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Properties of Single K+ Channels of Skeletal Muscle Incorporated into Planar Lipid Bilayer
Park, Jin-Bong, Kim, Hee-Jeong, Cho, Myung-Haing, Lee, Hang, Park, Hong-Ki, Lee, Mun-Han, Ryu, Pan-Dong 대한생리학회 대한생리학회지 16 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 2 13-28 (16 pages)
the above results, we think the second type of K+ channel is the sarcoplasmic reticulum K+ (SRK) channel. In addition, both types of channel were also incorporated into the lipids extracted from the skeletal muscle. The channel properties recorded in the bilayers termed from synthetic and extracted lipids were qualitatively similar. Our data indicate that BK and SRK channels are rich in the skeletal muscle and their properties and regulation could be effectively studied in planar lipid bilayer. -
Transformation of Rabbit Proximal Tubule Cells by Strontium Phosphate Transfection with a Plasmid Containing SV4O Early Region Genes
Han, Ho-Jae, Taub, Mary L., 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 14 233-240 (8 pages)
in a hormone defined medium. Domes were also observed in some of the cultures. 2. r-glutamyl transpeptidase activity was equivalent to that observed in primary renal proximal tubule cell cultures. 3. Transformed cells with pRSVT form tubules in matrigel following 20 passages. 4. Genomic DNA of transformants was digested with either the restriction enzyme Xba or BamH1. A band of approximately 7.5kb was detected with Xba. Three BamH1 bands were detected at approximately 15 kb, 6.5 kb, and 3 kb. -
The Excitatory Mechanism of Substance P in the Antral Circular Muscle of Guinea Pig Stomach
Jun, Jae-Yeoul, Kim, Sung-Joon, Choi, Youn-Baik, Kim, Ki-Whan 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 1호 5 51-60 (10 pages)
50mV in 10mV steps and this effect was blocked by the addition of an antagonist. Also Substance P increased transient and spontaneous oscillatory K+ outward currents. The enhanced outward currents were abolished by apamin in dispersed single cells. These results suggest that the depolarization of membrane potential by Substance P activates voltage-dependent Ca2+ channels, which represents an excitatory response in the antral circular muscle and led to an increase in Ca2+ activated K+ currents -
Immunocytochemical Study on the Translocation Mechanism of Glucose Transporters by Insulin
Hah, Jong-Sik, Kim, Ku-Ja 대한생리학회 대한생리학회지 16 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 2 123-138 (16 pages)
glucose transporters between cell surface and intracellular pool was different in adipocytes. The glucose transporter molecules were randomly distributed at the cell surface whereas the molecules at LDM were farmed as clusters. By insulin treatment the number of homogeneous random particles increased at the cell surface whereas the cluster forms decreased at the intracellular storage pools. It suggests that the active molecules needed to be evenly distributed far effective function and that the... -
Ventilatory Responses to Continuous Negative Pressure Breathing(CNPB) in Awake Dogs
Cha, Eun-Jong, Goo, Yong-Sook 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 1호 4 37-50 (14 pages)
a change in sensitivity (p>0.05). These results indicate additive effects of CNPB and CO2 inhalation. The degree of hyperventilation during CNPB at eupnea was estimated to be 63% of that during control ventilation and was significantly greater than zero (p<0.0001), which suggests an alveolar hyperventilation due to CNPB. These results suggest that the mechanical alterations associated with n decrease in lung volume could play an important role in ventilatory control independently of chemical... -
Effect of Capsaicin on Causalgiform Pain in the Rat
Kim, Kwang-Jin, Jeon, Byeong-Hwa, Kim, Won-Sik, Park, Kyung-Ran, Choi, Sae-Jin 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 2호 6 143-150 (8 pages)
that of the right side throughout 8 weeks, and the number of C-fibers decreased by about 30% or more These results suggest that the elevated latency with capsaicin(0.1-1.0%) treatment is due to the inhibition of impulse transmission throughout the primary afferent fiber and the data from 2.0% are due to partial destruction of C-fibers. Therefore, capsaicin concentrations from 0.1% to 1.0% are probably very effective for the treatment of causalgiform pain with almost no destruction of C-fibers. -
Effect of Various Divalent Ions on the Calcium Current of Adrenal Medullary Chromaffin Cells in the Rat
Kim, Jun, Leem, Chae-Hun, Kim, Sang-Jeong 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 2호 2 113-122 (10 pages)
in internal solution). The voltage dependence and the inactivation time course of VDCC in our cells were identical to those of bovine chromaffin cells. A persistent inward current was first activated by depolarizing step pulse from the holding potential (H.P.) of -80 mV to -40 mV, increased to maximum amplitude at around +10 mV, and became smaller with progressively higher depolarizing pulses to reverse at around +60 mV. The inactivation time constant (τ), fitted from the long duration test... -
Characteristics of Synthesized Red Cells Using Bovine Hemoglobin as Oxygen Carrier
Cho, Eng-Haeng, Hah, Jong-Sik, Kim, Ku-Ja 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 4 37-44 (8 pages)
microscope and image analyzer. The function of the hemosome as oxygen carrier was tested by measuring oxygen saturation curve with blood gas analyzer and infusing it into rats. The prepared hemosome was 1.184 + 0.423 μm in diameter and round in shape. P50 value of the hemosome solution was 28 mmHg. The synthesized red cells seem to function as oxygen carrier, because the severely bled rats were prolonged in their life by transfusion of the hemosome solution containing bovine hemoglobin. -
토끼 심방근 및 혈관 평활근에서의 Na+/Ca2+ 교환기전에 관한 연구
김희주(Kim, Hee-Ju), 문형로(Moon, Hyung-Ro), 엄융의(Earm, Yung-E), 호원경(Ho, Won-Kyung) 대한생리학회 대한생리학회지 18 Pages
대한생리학회 대한생리학회지 1988, 제 22권 제 1호 2 13-30 (18 pages)
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사람 적혈구막의 (Na＋+K+)-ATPase/Na+, K+ Pump의 Target Size
하종식(Hah, Jong-Sik), Jung, Chan Y. 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1985, 제 19권 제 1호 2 15-24 (10 pages)
(Na++K+)-ATPase은 α 와 β의 두 subunits로 구성되어 있으며, 분자량이 약 300,000 daltons 정도되는 것으로 보아 α2β2의 형태로 존재할 것으로 알려져 왔다 한편, 사람 적혈구막에 있는 Na+, K+ Pump는 glycolytic enzymes과 complex를 이루고 있으리라는 보고도 있다. 우리는 이 실험에서 in situ상태의 사람 적혈구막(Na++K+)-ATPase의 분자량을 측정하기 위하여, 소위 말하는 ‘Target theory’를 radiation에 의한 ouabain sensitive한 Na+이동과, intact한 cells과 ghosts에서의 ATP가수분해능력의 inactivation data에...
