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131.

Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus

Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 13 467-472 (6 pages)

Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 저
...
- 3,000원
Swine hepatitis E virus (HEV) is widespread throughout pigs in both developing and industrialized countries. This virus is an important zoonotic agent and a public concern worldwide. Infected pigs are asymptomatic, so diagnosing swine HEV relies on detection of the virus or antibodies against the virus. However, several obstacles need to be overcome for effective and practical serological diagnosis. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) that used a purified...
132.

Quantitation of meloxicam in the plasma of koalas (Phascolarctos cinereus) by improved high performance liquid chromatography

Benjamin Kimble, Kong Ming Li, Merran Govendir* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 2 7-14 (8 pages)

Benjamin Kimble, Kong Ming Li, Merran Govendir* 저
...
- 3,000원
An improved method to determine meloxicam (MEL) concentrations in koala plasma using reversed phase high performance liquid chromatography equipped with a photo diode array detector was developed and validated. A plasma sample clean-up step was carried out with hydrophilic- lipophilic copolymer solid phase extraction cartridges. MEL was separated from an endogenous interference using an isocratic mobile phase [acetonitrile and 50 mM potassium phosphate buffer (pH 2.15), 45：55 (v：v)] on a...
133.

Production of a highly group-specific monoclonal antibody against zearalenone and its application in an enzyme-linked immunosorbent assay

Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 2 119-125 (7 pages)

Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 저
...
- 3,000원
A monoclonal antibody (mAb) against zearalenone (ZEN) was produced using ZEN-carboxymethoxylamine and -BSA conjugates. Antibody produced by one clone showing a very high binding ability was selected and found to have a higher affinity for ZEN compared to a commerciall ZEN antibody. We developed two direct competitive ELISA systems using the selected antibody (ZEN-coated and anti-ZEN antibody-coated ELISA). Quantitative ranges for the anti-ZEN antibody-coated ELISA and ZEN-coated ELISA were from...
134.

Transmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa

Letícia Z. Oliveira*, Vera F. M. Hossepian de Lima, Marcelo A. Levenhagen, Ricarda M. dos Santos, Terezinha I. Assumpç 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 10 267-272 (6 pages)

Letícia Z. Oliveira*, Vera F. M. Hossepian de Lima, Marcelo A. Levenhagen, Ricarda M. dos Santos, Terezinha I. Assumpç 저
...
- 3,000원
The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before (n = 18) and after (n = 18) Percoll centrifugation. The evaluation of 20 sperm heads from each of the...
135.

In vitro toxicity of melamine against Tetrahymena pyriformis cells

Zhengfang Wang, Liben Chen, Rashad Al-Kasir, Bo Han* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 5 27-34 (8 pages)

Zhengfang Wang, Liben Chen, Rashad Al-Kasir, Bo Han* 저
...
- 3,000원
This study assessed the toxicity of melamine against the unicellular eukaryotic system of Tetrahymena (T.) pyriformis exposed to 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine. Cell growth curves of different cultures, the half maximum inhibition concentration (IC50) value of melamine, and morphological changes in cells were obtained via optical and transmission electron microscopic observation. The effects of eleven melamine concentrations, 0.25, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5...
136.

Conditional knockout of brca1/2 and p53 in mouse ovarian surface epithelium: Do they play a role in ovarian carcinogenesis?

Ki-Yon Kim, Dong Wook Park, Eui-Bae Jeung, Kyung-Chul Choi* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 3 291-297 (7 pages)

Ki-Yon Kim, Dong Wook Park, Eui-Bae Jeung, Kyung-Chul Choi* 저
...
- 3,000원
Alterations of genes are known to be critical for the induction of tumorigenesis, but the mechanism of ovarian carcinogenesis is little understood and remains to be elucidated. In this study, we investigated the roles of brca1, brca2 and p53 genes in the development of ovarian cancer using conditional knockout mice generated by a Cre-loxP recombinant system. Following the application of recombinant adenovirus expressing Cre in vitro, the proliferation of ovarian surface epithelium (OSE) was...
137.

Antimutagenic and anticarcinogenic effect of methanol extracts of Petasites japonicus Maxim leaves

Hwan-Goo Kang, Sang-Hee Jeong*, †, Joon-Hyoung Cho 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 8 51-58 (8 pages)

Hwan-Goo Kang, Sang-Hee Jeong*, †, Joon-Hyoung Cho 저
...
- 3,000원
The methanol extract from the leaves of Petasites japonicus Maxim (PJ) was studied for its (anti-)mutagenic effect with the SOS chromotest and reverse mutation assay. The (anti-)carcinogenic effects were evaluated by the cytotoxicity on human cancer line cells and by the function and the expression of gap junctions in rat liver epithelial cell. PJ extracts significantly decreased spontaneous β-galactosidase activity and β-galactosidase activity induced by a mutagen, ICR, in Salmonella (S.)...
138.

Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules

William C. Davis*, Mary Jo Hamilton 대한수의학회 Journal of Veterinary Science 16 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 8 51-66 (16 pages)

William C. Davis*, Mary Jo Hamilton 저
...
- 4,500원
Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for reactivity with rabbit LDM yielded 11 mAbs that recognize conserved epitopes on rabbit LDM orthologues and multiple mAbs that recognize epitopes expressed on the major histocompatibility class I or class II molecules. Screening of mAbs submitted to the Animal Homologues Section of...
139.

Suppressive effect of culture supernatant of erythrocytes and serum from dogs infected with Babesia gibsoni on the morphological maturation of canine reticulocytes in vitro

Mohammad Alamgir Hossain, Osamu Yamato, Gonhyung Kim, Masahiro Yamasaki, Yoshimitsu Maede 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 11 169-174 (6 pages)

Mohammad Alamgir Hossain, Osamu Yamato, Gonhyung Kim, Masahiro Yamasaki, Yoshimitsu Maede 저
...
- 3,000원
The present study evaluated the effects of infected culture supernatant of erythrocytes, fractionation of culture supernatant and serum from dogs infected with Babesia gibsoni (B. gibsoni) on the maturation of canine reticulocytes in vitro. The SDS-PAGE demonstrated that significantly broader bands were generated by both the infected culture supernatant of erythrocytes and the serum from dogs chronically infected with B. gibsoni. The culture supernatant of erythrocytes infected with B. gibsoni...
140.

pH-dependent modulation of intracellular free magnesium ions with ion- selective electrodes in papillary muscle of guinea pig

Shang-Jin Kim, In-Gook Cho, Hyung-Sub Kang, Jin-Shang Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 5 31-36 (6 pages)

Shang-Jin Kim, In-Gook Cho, Hyung-Sub Kang, Jin-Shang Kim 저
...
- 3,000원
A change in pH can alter the intracellular concentration of electrolytes such as intracellular Ca2+ and Na+ ([Na+]i) that are important for the cardiac function.For the determination of the role of pH in the cardiac magnesium homeostasis, the intracellular Mg 2+ concentration ([Mg 2+]i), membrane potential and contraction in the papillary muscle of guinea pigs using ion-selective electrodes changing extracellular pH ([pH]o)or intracellular pH ([pH ]i )were measured in this study. A high...

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