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Korean red ginseng excitation of paraventricular nucleus neurons via non-N-methyl-D-aspartate glutamate receptor activation in mice
Yiming Shen, Janardhan P. Bhattarai, Soo Joung Park, Gyu Seung Lee, Pan Dong Ryu, Seong Kyu Han 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 3 172-178 (7 pages)
It has been reported that Korean red ginseng (KRG), a valuable and important traditional medicine, has varied effects on the central nervous system, suggesting its activities are complicated. The paraventricular nucleus (PVN) neurons of the hypothalamus has a critical role in stress responses and hormone secretions. Although the action mechanisms of KRG on various cells and systems have been reported, the direct membrane effects of KRG on PVN neurons have not been fully described. In this study,... -
Piglet colibacillosis diagnosis based on multiplex polymerase chain reaction and immunohistochemistry of paraffin-embedded tissues
C?ntia De Lorenzo, Caroline P. de Andrade, Ver?nica S. L. Machado, Matheus V. Bianchi, Veronica M. Rolim, Raquel A. S. Cruz, Dav 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 5 27-33 (7 pages)
at least one fimbria and one toxin were detected in 40% of the samples in multiplex PCR. The most frequent virulence types were F18-STaP (7.5%), F18-STaP-STb (5.7%), and F4-STaP (3.8%). A statistically significant association was noted between virulence factors F4, F18, STaP, and STb and positive immunostaining results. Colibacillosis diagnosis through multiplex PCR and IHC of paraffin-embedded tissues is a practical approach, as samples can be fixed and stored for long periods before analysis. -
A serine/threonine phosphatase 1 of Streptococcus suis type 2 is an important virulence factor
Lihua Fang, Jingjing Zhou, Pengcheng Fan, Yunkai Yang, Hongxia Shen, Weihuan Fang 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 3 439-447 (9 pages)
Streptococcus suis is regarded as one of the major pathogens of pigs, and Streptococcus suis type 2 (SS2) is considered a zoonotic bacterium based on its ability to cause meningitis and streptococcal toxic shock-like syndrome in humans. Many bacterial species contain genes encoding serine/threonine protein phosphatases (STPs) responsible for dephosphorylation of their substrates in a single reaction step. This study investigated the role of stp1 in the pathogenesis of SS2. An isogenic stp1... -
Pharmacokinetics of tilmicosin in healthy pigs and in pigs experimentally infected with Haemophilus parasuis
Ling Zhang, Li Zhao, Yonghong Liu, Junfeng Liu, Xianqiang Li 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 2 431-437 (7 pages)
A comparative in vivo pharmacokinetic (PK) study of tilmicosin (TIL) was conducted in 6 crossbred healthy pigs and 6 crossbred pigs infected with Haemophilus (H.) parasuis following oral administration of a single 40 mg/kg dose. The infected model was established by intranasal inoculation and confirmed by clinical signs, blood biochemistry, and microscopic examinations. Plasma TIL concentrations were determined by a validated high-performance liquid chromatography method with ultraviolet... -
Inhibitory effects of resveratrol on hepatitis B virus X protein-induced hepatocellular carcinoma
Seungmo Park, Jihae Lim, Jong Rhan Kim, Seongbeom Cho 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 1 419-429 (11 pages)
by using HBV X-protein-overexpressing Huh7 (Huh7-HBx) human hepatoma cells. MTT assay showed that resveratrol decreased cell viability. Fluorescence-activated cell-sorter analysis showed that resveratrol induced G1 cell cycle arrest without increasing the sub-G1 phase cell population. Therefore, we evaluated its effect on regulation of cyclin D1, which is critically involved in G1/S transition. Resveratrol lowered cyclin D1 transcription. Western blot analysis of the effects of resveratrol on... -
Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing
Hyeon-Joong Kim, Joon Young Kim, Byung-Hwan Lee, Sun-Hye Choi, Hyewon Rhim, Hyoung-Chun Kim, Seoung-Yob Ahn, Soon-Wuk Jeong, Min 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 16 387-397 (11 pages)
a rabbit model of corneal damage, by undertaking histochemical analysis. Treatment of gintonin to HCE cells induced transient increases of [Ca]i in concentration-dependent and reversible manners. Gintonin-mediated mobilization of [Ca]i was attenuated by LPA1/3 receptor antagonist Ki16425, phospholipase C inhibitor U73122, inositol 1,4,5-triphosphate receptor antagonist 2-APB, and intracellular Ca chelator BAPTA-AM. Gintonin facilitated in vitro wound healing in a concentration-dependent manner.... -
Effect of phosphorus deficiency on erythrocytic morphology and function in cows
Ziwei Zhang, Mingyu Bi, Jie Yang, Haidong Yao, Zhonghua Liu, Shiwen Xu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 10 333-340 (8 pages)
The aim of this study was to evaluate the influence of phosphorus (P) deficiency on the morphological and functional characteristics of erythrocytes in cows. Forty Holstein-Friesian dairy cows in mid-lactation were randomly divided into two groups of 20 each and were fed either a low-P diet (0.03% P/kg dry matter [DM]) or a control diet (0.36% P/kg DM). Red blood cell (RBC) indices results showed RBC and mean corpuscular hemoglobin decreased while mean corpuscular volume increased significantly... -
Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 7 307-316 (10 pages)
A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test... -
Guanosine 5'-monophosphate-chelated calcium and iron feed additives maintains egg production and prevents Salmonella Gallinarum in experimentally infected layers
Hye-Ji Noh, HeeKyong Kim, Su Jeong Heo, Hyang Hyun Cho, Hong Bum Koh 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 5 291-297 (7 pages)
We evaluated the effects of guanosine 5′-monophosphate (GMP)-chelated calcium and iron (CaFe-GMP) on health and egg quality in layers experimentally infected with Salmonella Gallinarum. In this study, a CaFe-GMP feed additive was added to a commercial layer feed and fed to layers over a four-week period. All were inoculated with Salmonella Gallinarum. Body weight, mortality, clinical symptoms, and poultry production including feed intake, egg production, egg loss, and feed conversion rate were... -
Inoculation of Lewis lung carcinoma cells enhances formalin-induced pain behavior and spinal Fos expression in mice
Jae-Gyun Choi, Jae-Min Kim, Dong-Wook Kang, Jung-Wan Choi, Jin Bong Park, Seong-Hun Ahn, Yeonhee Ryu, Hyun-Woo Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 2 267-272 (6 pages)
The incidence of lung cancer has rapidly increased and cancer patients at a later cancer stage frequently suffer from unbearable cancer-associated pain. However, the pathophysiology of lung cancer pain has not been fully described due to a lack of appropriate animal models. This study was designed to determine the effect of Lewis lung carcinoma (LLC) cell inoculation on formalin-induced pain behavior and spinal Fos expression in C57BL/6 mice. LLC cells (1.5 × 10, 2.5 × 10, 3.0 × 10 or 5.0 ×...


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