-Amplification (SYMA) system을 구축하였다. 식물염색체를 가진 YAC clone의 splitting과 증폭을 위해 conditional centromere와 thymidine kinase (TK) 유전자를 가진 pBGTK plasmid를 구축하였고, splitting fragment의 PCR을 위한 주형으로 사용하였다. 590 kb의 YAC clone은 splitting과 동시에 copy number amplification element를 가진 100 kb YAC와 490 kb YAC로 분리되었고, 100 kb YAC는... -
Application of Chromosome Manipulation, DOP-PCR and AFLP Methods to Isolate Sex-Specific DNAs from Rumex acetosa L.
Jin. Dong-Chung, Kim. Joong-Soon, Park. ji-Young, Bong. Jae-Wook, Hur. Yoon-Kang 한국광과학회 Journal of photoscience: an international journal officail organ of the Korean Society of Photoscien 8 Pages
한국광과학회 Journal of photoscience: an international journal officail organ of the Korean Society of Photoscien 2005, Vol.12 No.2 75-82 (8 pages)
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Multi-Homologous Recombination-Based Gene Manipulation in the Rice Pathogen Fusarium fujikuroi
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 1 173-181 (9 pages)
Gene disruption by homologous recombination is widely used to investigate and analyze the function of genes in Fusarium fujikuroi, a fungus that causes bakanae disease and root rot symptoms in rice. To generate gene deletion constructs, the use of conventional cloning methods, which rely on restriction enzymes and ligases, has had limited success due to a lack of unique restriction enzyme sites. Although strategies that avoid the use of restriction enzymes have been employed to overcome this... -
Bovine adenovirus type 3 virions cannot be rescued in vivo after full-length viral genome transfection in the absence of detectable polypeptide IX
Peng Zhang, Qinghong Xue, Jing Ma, Jingjing Ren, Shuili Xia, Lu Zhang, Wenbin Wang, Suresh K. Tikoo, Enqi Du 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 12 217-227 (11 pages)
Bovine adenovirus type 3 (BAdV3) is being used in the development of potential vehicles for gene therapy and vectored vaccine. To that end, a more comprehensive description of BAdV3 biology is essential. In this study, we focused on the role of pIX in BAdV3 virion rescue after full-length BAdV3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions, which suggested that pIX was essential for the rescue of BAdV3 containing a... -
Transgenesis for pig models
Soo-Young Yum, Ki-Young Yoon, Choong-Il Lee, Byeong-Chun Lee, Goo Jang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 1 261-268 (8 pages)
Animal models, particularly pigs, have come to play an important role in translational biomedical research. There have been many pig models with genetically modifications via somatic cell nuclear transfer (SCNT). However, because most transgenic pigs have been produced by random integration to date, the necessity for more exact gene-mutated models using recombinase based conditional gene expression like mice has been raised. Currently, advanced genome-editing technologies enable us to generate... -
Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system
Wonyou Lee, Kilyoung Song, Inhyung Lee, Hyungdo Shin, Byeong Chun Lee, Seongchan Yeon, Goo Jang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 15 509-516 (8 pages)
Transvaginal ultrasound-guided follicle aspiration is one method of obtaining recipient oocytes for equine somatic cell nuclear transfer (SCNT). This study was conducted: (1) to evaluate the possibility of oocyte aspiration from pre-ovulatory follicles using a short disposable needle system (14-G) by comparing the oocyte recovery rate with that of a long double lumen needle (12-G); (2) to investigate the developmental competence of recovered oocytes after SCNT and embryo transfer. The recovery... -
Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs
Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 16 373-380 (8 pages)
This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Morphologically good (MGCOCs) and poor oocytes (MPCOCs) were untreated or treated with 1 nM rapamycin during 0–22 h, 22–42 h, or 0–42 h of IVM. Rapamycin had no significant effects on nuclear maturation and blastocyst formation...
