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101.

Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)

Geewook Shin, Hyungjun Lee, K. J. Palaksha, Youngrim Kim, Eunyoung Lee, Yongseung Shin, Eunggoo Lee, Kyungdae Park, Taesung Jung 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 14 293-295 (3 pages)

Geewook Shin, Hyungjun Lee, K. J. Palaksha, Youngrim Kim, Eunyoung Lee, Yongseung Shin, Eunggoo Lee, Kyungdae Park, Taesung Jung 저
...
- 1,500원
The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG affinity columns. These three different ligands were found to possess high affinity for black rockfish serum Ig. All of the Igs purified eluted at only 0.46 M NaCl concentration in anion exchange column chromatography and consisted of two bands at 70 kDa and 25 kDa in SDS-PAGE;...
102.

Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine

Dong-Kun Yang, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Kyung-Woo Lee, Cheong-Up Choi, Chang-Hee Kweon 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 10 271-275 (5 pages)

Dong-Kun Yang, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Kyung-Woo Lee, Cheong-Up Choi, Chang-Hee Kweon 저
...
- 1,500원
The Japanese encephalitis virus (JEV) is one of causative agents of reproductive failure in pregnant sows. An indirect enzyme-linked immunosorbent assay (I-ELISA) was examined for its potential use in the rapid monitoring of the JEV, and the results were compared with those from the hemagglutination inhibition (HI) and serum neutralization (SN) tests. The comparative analysis showed that the results of I-ELISA showed a significant correlation with the conventional HI (r = 0.867) and SN tests (r...
103.

Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus

Charles I. Muleke, Yan Ruofeng, Xu Lixin, Sun Yanming, Li Xiangrui 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 7 249-255 (7 pages)

Charles I. Muleke, Yan Ruofeng, Xu Lixin, Sun Yanming, Li Xiangrui 저
...
- 3,000원
of the cysteine protease class of proteins. In the acidic pH range, the isolated protein actively degraded hemoglobin (Hb), the heavy chain of goat immunoglobulin G, and azocasein. By contrast, it degraded fibrinogen in the alkaline pH range. These activities were strongly inhibited in the presence of the cysteine protease inhibitor E-64. While the protein digested Hb, it did not induce the agglutination of erythrocytes from its natural host. These results suggest that the HC58 protein may play...
104.

Modulatory effects of chitosan adipate on the T and B lymphocyte subsets in mice

Bozena Obminska-Mrukowicz, Marianna Szczypka, Bartosz Gaweda 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 11 157-160 (4 pages)

Bozena Obminska-Mrukowicz, Marianna Szczypka, Bartosz Gaweda 저
...
- 1,500원
This study examined the subsets of T lymphocytes in the thymus, spleen and mesenteric lymph nodes as well as the subsets of B lymphocytes in the spleen and mesenteric lymph nodes in mice administered chitosan adipate (20 mg/kg) intraperitoneally once or four times at 24 h intervals. The results showed that chitosan adipate decreased the percentage of immature CD4+CD8+ thymic T cells and increased the percentage of mature CD4+ and CD8+ thymocytes. The most significant stimulating effect was...
105.

Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan

Muhammad Wasif Malik, Najma Ayub, Irfan Zia Qureshi 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 7 43-46 (4 pages)

Muhammad Wasif Malik, Najma Ayub, Irfan Zia Qureshi 저
...
- 1,500원
Infectious bursal disease (IBD)is an acute and highly contagious disease of young chickens caused by Birnavirus. Mortality of infected birds can be best prevented if injected with antibodies.The present study was an attempt to raise specific hyper-immune polyclonal antibodies against IBD virus in Pakistan.Commercial layers divided into four groups were injected with IBD vaccine subcutaneously according to four different treatment regimens.Eggs were collected daily and antibodies were purified...
106.

CT myelography of the thoraco-lumbar spine in 8 dogs with degenerative myelopathy

Jeryl C. Jones, Karen D. Inzana, John H. Rossmeisl, Robert L. Bergman, Tana Wells, Katherine Butler 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 13 341-348 (8 pages)

Jeryl C. Jones, Karen D. Inzana, John H. Rossmeisl, Robert L. Bergman, Tana Wells, Katherine Butler 저
...
- 3,000원
CT myelography of the T11-L2 region was performed in 8 large-breed dogs with a clinical diagnosis of degenerative myelopathy (DM) and 3 large-breed dogs that were clinically normal. CT myelographic characteristics were recorded for each dog, at each disc level. Area measurements of the spinal cord, dural sac, vertebral canal, and vertebral body were recorded at 4 slice locations for each disc level. Mean area ratios were calculated and graphically compared, by slice location and group. In all...
107.

A predictive model for the level of sIgA based on IgG levels following the oral administration of antigens expressed in Sacchromyces cerevisiae

Sung Jae Shin, Seung Won Shin, Eun Jin Choi, Deog Yong Lee, Jeong Min Ahn, Moon Sik Yang, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 8 305-309 (5 pages)

Sung Jae Shin, Seung Won Shin, Eun Jin Choi, Deog Yong Lee, Jeong Min Ahn, Moon Sik Yang, Yong Suk Jang, Han Sang Yoo 저
...
- 1,500원
is the major immunoglobulin type generally detected in the secretions of experimental animals following an effective oral immunization. Actinobacillus pleuropneumoniae causing disease in the lung of pig initially interacts, colonizes, and infects the host tissues at the mucosal surface of the respiratory tract. Also, importantly for A. pleuropneumoniae protection, the quantity of sIgA in the lung had merits associated with the mucosal immunity. However, there is no simple method to monitor the...
108.

Expression of tyrosine kinase A in the cerebral cortex of postnatal developing rat

Hyo Jung Kwon, Kyoung Youl Lee, Il Kwon Park, Mi Sun Park, Mi Young Lee, Moo Kang Kim 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 2 185-189 (5 pages)

Hyo Jung Kwon, Kyoung Youl Lee, Il Kwon Park, Mi Sun Park, Mi Young Lee, Moo Kang Kim 저
...
- 1,500원
Tyrosine kinase A (TrkA)is an essential component of the high affinity nerve growth factor (NGF)receptor necessary to the mediate the biological effects of the neurotrophins, NGF. This study examined the distribution of TrkA-immunoreactivity (IR)cells in the postnatal rat cerebral cortex and the changes that occur in postnatal development as a result of the expression of this protein. TrkA-IR was detected at postnatal day (PD)3, PD6, PD9 and PD15. Base upon their somatodendritic morphology, the...
109.

Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes

Elizabeth C. Molina 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 7 135-139 (5 pages)

Elizabeth C. Molina 저
...
- 1,500원
This study investigated the presence of cytokines interferon (IFN)-gamma, interleukins (IL) -6 and -8 in serum of cattle and buffaloes infected with Fasciola gigantica from one to 16 weeks post-infection to determine their T cell response during infection. The concentration of these cytokines was determined by sandwich enzyme-linked immunosorbent assay (ELISA). No IFN-gamma was detected in these animals while IL-6 was elevated from one to 16 weeks postinfection. Levels of IL-8 were also elevated...
110.

Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice

Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 6 125-133 (9 pages)

Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 저
...
- 3,000원
groups. The complete protection was shown in prME and live vaccine inoculated groups, respectively. We also measured neutralizing antibody and three immunoglobulin subtypes of IgG1, IgG2a and IgG2b in the sera of mice before and after challenge. Titers of IgG1 antibodies were approximately two to three times higher than that of IgG2b antibodies in all the immunized groups as compared to the control group. However, IgG2a antibody level somewhat increased after challenge, indicating T-helper type...

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