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111.

Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies

Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 6 41-46 (6 pages)

Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 저
...
- 3,000원
Five monoclonal antibodies (MAbs) and chicken immunoglobulin (IgY) were developed by immunizing with flagella purified from Listeria monocytogenes 4b and the five MAbs have been confirmed to be specific against three different epitopes of flagellin. The antibodies showed specific reaction to Listeria genus and no cross-reactivity with other bacteria tested in this experiment including E.coli O157:H7 and Salmonella enteritidis. Sandwich enzyme-linked immunosorbent assays (ELISA) using the MAbs...
112.

Experimental evaluation of pathogenicity of Lactococcus garvieae in black rockfish (Sebastes schlegeli)

Sung-hyun Kang, Gee-wook Shin, Yong-seung Shin, Palaksha K. J., Young-rim Kim, Hyang-hee Yang, Eun-young Lee, Eung-goo Lee, Nam- 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 15 387-390 (4 pages)

Sung-hyun Kang, Gee-wook Shin, Yong-seung Shin, Palaksha K. J., Young-rim Kim, Hyang-hee Yang, Eun-young Lee, Eung-goo Lee, Nam- 저
...
- 1,500원
Black rockfish (Sebastes schlegeli) is an important mariculture species in Korea. The production of this fish is drastically declined due to bacterial diseases, particularly streptococcosis caused by Lactococcus garvieae. The bacterial surface characteristics of SJ7 and TY6 were found to have capsule but not NB13 and YS18. The experiential evaluation of L. garvieae pathogenicity, the capsular isolates showed high cumulative mortality i.e. SJ7 (100%) and TY6 (60%) compared to non-capsular...
113.

Effect of soluble porcine aminopeptidase N on antibody production against porcine epidemic diarrhea virus

Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Han Sang Yoo, Yong Suk Jang, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 11 353-357 (5 pages)

Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Han Sang Yoo, Yong Suk Jang, Bong Kyun Park 저
...
- 1,500원
A few members of coronavirus group I which includes porcine epidemic diarrhea virus (PEDV) use porcine aminopeptidase N (pAPN) as a cellular receptor. Cellular receptors play an important role in virus attachment and entry. However, the low permissiveness of PEDV to APN-expressing porcine cell lines has made it difficult to elucidate the role of pAPN in vitro. The purpose of this study was to prove whether the treatment of soluble pAPN could enhance the antibody production against PEDV in guinea...
114.

Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application

Deog Yong Lee, Young Wook Cho, Sang Gyun Kang, Sung Jae Shin, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 9 337-343 (7 pages)

Deog Yong Lee, Young Wook Cho, Sang Gyun Kang, Sung Jae Shin, Han Sang Yoo 저
...
- 3,000원
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and accurate quantification method previously used with ovine, rat, and human IL-6 proteins. However, it has never been reported to quantify porcine IL-6 protein using capture ELISA. In this study, we generated and characterized a set of IgY and mono-specific polyclonal antibodies to recombinant porcine IL-6 (rpIL-6),...
115.

Application of biotechnological tools for coccidia vaccine development

Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 1 279-288 (10 pages)

Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 저
...
- 3,000원
Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and...
116.

Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRγ-knockout mice following oral challenge with Strongyloides venezuelensis

Denis Nnabuike Onah, Yukifumi Nawa 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 6 221-226 (6 pages)

Denis Nnabuike Onah, Yukifumi Nawa 저
...
- 3,000원
Mucosal mast cell-derived chondroitin sulphates (sulphated proteoglycans) were assayed in gut washings and homogenate of FcRγ-knockout (KO) and wild-type (WT) C57BL/6 mice challenged with Strongyloides venezuelensis in order to assess their possible role in secondary immunity against enteric nematodes. Groups of immune KO and WT mice were challenged by oral gavage with 300 infective larvae (L3 ). Establishment of infection was assessed by daily faecal analysis to determine the number of eggs...
117.

Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp. Paratuberculosis

Sung Jae Shin, Han Sang Yoo, Sean P. McDonough, Yung-Fu Chang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 4 111-117 (7 pages)

Sung Jae Shin, Han Sang Yoo, Sean P. McDonough, Yung-Fu Chang 저
...
- 3,000원
Eighty-five complex (85A, 85B and 85C), 35-kDa and superoxide dismutase (SOD) were cloned, expressed and purified as antigens in an enzyme-linked immunosorbent assay (ELISA) to compare the serological reactivity of cows with different shedding levels of Mycobacterium avium subsp. paratuberculosis (MPT). Antibody responses to all recombinant antigens positively increased depending on shedding levels. In particular, antibody responses to the 35 kDa were higher than those to the others in all...
118.

Comparative studies on pheno- and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany

Siti Isrina Oktavia Salasia, Zaini Khusnan, Christoph Lammler, Michael Zschock 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 3 103-109 (7 pages)

Siti Isrina Oktavia Salasia, Zaini Khusnan, Christoph Lammler, Michael Zschock 저
...
- 3,000원
aureus isolates harboured the genes clfA and coa encoding staphylococcal clumping factor and coagulase, and the gene segments encoding the immunoglobulin G binding region and the X-region of protein A gene spa. By PCR amplification, the genes seb, seg, seh, and sei was observed for the S. aureus cultures isolated in Central Java, Indonesia and the genes sec, sed, seg, seh, sei, sej and tst for the S. aureus cultures isolated in Hesse, Germany. None of the S. aureus of both origins harboured the...
119.

Characterization of Antigenic Sites on the Rinderpest Virus N Protein Using Monoclonal Antibodies

Kang-seuk Choi, Jin-ju Nah, Young-joon Ko, Cheong-up Choi, Jae-hong Kim, Shien-young Kang, Yi-seok Joo 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 9 57-65 (9 pages)

Kang-seuk Choi, Jin-ju Nah, Young-joon Ko, Cheong-up Choi, Jae-hong Kim, Shien-young Kang, Yi-seok Joo 저
...
- 3,000원
The N protein of the rinderpest virus (RPV) was analyzed topologically and antigenically by using anti-N monoclonal antibodies (Mabs). Ten Mabs were raised against the N protein of the RPV. At least six non-overlapping antigenic sites (sites A-F) were delineated by competitive binding assays using biotinylated Mabs. Of them 5 sites (A, C, D, E and F) on the N protein were recognized by RPV-specific Mabs in ELISA and IFA while site B was recognized by Mabs reacting with both RPV and PPRV. Non-...
120.

The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)

W. C. Davis, A. M. Khalid, M. J. Hamilton, J. S. Ahn, Y. H. Park, G. H. Cantor 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 6 103-109 (7 pages)

W. C. Davis, A. M. Khalid, M. J. Hamilton, J. S. Ahn, Y. H. Park, G. H. Cantor 저
...
- 3,000원
One of the major difficulties in studying the mechanisms of host defense in economically important species indigenous to Asia and the Middle East is the lack of monoclonal antibody (mAb) reagents that define the immune systems of species other than cattle, goats, sheep, and pigs. One strategy that could obviate this problem at minimal cost is to identify existing mAbs that recognize conserved epitopes on orthologous major histocompatibility complex (MHC) and leukocyte differentiation molecules....

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