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171.

Elucidating the role of ApxI in hemolysis and cellular damage by using a novel apxIA mutant of Actinobacillus pleuropneumoniae serotype 10

Nai-Yun Chang, Zeng-Weng Chen, Ter-Hsin Chen, Jiunn-Wang Liao, Cheng-Chung Lin, Maw-Sheng Chien, Wei-Cheng Lee, Jiunn-Horng Lin, 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 9 81-89 (9 pages)

Nai-Yun Chang, Zeng-Weng Chen, Ter-Hsin Chen, Jiunn-Wang Liao, Cheng-Chung Lin, Maw-Sheng Chien, Wei-Cheng Lee, Jiunn-Horng Lin, 저
...
- 3,000원
Exotoxins produced by Actinobacillus (A.) pleuropneumoniae (Apx) play major roles in the pathogenesis of pleuropneumonia in swine. This study investigated the role of ApxI in hemolysis and cellular damage using a novel apxIA mutant, ApxIA336, which was developed from the parental strain A. pleuropneumoniae serotype 10 that produces only ApxI in vitro. The genotype of ApxIA336 was confirmed by PCR, Southern blotting, and gene sequencing. Exotoxin preparation derived from ApxIA336 was analyzed for...
172.

An overview of calf diarrhea - infectious etiology, diagnosis, and intervention

Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)

Yong-il Cho, Kyoung-Jin Yoon* 저
...
- 4,600원
Calf diarrhea is a commonly reported disease in young animals, and still a major cause of productivity and economic loss to cattle producers worldwide. In the report of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or...
173.

Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus

Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 13 467-472 (6 pages)

Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 저
...
- 3,000원
a purified recombinant capsid protein of swine HEV. The potential clinical use of this assay was evaluated by comparing it with a commercial kit (Genelabs Technologies, Diagnostics, Singapore). Results of the ELISA were highly correlated with those of the commercial kit with a sensitivity of 97% and specificity of 95%. ROC (receiving operator characteristic) analysis of the ELISA data produced a value of 0.987 (95% CI, 0.977∼0.998, p ＜ 0.01). The cut-off value for the ELISA was also determined...
174.

Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells

Jingpeng Gao, Chunchun Meng, Zongyan Chen, Chuanfeng Li, Guangqing Liu 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 10 441-447 (7 pages)

Jingpeng Gao, Chunchun Meng, Zongyan Chen, Chuanfeng Li, Guangqing Liu 저
...
- 3,000원
lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit vaccines against rabbit hemorrhagic disease virus (RHDV) are promising, application of the vaccines has been restricted due to high production costs or low yield. In the present study, we performed codon optimization of the capsid gene to increase the number of preference codons and eliminate rare codons in Spodoptera frugiperda 9...
175.

Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus

Kang-Seuk Choi, Soo-Jeong Kye, Woo-Jin Jeon, Mi-Ja Park, Saeromi Kim, Hee-Jung Seul, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 8 291-297 (7 pages)

Kang-Seuk Choi, Soo-Jeong Kye, Woo-Jin Jeon, Mi-Ja Park, Saeromi Kim, Hee-Jung Seul, Jun-Hun Kwon 저
...
- 3,000원
recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from infected cells was used as an antigen in a hemagglutination inhibition (HI) test for the detection and titration of NDV-specific antibodies present in chicken sera. The rHN antigen produced high HA titers of 2 per 25 μL, which were similar to those of the NDV...
176.

Expression of verocytotoxic Escherichia coli antigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

Luciana Rossi, Alessia Di Giancamillo, Serena Reggi, Cinzia Domeneghini, Antonella Baldi, Vittorio Sala, Vittorio Dell'Orto, Ann 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 5 263-270 (8 pages)

Luciana Rossi, Alessia Di Giancamillo, Serena Reggi, Cinzia Domeneghini, Antonella Baldi, Vittorio Sala, Vittorio Dell'Orto, Ann 저
...
- 3,000원
Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral administration in transgenic seeds could be an efficient system to stimulate local immunity. This study was conducted to transform tobacco plants for the seed-specific expression of antigenic proteins from a porcine verocytotoxic E. coli strain. Parameters related to an immunological response and...
177.

Vitamin D3 up-regulated protein 1 controls the priming phase of liver regeneration

Hyo-Jung Kwon, Sung-Kuk Hong, Won-Kee Yoon, Ki-Hoan Nam, In-Pyo Choi, Dae-Yong Kim, Hyoung-Chin Kim, Young-Suk Won 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 4 257-262 (6 pages)

Hyo-Jung Kwon, Sung-Kuk Hong, Won-Kee Yoon, Ki-Hoan Nam, In-Pyo Choi, Dae-Yong Kim, Hyoung-Chin Kim, Young-Suk Won 저
...
- 3,000원
Vitamin D3 up-regulated protein 1 (VDUP1) is a potent growth suppressor that inhibits tumor cell proliferation and cell cycle progression when overexpressed. In a previous study, we showed that VDUP1 knockout (KO) mice exhibited accelerated liver regeneration because such animals could effectively control the expression of cell cycle regulators that drive the G1-to-S phase progression. In the present study, we further investigated the role played by VDUP1 in initial priming of liver...
178.

Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay

Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 5 143-150 (8 pages)

Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 저
...
- 3,000원
Monoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative DON calculation ranged from 50 to 4,000 ng/mL for DON-Ab coated ELISA and from 25 to 500 ng/mL for DON-Ag coated ELISA. 50% of inhibitory concentration values of DON, HT-2, 15-acetyl-DON, and nivalenol were 23.44, 22,545, 5,518 and 5,976 ng/mL based on the DON-Ab coated ELISA....
179.

Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene

Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 6 37-43 (7 pages)

Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 저
...
- 3,000원
phage SA4 was cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clone revealed a single 802-bp open reading frame encoding a partial protein with a calculated mass of 30 kDa. Results of this analysis also indicated that the LysSA4 sequence shared a high homology with endolysin of the GH15 phage and other reported phages. The LysSA4 gene of the SA4 phage was subsequently expressed in Escherichia coli. Recombinant LysSA4 induced the lysis of host bacteria in a...
180.

Molecular characterization of Japanese encephalitis virus strains prevalent in Chinese swine herds

Hao Zheng, Tongling Shan, Yu Deng, Chunqing Sun, Shishan Yuan, Yang Yin, Guangzhi Tong* 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 5 27-36 (10 pages)

Hao Zheng, Tongling Shan, Yu Deng, Chunqing Sun, Shishan Yuan, Yang Yin, Guangzhi Tong* 저
...
- 3,000원
Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. HEN0701 had high nucleotide sequence and high amino acid...

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