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181.

Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair

Kuldeep Singh*, Myrna M. Miller, Laura J. Kohrt, Gail Scherba, Edgar F. Garrett, Richard L. Fredrickson 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 15 295-297 (3 pages)

Kuldeep Singh*, Myrna M. Miller, Laura J. Kohrt, Gail Scherba, Edgar F. Garrett, Richard L. Fredrickson 저
...
- 1,500원
The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1∼3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30∼100) and whole blood....
182.

Transmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa

Letícia Z. Oliveira*, Vera F. M. Hossepian de Lima, Marcelo A. Levenhagen, Ricarda M. dos Santos, Terezinha I. Assumpç 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 10 267-272 (6 pages)

Letícia Z. Oliveira*, Vera F. M. Hossepian de Lima, Marcelo A. Levenhagen, Ricarda M. dos Santos, Terezinha I. Assumpç 저
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- 3,000원
The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before (n = 18) and after (n = 18) Percoll centrifugation. The evaluation of 20 sperm heads from each of the...
183.

Influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (Bubalus bubalis) preantral follicles

Pawan K. Dubey, Vrajesh Tripathi, Ram Pratap Singh, G. Taru Sharma* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 9 257-265 (9 pages)

Pawan K. Dubey, Vrajesh Tripathi, Ram Pratap Singh, G. Taru Sharma* 저
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- 3,000원
0 (control), 10, 10, 10, and 10 M SNP. To examine the reversible effect of SNP, PFs were cultured with 10 M SNP ＋ 1 mM N-nitro-L-arginine methyl ester (L-NAME) or 1.0 mg hemoglobin (Hb). The results showed that greater concentrations of SNP (10, 10, 10 M) inhibited (p ＜ 0.05) FSH-induced survival, growth, antrum formation, estradiol production, and oocyte apoptosis in a dose-dependent manner. However, a lower dose of SNP (10 M) significantly stimulated (p ＜ 0.05) the survival, growth, antrum...
184.

Evaluation of glycoproteins purified from adult and larval camel ticks (Hyalomma dromedarii) as a candidate vaccine

Amr E. El Hakim, Yasser E. Shahein*, Sobhy Abdel-Shafy, Amira M. K. Abouelella, Ragaa R. Hamed 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 7 243-249 (7 pages)

Amr E. El Hakim, Yasser E. Shahein*, Sobhy Abdel-Shafy, Amira M. K. Abouelella, Ragaa R. Hamed 저
...
- 3,000원
a single-step purification method with Con-A sepharose. The purified GLPs were evaluated as vaccines against camel tick infestation in rabbits. The rabbits received three intramuscular inoculations of GLPs (20 mg/animal) on days 0, 14, and 28. In the immunoblot analysis, Sera from the immunized rabbits recognized the native GLPs and other proteins from larval and adult H. dromedarii ticks along with those from other tick species such as Rhipicephalus sanguineus but not Ornithodoros moubata. The...
185.

A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle

Hidetoshi Higuchi, Hidetomo Iwano, Kazuhiro Kawai, Takehiro Ohta, Tetsu Obayashi, Kazuhiko Hirose, Nobuhiko Ito, Hiroshi Yokota, 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 13 191-194 (4 pages)

Hidetoshi Higuchi, Hidetomo Iwano, Kazuhiro Kawai, Takehiro Ohta, Tetsu Obayashi, Kazuhiko Hirose, Nobuhiko Ito, Hiroshi Yokota, 저
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- 1,500원
mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be about 2.5 × 10 cfu/mL and was similar to that of the standard PCR. We compared the specificity and sensitivity of the simplified PCR to those of a...
186.

Development of a universal plate-agglutination test for detecting Haemophilus parasuis

Dingqian Guo, Cheng Tang, Quan Hai, Guoqing Shao, Hua Yue* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 13 355-357 (3 pages)

Dingqian Guo, Cheng Tang, Quan Hai, Guoqing Shao, Hua Yue* 저
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- 1,500원
universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains...
187.

Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey

G?khan Inat*, Belgin Siriken 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 7 321-326 (6 pages)

G?khan Inat*, Belgin Siriken 저
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- 3,000원
This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected from Samsun Province of Turkey. A total of 200 samples collected from cattle carcasses and the rectal contents of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200...
188.

Peripheral serotoninergic response to physical exercise in athletic horses

Daniela Alberghina*, Claudia Giannetto, Giuseppe Piccione 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 2 285-289 (5 pages)

Daniela Alberghina*, Claudia Giannetto, Giuseppe Piccione 저
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- 1,500원
The purpose of this study was to evaluate the influence of exercise on plasma tryptophan (TRP) and free serotonin (f5-HT), whole blood-5-HT (WB-5-HT) and f5-HT/WB-5-HT ratio in Italian Saddle horses. Six clinically healthy Italian Saddle horses were subjected to a 450 meters obstacles course. Blood samples were collected from each horse by jugular venipuncture using vacutainer tubes with K3-EDTA at rest, immediately after exercise, and after 30 min. TRP, f5-HT and WB-5-HT were analyzed by HPLC....
189.

Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2

Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 8 227-233 (7 pages)

Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 저
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- 3,000원
Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2 and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of...
190.

Detection of Helicobacter spp. in gastric, fecal and saliva samples from swine affected by gastric ulceration

Patrizia Casagrande Proietti*, Annalisa Bietta, Chiara Brachelente, Elvio Lepri, Irit Davidson, Maria Pia Franciosini 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 7 221-225 (5 pages)

Patrizia Casagrande Proietti*, Annalisa Bietta, Chiara Brachelente, Elvio Lepri, Irit Davidson, Maria Pia Franciosini 저
...
- 1,500원
The aim of this study was to evaluate the presence of Helicobacter (H.) spp. in swine affected by gastric ulceration. Stomachs from 400 regularly slaughtered swine were subjected to gross pathological examination to evaluate the presence of gastric ulcers. Sixty-five samples collected from ulcerated pars esophagea and 15 samples from non-ulcerated pyloric portions were submitted to histopathological and molecular analyses, to detect Helicobacter spp., H. suis and H. pylori by PCR. Feces and...

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