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121.

Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification

Yubao Zhang, Zhongkui Xie, John D Fletcher, Yajun Wang, Ruoyu Wang, Zhihong Guo, Yuhui He 한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 7 76-86 (11 pages)

Yubao Zhang, Zhongkui Xie, John D Fletcher, Yajun Wang, Ruoyu Wang, Zhihong Guo, Yuhui He 저
...
- 4,000원
Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse...
122.

Ralstonia solanacearum Type III Effectors with Predicted Nuclear Localization Signal Localize to Various Cell Compartments and Modulate Immune Responses in Nicotiana spp.

Hyelim Jeon, Wanhui Kim, Boyoung Kim, Sookyeong Lee, Jay Jayaraman, Gayoung Jung, Sera Choi, Kee Hoon Sohn, C?cile Segonzac 한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 4 43-53 (11 pages)

Hyelim Jeon, Wanhui Kim, Boyoung Kim, Sookyeong Lee, Jay Jayaraman, Gayoung Jung, Sera Choi, Kee Hoon Sohn, C?cile Segonzac 저
...
- 4,000원
Ralstonia solanacearum (Rso) is a causal agent of bacterial wilt in Solanaceae crops worldwide including Republic of Korea. Rso virulence predominantly relies on type III secreted effectors (T3Es). However, only a handful of Rso T3Es have been characterized. In this study, we investigated subcellular localization of and manipulation of plant immunity by 8 Rso T3Es predicted to harbor a nuclear localization signal (NLS). While 2 of these T3Es elicited cell death in both Nicotiana benthamiana and...
123.

Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose Pathogen Colletotrichum scovillei to Develop an Applied Genomics Approach

Jong-Hwan Shin, Joon-Hee Han, Hyun-Hoo Park, Teng Fu, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 4 575-584 (10 pages)

Jong-Hwan Shin, Joon-Hee Han, Hyun-Hoo Park, Teng Fu, Kyoung Su Kim 저
...
- 3,000원
transformation of KC05 using 19 different loci found throughout 10 (of 27) scaffolds, covering approximately 84.1% of the entire genome. PCR screening showed that the average transformation efficiency was about 17.1% per 100 colonies. Southern blot analyses revealed that at least one transformant per locus had single copy integration of PCR-screened positive transformants. Our results provide valuable information for a functional genomics approach to the pepper anthracnose pathogen C. scovillei.
124.

vfr, A Global Regulatory Gene, is Required for Pyrrolnitrin but not for Phenazine-1-carboxylic Acid Biosynthesis in Pseudomonas chlororaphis G05

Xia Wu, Xiaoyan Chi, Yanhua Wang, Kailu Zhang, Le Kai, Qiuning He, Jinxiu Tang, Kewen Wang, Longshuo Sun, Xiuying Hao, Weihai Xie, Yihe Ge 한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 4호 7 351-361 (11 pages)

Xia Wu, Xiaoyan Chi, Yanhua Wang, Kailu Zhang, Le Kai, Qiuning He, Jinxiu Tang, Kewen Wang, Longshuo Sun, Xiuying Hao, Weihai Xie, Yihe Ge 저
...
- 4,000원
In our previous study, pyrrolnitrin produced in Pseudomonas chlororaphis G05 plays more critical role in suppression of mycelial growth of some fungal pathogens that cause plant diseases in agriculture. Although some regulators for pyrrolnitrin biosynthesis were identified, the pyrrolnitrin regulation pathway was not fully constructed. During our screening novel regulator candidates, we obtained a white conjugant G05W02 while transposon mutagenesis was carried out between a fusion mutant...
125.

DevelDevelopment of a Quantitative Real-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid

Seong Heo, Hyun Ran Kim, Hee Jae Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 8 164-171 (8 pages)

Seong Heo, Hyun Ran Kim, Hee Jae Lee 저
...
- 3,000원
of the starting ASSVd RNA copy number of 10-fold dilutions each. Compared to the established RT-PCR methods, our method was more sensitive for detecting ASSVd. The real-time quantitative NASBA method will be fast, sensitive, and reliable for routine diagnosis and selection of viroid-free stock materials. Furthermore, real-time quantitative NASBA may be especially useful for detecting low levels in apple trees with early viroid-infection stage and for monitoring the influence on tree growth.
126.

Factors Affecting the Vineyard Populational Diversity of Plasmopara viticola

Susana Boso, Pilar Gago, Jos?-Luis Santiago, Mar?a de la Fuente, Mar?a-Carmen Mart?nez 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 4 125-136 (12 pages)

Susana Boso, Pilar Gago, Jos?-Luis Santiago, Mar?a de la Fuente, Mar?a-Carmen Mart?nez 저
...
- 4,100원
A number of authors have suggested different genetic populations of this fungus exist in Europe, each showing a different degree of virulence. Work performed to date indicates this diversity to be the result of different factors. In areas where gene flow is greater and recombination more frequent, the diversity of P. viticola appears to be wider. In vineyards isolated by geographic barriers, a race may become dominant and produce clonal epidemics driven by asexual reproduction. The aim of the...
127.

Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers

Juan C. Ochoa, Mariana Herrera, M?nica Navia, Hern?n Mauricio Romero 한국식물병리학회 The Plant Pathology Journal 13 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 3 19-31 (13 pages)

Juan C. Ochoa, Mariana Herrera, M?nica Navia, Hern?n Mauricio Romero 저
...
- 4,200원
Bud rot (BR) is the most devastating disease affecting oil palm (Elaeis guineensis) crops in Colombia. Its causal agent, Phytophthora palmivora, initiates the infection in immature oil palm leaflets producing necrotic lesions, followed by colonization of opportunistic necrotrophs, which increases disease damage. To improve the characterization of the disease, we transformed P. palmivora using Agrobacterium tumefaciens-mediated transformation (ATMT) to include the fluorescent proteins CFPSKL...
128.

Selection of Reference Genes for Real-time Quantitative PCR Normalization in the Process of Gaeumannomyces graminis var. tritici Infecting Wheat

Li-hua Xie, Xin Quan, Jie Zhang, Yan-yan Yang, Run-hong Sun, Ming-cong Xia, Bao-guo Xue, Chao Wu, Xiao-yun Han, Ya-nan Xue, Li-rong Yang 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 2 11-18 (8 pages)

Li-hua Xie, Xin Quan, Jie Zhang, Yan-yan Yang, Run-hong Sun, Ming-cong Xia, Bao-guo Xue, Chao Wu, Xiao-yun Han, Ya-nan Xue, Li-rong Yang 저
...
- 3,000원
Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression...
129.

A Small GTPase RHO2 Plays an Important Role in Pre-infection Development in the Rice Blast Pathogen Magnaporthe oryzae

Teng Fu, Joon-Oh Kim, Joon-Hee Han, Adiyantara Gumilang, Yong-Hwan Lee, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 2 470-479 (10 pages)

Teng Fu, Joon-Oh Kim, Joon-Hee Han, Adiyantara Gumilang, Yong-Hwan Lee, Kyoung Su Kim 저
...
- 3,000원
The rice blast pathogen Magnaporthe oryzae is a global threat to rice production. Here we characterized RHO2 gene (MGG_02457) that belongs to the Rho GTPase family, using a deletion mutant. This mutant ΔMorho2 exhibited no defects in conidiation and germination but developed only 6% of appressoria in response to a hydrophobic surface when compared to the wild-type progenitor. This result indicates that MoRHO2 plays a role in appressorium development. Furthermore, exogenous cAMP treatment on the...
130.

Characterization of the Lytic Bacteriophage phiEaP-8 Effective against Both Erwinia amylovora and Erwinia pyrifoliae Causing Severe Diseases in Apple and Pear

Jungkum Park, Gyu Min Lee, Donghyuk Kim, Duck Hwan Park, Chang-Sik Oh 한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 5호 10 445-450 (6 pages)

Jungkum Park, Gyu Min Lee, Donghyuk Kim, Duck Hwan Park, Chang-Sik Oh 저
...
- 3,000원
Bacteriophages, bacteria-infecting viruses, have been recently reconsidered as a biological control tool for preventing bacterial pathogens. Erwinia amylovora and E. pyrifoliae cause fire blight and black shoot blight disease in apple and pear, respectively. In this study, the bacteriophage phiEaP-8 was isolated from apple orchard soil and could efficiently and specifically kill both E. amylovora and E. pyrifoliae. This bacteriophage belongs to the Podoviridae family. Whole genome analysis...

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