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Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
involved with binding and structural molecule activities. Vimentin and ㅁㅁα-enolase were central “functional hubs” in the network. Based on results from the present study, disease-induced alterations of protein expression in mammary glands and potential markers for the effective treatment of E. coli mastitis were identified. These data have also helped elucidate defense mechanisms that protect the mammary glands and promote the pathogenesis of E. coli mastitis. -
Feasibility of utilizing the patellar ligament angle for assessing cranial cruciate ligament rupture in dogs
Jung-ha Lee, Soon-wuk Jeong 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 8 563-568 (6 pages)
cranial cruciate ligament (CrCL). The PLA of stifles with complete CrCL rupture was significantly lower than that of normal stifles, particularly at a flexion angle of 60∼80o in both plain and stress views. If the PLA was ＜90.55o on the stress view with a 60∼80o flexion angle, the dog was diagnosed with a complete rupture of the CrCL with a sensitivity of 83.9% and specificity of 100%. In conclusion, measuring the PLA is a quantitative method for diagnosing complete CrCL rupture in canines. -
Effects of resveratrol on the insulin signaling pathway of obese mice
Hyun Ju Hong, Wonyoung Kang, Dong Geon Kim, Dae Ho Lee, Youngjae Lee, Chang-Hoon Han* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 2 179-185 (7 pages)
The present study was conducted to investigate the effects of resveratrol on the insulin signaling pathway in the liver of obese mice. To accomplish this, we administered resveratrol to high fat diet-induced obese mice and examined the levels of protein phosphorylation in the liver using an antibody array. The phosphorylation levels of 10 proteins were decreased in the high fat diet and resveratrol (HFR) fed group relative to the levels in the high fat diet (HF) fed group. In contrast, the... -
Photodynamic hyperthermal chemotherapy with indocyanine green: a novel cancer therapy for 16 cases of malignant soft tissue sarcoma
Masaki Onoyama, Takeshi Tsuka, Tomohiro Imagawa, Tomohiro Osaki, Saburo Minami, Kazuo Azuma, Kazuhiko Kawashima, Hiroshi Ishi, T 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 13 117-123 (7 pages)
Sixteen cases of malignant soft tissue sarcoma (STS; 10 canines and six felines) were treated with a novel triple therapy that combined photodynamic therapy, hyperthermia using indocyanine green with a broadband light source, and local chemotherapy after surgical tumor resection. This triple therapy was called photodynamic hyperthermal chemotherapy (PHCT). In all cases, the surgical margin was insufficient. In one feline case, PHCT was performed without surgical resection. PHCT was performed... -
Quantitation of meloxicam in the plasma of koalas (Phascolarctos cinereus) by improved high performance liquid chromatography
Benjamin Kimble, Kong Ming Li, Merran Govendir* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 2 7-14 (8 pages)
the calibration curve, which was linear from 10 to 1,000 ng/mL (r ＞ 0.9998). Average absolute recovery rates were 91% and 96% for MEL and the IS, respectively. This method had sufficient sensitivity (lower quantitation limit of 10 ng/mL), precision, accuracy, and selectivity for routine analysis of MEL in koala plasma using 250-mL sample volumes. Our technique clearly resolved the MEL peak from the complex koala plasma matrix and accurately measured MEL concentrations in small plasma volumes. -
The effect of conglutinin on production of reactive oxygen species in bovine granulocytes
Marta Dec*, Andrzej Wernicki, Andrzej Puchalski, Renata Urban-Chmiel, Adam Wa?ko 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 5 33-38 (6 pages)
in bovine peripheral phagocytes. Using nitroblue tetrazolium and hydrogen peroxide assays, we showed that sugar ligand- bound conglutinin stimulated the production of superoxide and H2O2 in granulocytes whereas the non-sugar-bound form of conglutinin inhibited these processes. These results indicate that both forms of conglutinin are able to interact with surface leukocyte receptors but have opposite effects on phagocytic activity. Our findings suggest that conglutinin bound to sugar residues on... -
Biomarkers for identifying the early phases of osteoarthritis secondary to medial patellar luxation in dogs
Md. Rafiqul Alam*, Joong Ryong Ji, Min Su Kim, Nam Soo Kim 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 11 273-280 (8 pages)
and serum was significantly decreased (p ＜ 0.05), whereas MMP-2 in SF was significantly increased (p ＜ 0.05) during the progression of OA. Histochemistry revealed an increased number of TRAP-positive cells in tissues from OA-affected joints. Assays measuring TRAP, MMP-2, and TIMP-2 in SF and serum, and methods that detect increased numbers of TRAP-positive cells in the joint tissues can play an important role in identifying the early phases of degenerative changes in canine joint components. -
Establishment of a standard operating procedure for predicting the time of calving in cattle
Dominik Streyl, Carola Sauter-Louis, Anna Braunert, Dorothee Lange, Frank Weber, Holm Zerbe 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 11 177-186 (10 pages)
dystocia in cows and calves. We conducted two studies in healthy cows that compared seven clinical signs (broad pelvic ligaments relaxation, vaginal secretion, udder hyperplasia, udder edema, teat filling, tail relaxation, and vulva edema) alone and in combination in order to predict the time of parturition. The relaxation of the broad pelvic ligaments combined with teat filling gave the best values for predicting either calving or no calving within 12 h. For the proposed parturition score (PS),... -
Evaluation of the estrogenic effects of dietary perinatal Trifolium pratense
Emrah Yatkin, Suzan Daglioglu 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 3 121-126 (6 pages)
was performed to detect estrogen receptor alpha (ERα) and progesterone receptor (PR) levels. Our results revealed estrogen-like biological effects of perinatal T. pratense exposure. Relative uterus and ovary weights in the experimental groups were increased compared to control. The number of uterine glands and luminal epithelium heights were also increased. However, there were no statistically significant changes detected in the immunostaining intensity of ERα and PR between the groups. -
Functional activities of the Tsh protein from avian pathogenic Escherichia coli (APEC) strains
Renata K. T. Kobayashi, Luis Carlos J. Gaziri, Marilda C. Vidotto* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 6 315-319 (5 pages)
The temperature-sensitive hemagglutinin (Tsh) expressed by strains of avian pathogenic Escherichia (E.) coli (APEC) has both agglutinin and protease activities. Tsh is synthesized as a 140 kDa precursor protein, whose processing results in a 106 kDa passenger domain (Tshs) and a 33 kDa b-domain (Tshb). In this study, both recombinant Tsh (rTsh) and supernatants from APEC, which contain Tshs (106 kDa), caused proteolysis of chicken tracheal mucin. Both rTsh (140 kDa) and pellets from wild-type...
