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전체 선택해제
총 181건 검색
본문검색 결과만 보기
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Molecular Analysis of Botrytis cinerea Causing Ginseng Grey Mold Resistant to Carbendazim and the Mixture of Carbendazin Plus Diethofencarb
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 4호 3 322-327 (6 pages)
change of glutamate to alanine at amino acid position 198. Furthermore, the substitution of valine for glutamate led the resistance to carbendazim and the mixture at the same position of amino acid. PCR-restriction fragment length polymorphism (PCR-RFLP) analysis using the restriction endonuclease, Tsp45I and BstUI allowed differentiation of the PCR fragment of β-tubulin gene of CarSMixS isolates from that of CarRMixR and CarRMixS isolates. This method will aid in a fast detection of resistance... -
Mixed Infection of 16S rDNA I and V Groups of Phytoplasma in a Single Jujube Tree
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 1호 4 21-25 (5 pages)
A 1.2 kb fragment of the 16S rDNA from jujube phytoplasma was generated by R16F2n/ R16R2 primer pair from earlier amplified P1/P7 PCR products of cloned jujube witches` broom phytoplasmas. Enzymatic restriction fragment length polymorphism (RFLP) and sequence analysis of 16S rDNA revealed that the jujube tree was infected with 16S rDNA I and V groups of phytoplasmas. Extensive comparative analyses of restriction enzyme profiles from Alu I, Hha I, Msp I, and Rsa I clearly classified the two into... -
Genetic Diversity of Sweet potato feathery mottle virus from Sweet Potatoes in Korea
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2007, 23권 1호 3 13-21 (9 pages)
a total of 28 samples infected with SPFMV were subjected to restriction fragment length polymorphism (RFLP) analysis using DNAs amplified by RT-PCR with specific primer sets corresponding to the coat protein (CP) region of the virus. The similarity matrix by UPGMA procedure indicated that 28 samples infected with SPFMV were classified into three groups based on the number and size of DNA fragments by digestion of CP-encoding regions with 7 enzymes including SalI, AluI, EcoRI, HindIII, FokI,... -
Identification and Characterization of New Copia-Like Retrotransposon Osrl in Rice
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2003, 19권 1호 11 57-63 (7 pages)
of Osr1. Various restriction fragment length polymorphism (RFLP) of LTR were detected in indica cultivars, whereas, only a few could be detected in the japonica cultivars. The population of Osr1 is lower in the wildtype rice compared with that in the domesticated cultivars. The insertion of LTR sequence in the Pi-b gene in the susceptible cultivar suggested that retrotransposon-mediated insertional mutation might play an important role in the resistance breakdown, as well as in the evolution of... -
Genetic Mapping of Resistant Genes in Brassica pekinensis Against Plasmodiophora brassicae Race 6
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2002, 18권 5호 6 266-270 (5 pages)
brassicae race 6) and CS-113 (susceptible line) were crossed and their progeny lines F1, BC1F1, F2, and F3 were produced for the construction of the genetic linkage map of P. brassicae race 6-resistant Brassica campestris ssp. pekinensis genome. Restriction fragment length polymorphism (RFLP) was applied to compare between parents and their F2 progenies with a total of 192 probes and 5 restriction enzymes. The constructed RFLP map covered 1,104 cM with a mean distance between genetic marker of... -
Molecular Detection and Analysis of Sweet potato feathery mottle virus from Root and Leaf Tissues of Cultivated Sweet Potato Plants
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2002, 18권 1호 3 12-17 (6 pages)
fg. The virus was rapidly detected from total nucleic acids of leaves and roots from the virus-infected sweet potato plants as well as from the purified viral RNA by the RTPCR. Twenty-four sweet potato samples were selected and analyzed by RT-PCR and restriction fragment length polymorphism (RFLP). RFLP analysis of the PCR products showed three restriction patterns, which resulted in some point mutations suggesting the existence of quasi-species for the virus in the infected sweet potato plants. -
Identification of Luteovirus Nucleotide Sequences in Mild Yellow-Edge Diseased Strawberry Plants
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2002, 18권 1호 1 1-5 (5 pages)
reaction (PCR) products of a 487 bp. and approximately 500 bp DNA fragments were amplified from nucleic acid extracts of PLRV-infected tissue and strawberry mild yellow-edge (SMYE) diseased strawberry tissue, respectively. The amplified DNA fragments were further differentiated by hybridization analysis with a cDNA probe for the coat protein gene of PLRV and restriction fragment length polymorphism (RFLP) analysis. These results suggest that a luteovirus is associated with the SMYE disease.
