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Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy
Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 14 315-322 (8 pages)
Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or to store them in a repository. A mutant line can be taken out of a breeding nucleus only if sufficient numbers of samples with an appropriate level of quality are cryopreserved. The quality of different donors within the same mouse line might be heterogeneous and the... -
SRY-positive 78, XY ovotesticular disorder of sex development in a wolf cloned by nuclear transfer
Jung Taek Kang, Hyung Jin Kim, Hyun Ju Oh, So Gun Hong, Jung Eun Park, Min Jung Kim, Min Kyu Kim, Goo Jang, Dae Yong Kim, Byeong 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 15 211-213 (3 pages)
Recently, we reported the three wolves cloning with normal karyotype from somatic cells of endangered male gray wolves (Canis lupus), but one wolf had female external genitalia. In this study, we conducted further clinical, histological, and genetic analyses. This cloned wolf had a normal uterus but developed ovotestis. Through molecular analysis of the SRY gene, a mutation in the coding sequence of SRY gene could be excluded as a cause of intersexuality. This is the first report of a cloned... -
Post-mortem re-cloning of a transgenic red fluorescent protein dog
So Gun Hong, Ok Jae Koo, Hyun Ju Oh, Jung Eun Park, Minjung Kim, Geon-A Kim, Eun Jung Park, Goo Jang, Byeong-Chun Lee* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 15 405-407 (3 pages)
Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although... -
Genetic variability of six indigenous goat breeds using major histocompatibility complex-associated microsatellite markers
Patricy de Andrade Salles, Silvana C. Santos, Davide Rondina, Mathias Weller 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 4 127-132 (6 pages)
The present study aimed at analyzing the genetic variability of indigenous goat breeds (Capra hircus) using the MHC-associated microsatellite markers BF1, BM1818, BM1258, DYMS1, and SMHCC1. The following breeds were included: Chinese Xuhuai, Indian Changthangi and Pashmina, Kenyan Small East African (SEA) and Galla, and Albanian Vendi. To examine genetic variability, the levels of heterozigosity, degrees of inbreeding, and genetic differences among the breeds were analyzed. The mean number of... -
In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes
Momena Khatun, Mohammad Musharraf Uddin Bhuiyan, Jalal Uddin Ahmed, Aminul Haque, Mohammad Bozlur Rahman*, Mohammed Shamsuddin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 11 75-82 (8 pages)
Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle's salt with L-glutamine and sodium bicarbonate) for 27 h at 39C under 5% CO2 in humidified air. The... -
Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes
Eunsong Lee, Mohammad Musharraf Uddin Bhuiyan, Hiroyuki Watanabe, Kohji Matsuoka, Yoshihiro Fujise, Hajime Ishikawa, Yutaka Fuku 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 2 285-292 (8 pages)
In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their developmental capacity by investigating the effect of osmolarity and macromolecules in the culture medium on the in vitro developmental capacity. Hybrid embryos produced by the electrofusion of fetal whale fibroblasts with enucleated porcine oocytes were cultured in modified porcine zygote medium-3... -
Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)
Feng-Shan Gao, Gui-Xue Hu*, Xian-zhu Xia, Yu-Wei Gao, Ya-Duo Bai, Xiao-Huan Zou 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 13 261-263 (3 pages)
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to... -
A comparative study of gastrointestinal parasites between ranched and free ranging Burchell's zebra (Equus burchelli antiquorum) in Isiolo district, Kenya
Elizabeth N. Wambwa, W.O. Ogara, Dennis Mudakha 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 5 215-220 (6 pages)
The abdominal cavity was scrutinized for Setaria species. The parasites were identified to genus and in some cases species level. All animals were infected with at least three genera of parasites of which at least one genus was a nematode. A total of 10 genera representing eight families were recovered from the two groups. These included six nematode families, Strongylidae, Atractidae, Oxyuridae, Spiruridae, Setariidae, and Ascaridae, one cestode family, Anoplocephalidae and one family of the... -
Laminitis in Przewalski horses kept in a semireserve
Klaus-Dieter Budras, Klaus Scheibe, Bianca Patan, Wolf J. Streich, Kabsu Kim 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 1 1-7 (7 pages)
separately fenced area. All three affected mares had fully recovered from their laminitic condition. Prevention of grass laminitis can be achieved by the following measures: 1. Reduction in grass intake could be achieved by increasing the grazing pressure by an increase in stocking rate of the horses or mixed grazing with another species such as sheep. 2. A longer term solution to the problem may well be to sow specific varieties of grass with lower concentrations of water soluble carbohydrate. -
Diagnostic laparoscopy in a leopard cat (Prionailurus bengalensis) with intercostal abdominal hernia and hepatic lipidosis
Seong-hoon Seok , Se-jin Park , Seung-yong Lee , Hee-chun Lee , Seong-chan Yeon 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 3 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 2호 8 127-129 (3 pages)


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