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A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China
Jian-guo Dong, Lin-yang Yu, Pei-pei Wang, Le-yi Zhang, Yan-ling Liu, Peng-shuai Liang, Chang-xu Song 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 12 89-98 (10 pages)
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus (PRRSV), GDsg, was isolated in Guangdong province, China, and caused high fever, high morbidity, and high mortality in sows and piglets. The genome of this new strain was 15,413 nucleotides (nt) long, and comparative analysis revealed that GDsg shared 82.4% to 94% identity with type 2 PRRSV strains, but only 61.5% identity with type... -
Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis
Satoshi Gondaira, Hidetoshi Higuchi, Hidetomo Iwano, Koji Nishi, Takanori Nebu, Keiichi Nakajima, Hajime Nagahata 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 11 79-87 (9 pages)
Mycoplasma spp. are contagious bacteria, and mycoplasmal mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMECs) have an important role in the elimination of pathogens, but the effect of Mycoplasma bovis on bMECs has not been fully described. To elucidate the immune response against intramammary infection by M. bovis, we undertook microarray analysis to examine and profile mRNA expression in bMECs after stimulation with M. bovis. We also compared the... -
Isolation and characterization of a new porcine epidemic diarrhea virus variant that occurred in Korea in 2014
Dong-Kun Yang, Ha-Hyun Kim, Seung-Heon Lee, Soon-Seek Yoon, Jung-Won Park, In-Soo Cho 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 10 71-78 (8 pages)
study, we successfully isolated two PEDV isolates (QIAP1401 and QIAP1402) from naturally infected piglets at Jeju-do, Korea. Viral propagation was confirmed in Vero cells based on cytopathic effect, immunofluorescence assay, reverse transcription-polymerase chain reaction, and electron microscopic analyses. The QIAP401 isolate propagated well in Vero cells for 70 passages, with titers of 10 to 10 50% tissue culture infectious dose/mL, which increased gradually with passaging. The nucleotide and... -
Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 9 59-70 (12 pages)
Groups functional assignments, 12 proteins were associated with metabolism, 7 proteins associated with information storage and processing, and 3 proteins associated with cellular processing and signaling. By semi-quantitative reverse transcriptase polymerase chain reaction, increases in tldD, efp, ntrX, pdhA, purB, and ATPA mRNA expression and decreases in Rho and yfeA mRNA expression were confirmed in Δomp43. In conclusion, this is the first report showing that a loss of OMP43 expression in B.... -
Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene
Sharad Saurabh, Shubhankar Sircar, Jobin J. Kattoor, Souvik Ghosh, Nobumichi Kobayashi, Krisztian Banyai, Obli R. VinodhKumar, U 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 6 35-43 (9 pages)
Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes... -
Coactosin-like protein 1 inhibits neuronal migration during mouse corticogenesis
Guohong Li, Yupeng Yin, Jiong Chen, Yanle Fan, Juhong Ma, Yingxue Huang, Chen Chen, Pengxiu Dai, Shulin Chen, Shanting Zhao 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 4 21-26 (6 pages)
Coactosin-like protein 1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from a soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains undescribed. In this study, we revealed that Cotl1 overexpression impaired migrationof both early- and late-born neurons during mouse corticogenesis.... -
Infections by pathogens with different transmission modes in feral cats from urban and rural areas of Korea
Jusun Hwang, Nicole Gottdenker, Dae-Hyun Oh, Hang Lee, Myung-Sun Chun 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 14 541-545 (5 pages)
In this study, we examine prevalences of three infectious pathogens with different transmission modes (Bartonella henselae, hemoplasma, and Toxoplasma gondii) in feral cats from urban and rural habitats. Infection status of the three pathogens in blood samples (n = 117) was determined through molecular or serological diagnostic methods. Overall prevalence of hemoplasma, Toxoplasma gondii, and Bartonella henselae was 47.9%, 50%, and 35.7%, respectively. Comparing the two habitats, only... -
Inhibitory effects of resveratrol on hepatitis B virus X protein-induced hepatocellular carcinoma
Seungmo Park, Jihae Lim, Jong Rhan Kim, Seongbeom Cho 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 1 419-429 (11 pages)
Liver cancer occurs very frequently worldwide and hepatocellular carcinoma (HCC) accounts for more than 80% of total primary liver cancer cases. In this study, the anticarcinogenic effects of resveratrol against hepatitis B virus (HBV)-induced HCC were investigated by using HBV X-protein-overexpressing Huh7 (Huh7-HBx) human hepatoma cells. MTT assay showed that resveratrol decreased cell viability. Fluorescence-activated cell-sorter analysis showed that resveratrol induced G1 cell cycle arrest... -
Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue
Masahiro Yasuda, Jyunya Kawabata, Sayaka Akieda-Asai, Tetsuo Nasu, Yukari Date 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 11 341-348 (8 pages)
Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several mammalian species. The GNY/GC-C system regulates ion transportation and pH in the mucosa. Recently, it was reported that GC-C and GNY are involved in lipid metabolism in rat mesenteric adipose tissue macrophages. To examine the role of GC-C... -
Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 7 307-316 (10 pages)
A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test...


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