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331.

Altered maturation of dendritic cells by taxol, an anticancer drug

Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 5 229-234 (6 pages)

Hong-Gu Joo 저
...
- 3,000원
In this study, taxol enhanced the expression of MHC class II on DCs, compared to medium-treated immature DCs. Surprisingly, the viability of DCs was not decreased by taxol, whereas that of cancer cells was. It was confirmed that taxol did not induce the apoptosis of DCs based on annexin V-FITC/propidium iodide (PI) staining assay. Since previous study demonstrated that taxol induced the production of nitric oxide (NO) related to the viability of DCs, the level of NO from taxol-treated DCs was...
332.

Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae

Sung Jae Shin, Jong Lye Bae, Young Wook Cho, Moon Sik Yang, Dae Hyuk Kim, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 4 225-228 (4 pages)

Sung Jae Shin, Jong Lye Bae, Young Wook Cho, Moon Sik Yang, Dae Hyuk Kim, Yong Suk Jang, Han Sang Yoo 저
...
- 1,500원
Actinobacillus pleuropneumoniae is an important primary pathogen in pigs, in which it causes a highly contagious pleuropneumoniae. In our previous study, apxIA gene amplified from A. pleuropneumoniae Korean isolate by PCR with primer designed based on the N- and C-terminal of the toxin was cloned in TA cloning vector and sequenced. The nucleotide sequences of apxIA gene was reported to GeneBank with the accession numbers of AF363361. Identity of the Apx IA from the cloned gene in E. coli was...
333.

Effects of Combination Dietary Conjugated Linoleic Acid with Vitamin A (Retinol) and Selenium on the Response of the Immunoglobulin Productivity in Mice

Jin-young Kim, Byung-hyun Chung 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 16 103-108 (6 pages)

Jin-young Kim, Byung-hyun Chung 저
...
- 3,000원
1.5% CLA group. The level of serum IgG in 1.5% CLA combined with selenium was maintained at the same level as that of control. It is considered that over- doses of CLA (1.5%) even depressed the production of immunoglobulin but selenium and/or vitamin inhibited this activity to a certain extent.In this study, dietary CLA increased immunoglobulin production in a dose-dependent manner. Vitamin ADE and Selenium combined with CLA also increased the immunoglobulin production response except serum IgE.
334.

Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System

Kwang-taek Lim, Byeong-chun Lee, Sung-keun Kang, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 11 73-78 (6 pages)

Kwang-taek Lim, Byeong-chun Lee, Sung-keun Kang, Woo-suk Hwang 저
...
- 3,000원
system, which applies different conditions for early cleavage embryos, i.e., serum-free media, vs. later stage embryos, with serum containing media, may be effective for in vitro production systems. In addition, the developmental competence of bovine embryos was depressed in the presence of glucose plus phosphate as compared to either alone or the absence of both. Therefore, the avoidance of this negative effect should allow more optimal conditions to be developed for in vitro production.
335.

Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes

Kazim R. Chohan, Alan G. Hunter 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 10 67-72 (6 pages)

Kazim R. Chohan, Alan G. Hunter 저
...
- 3,000원
The objectives of the present study were to compare the in vitro maturation (IVM), fertilization and early embryonic development of bovine oocytes recovered from ovaries during the follicular, metestrus and diestrus stages of the estrous cycle and at anestrus and pregnancy after maturation in a serum free culture medium. Cumulus oocyte complexes (COCs) collected from ovaries at different reproductive statuses were matured in medium 199 supplemented with 10 g/ml FSH, 10 g/ml LH, 1.5 g/m...
336.

Characterization of Antigenic Sites on the Rinderpest Virus N Protein Using Monoclonal Antibodies

Kang-seuk Choi, Jin-ju Nah, Young-joon Ko, Cheong-up Choi, Jae-hong Kim, Shien-young Kang, Yi-seok Joo 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 9 57-65 (9 pages)

Kang-seuk Choi, Jin-ju Nah, Young-joon Ko, Cheong-up Choi, Jae-hong Kim, Shien-young Kang, Yi-seok Joo 저
...
- 3,000원
The N protein of the rinderpest virus (RPV) was analyzed topologically and antigenically by using anti-N monoclonal antibodies (Mabs). Ten Mabs were raised against the N protein of the RPV. At least six non-overlapping antigenic sites (sites A-F) were delineated by competitive binding assays using biotinylated Mabs. Of them 5 sites (A, C, D, E and F) on the N protein were recognized by RPV-specific Mabs in ELISA and IFA while site B was recognized by Mabs reacting with both RPV and PPRV. Non-...
337.

Mac-1-mediated Uptake and Killing of Bordetella bronchiseptica by Porcine Alveolar Macrophages

Jong-keuk Lee, Lawrence B. Schook, Mark S. Rutherford 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 7 41-49 (9 pages)

Jong-keuk Lee, Lawrence B. Schook, Mark S. Rutherford 저
...
- 3,000원
The role of Mac-1 as a receptor for Bordetella bronchiseptica infection of alveolar macrophages (AMphi) was examined using 6 strains (2 ATCC and 4 pathogenic field isolates) to assess B. bronchiseptica binding, uptake and replication in primary porcine AMphi. All B. bronchiseptica strains were rapidly killed by porcine serum in a dose- and time-dependent manner. However, heat-inactivated porcine serum (HIS) did not demonstrate any bacterial-killing activity, suggesting that complement may have a...
338.

Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)

In-Soo Choi and Han-Sang Yoo 저
...
- 3,000원
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by...
339.

Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages

In-Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Choand Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 6 97-102 (6 pages)

In-Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Choand Han-Sang Yoo 저
...
- 3,000원
The kinetics of cytokine mRNA expression was studied in porcine alveolar macrophages using an RT-PCR assay. The expression levels of IFN- gamma, IL-2, IL-4, IL-6, GM-CSF, IL-12 p35, and IL-12 p40 were examined after 2, 4, 14, 24, 48, and 72 h of incubation in unstimulated control and LPS-stimulated cells. The expression contents of IFN-gamma, IL-2, and IL-4 were not detected in both unstimulated and LPS-stimulated cells. On the other hand, the expression levels of IL-6, GM-CSF, and IL-12 in...
340.

Evaluation of expression patterns of feline CD28 and CTLA-4 in feline immunodeficiency virus (FIV)-infected and FIV antigen-induced PBMC

In-Soo Choi, Han Sang Yoo, Ellen W. Collisson 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 6 97-103 (7 pages)

In-Soo Choi, Han Sang Yoo, Ellen W. Collisson 저
...
- 3,000원
It is known that CD28, a positive costimulatory receptor, plays a very important role in inducing the optimal stimulation of T lymphocytes. CTLA-4 (CD152), however, acts as a negative regulator in T lymphocyte activation. The effect of an feline immunodeficiency virus (FIV) infection on the expression of feline CD28 and CTLA-4 was studied with FIV-infected and uninfected peripheral blood mononuclear cells (PBMC) using a competitive PCR assay. The nature of CD28 and CTLA-4 expression was also...

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