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31.

Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 14 315-322 (8 pages)

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 저
...
- 3,000원
microscopy. For this, a live/dead cell staining protocol requiring only small amounts of material was created. Membrane integrity was then examined as major parameter closely correlated with successful IVF. These complex analyses allow us to monitor frozen/thawed spermatozoa from GM mice using a relatively simple staining procedure. This approach leads to a reduction of animal experiments and contributes to the 3R principles (replacement, reduction and refinement of animal experiments).
32.

Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

Yashpal S. Malik*, Kuldeep Sharma, Nirupama Vaid, Somendu Chakravarti, K. M. Chandrashekar, Sanjay S. Basera, Rashmi Singh, Mina 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 8 271-278 (8 pages)

Yashpal S. Malik*, Kuldeep Sharma, Nirupama Vaid, Somendu Chakravarti, K. M. Chandrashekar, Sanjay S. Basera, Rashmi Singh, Mina 저
...
- 3,000원
The present study describes the genotypic distribution of rotaviruses (RVs) in an Indian bovine population with unexpectedly higher proportions of G3 alone or in combination of G8/G10. PCR-genotyping confirmed that 39.4% (13/33) of the prevalent RVs were the G3 type while 60.6% (20/33) were dual G3G10 or G3G8 types. P typing revealed that 93.9% (31/33) of the samples were P[11] while 6.1% (2/33) possessed a dual P[1]P[11] type. Sequence analysis of the VP7 gene from G3 strains viz. B-46, 0970,...
33.

Elm tree bark extract inhibits HepG2 hepatic cancer cell growth via pro-apoptotic activity

Tae Myoung Kim, Sang-Kyung Shin, Tae-Wang Kim, So-Young Youm, Dae Joong Kim, Byeongwoo Ahn* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 2 7-13 (7 pages)

Tae Myoung Kim, Sang-Kyung Shin, Tae-Wang Kim, So-Young Youm, Dae Joong Kim, Byeongwoo Ahn* 저
...
- 3,000원
Control of inflammation is widely accepted as an important strategy for cancer chemoprevention. Anti-inflammatory effects of bark extracts of elm tree (BEE) have been amply reported. Therefore, BEE may be a good candidate cancer chemopreventive agent. Considering the high incidence of hepatic cancer and limited therapeutic approaches for treating this disease, it is important to develop liver cancer-specific chemopreventive agents. To evaluate the chemopreventive potential of BEE, we...
34.

Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury

Hak-Hyun Ryu, Ji-Hey Lim, Ye-Eun Byeon, Jeong-Ran Park, Min-Soo Seo, Young-Won Lee, Wan Hee Kim, Kyung-Sun Kang*, Oh-Kyeong Kweo 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 1 273-284 (12 pages)

Hak-Hyun Ryu, Ji-Hey Lim, Ye-Eun Byeon, Jeong-Ran Park, Min-Soo Seo, Young-Won Lee, Wan Hee Kim, Kyung-Sun Kang*, Oh-Kyeong Kweo 저
...
- 4,100원
In this study, we evaluated if the implantation of allogenic adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by epidural balloon compression: C group (no ASCs treatment as control), V group (vehicle treatment with PBS), and ASC group (ASCs treatment). ASCs or vehicle were injected directly into the injured site 1 week after spinal cord injury....
35.

A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen

Hae-Lee Lee, Sue-Hee Kim, Dong-Beom Ji, Yong-Jun Kim* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 11 249-255 (7 pages)

Hae-Lee Lee, Sue-Hee Kim, Dong-Beom Ji, Yong-Jun Kim* 저
...
- 3,000원
The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen. Sephadex, glass wool and Percoll gradient separation techniques were used for sperm separation and sperm motility, morphology and membrane integrity were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique. The...
36.

Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms

Hye Kwon Kim, Jeong Sun Yang, Hyoung Joon Moon, Seong Jun Park, Yuzi Luo, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Soo Kyung A 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 5 121-130 (10 pages)

Hye Kwon Kim, Jeong Sun Yang, Hyoung Joon Moon, Seong Jun Park, Yuzi Luo, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Soo Kyung A 저
...
- 3,000원
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic...
37.

In vitro and in vivo gene therapy with CMV vector-mediated presumed dog β-nerve growth factor in pyridoxine-induced neuropathy dogs

Jin-Young Chung, Jung-Hoon Choi, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Sang-Koo Lee, Hwa-Young Youn* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 5 367-374 (8 pages)

Jin-Young Chung, Jung-Hoon Choi, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Sang-Koo Lee, Hwa-Young Youn* 저
...
- 3,000원
Due to the therapeutic potential of gene therapy for neuronal injury, many studies of neurotrophic factors, vectors, and animal models have been performed. The presumed dog β-nerve growth factor (pdβ-NGF) was generated and cloned and its expression was confirmed in CHO cells. The recombinant pdβ-NGF protein reacted with a human β-NGF antibody and showed bioactivity in PC12 cells. The pdβb-NGF was shown to have similar bioactivity to the dog β-NGF. The recombinant pdβ-NGF plasmid was...
38.

All blood, No stool: enterohemorrhagic Escherichia coli O157:H7 infection

Jang W. Yoon, Carolyn J. Hovde 대한수의학회 Journal of Veterinary Science 14 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 1 219-232 (14 pages)

Jang W. Yoon, Carolyn J. Hovde 저
...
- 4,300원
Enterohemorrhagic Escherichia coli serotype O157:H7 is a pathotype of diarrheagenic E. coli that produces one or more Shiga toxins, forms a characteristic histopathology described as attaching and effacing lesions, and possesses the large virulence plasmid pO157. The bacterium is recognized worldwide, especially in developed countries, as an emerging food-borne bacterial pathogen, which causes disease in humans and in some animals. Healthy cattle are the principal and natural reservoir of E....
39.

Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp

Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 5 131-137 (7 pages)

Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 저
...
- 3,000원
A normal prion protein (PrPc) is converted to a proteaseresistant isoform by an apparent self-propagating activity in transmissible spongiform encephalopathy, a neurodegenerative disease. The cDNA encoding open reading frame (ORF) of the bovine prion protein gene (Prnp) was cloned from Korean cattle by PCR, and was transfected into Chinese hamster ovary (CHO-K1) cells using lipofectamine. The gene expression of the cloned cDNA was confirmed by RT-PCR and Western blotting with the monoclonal...
40.

Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 3 15-20 (6 pages)

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 저
...
- 3,000원
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and...

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