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전체 선택해제
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본문검색 결과만 보기
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Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid
Goo Jang*, So Gun Hong, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 12 83-89 (7 pages)
Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence was monitored. After 7 days of culturing, the... -
In vitro toxicity of melamine against Tetrahymena pyriformis cells
Zhengfang Wang, Liben Chen, Rashad Al-Kasir, Bo Han* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 5 27-34 (8 pages)
This study assessed the toxicity of melamine against the unicellular eukaryotic system of Tetrahymena (T.) pyriformis exposed to 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine. Cell growth curves of different cultures, the half maximum inhibition concentration (IC50) value of melamine, and morphological changes in cells were obtained via optical and transmission electron microscopic observation. The effects of eleven melamine concentrations, 0.25, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5... -
Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis
Maria Fernanda de M. Costa*, Adriana K. Carmona, Marcio F. M. Alves, Timothy M. Ryan, Helen M. Davies, Garry A. Anderson, Ron F. 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 4 21-25 (5 pages)
Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard"... -
Effect of German chamomile oil application on alleviating atopic dermatitis-like immune alterations in mice
Soon-Hee Lee, Yong Heo, Young-Chul Kim, * 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 6 35-41 (7 pages)
Historically, German chamomile (GC) oil has been used for treatment of skin disorders. BALB/c mice were sensitized twice a week with 100 μL of 1% 2,4-dinitrochlorobenzene (DNCB) and challenged twice the following week with 100 μL of 0.2% DNCB for atopic dermatitis induction. Thereafter, 3% GC oil was applied daily (70 μL, 6 times week) on the dorsal skin for 4 weeks. Saline or jojoba oil was used for the control mice. Blood was collected after second DNCB challenge, and at 2 and 4 weeks after... -
Influence of ascorbic acid on BUN, creatinine, resistive index in canine renal ischemia-reperfusion injury
Jae-il Lee, Myung-jin Kim, Chang-sik Park, Myung-cheol Kim 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 14 79-81 (3 pages)
Renal ischemia as a course of renal transplantation is a common cause of renal dysfunction as renal failure.The purpose of this study was to investigate the influence of ascorbic acid on blood urea nitrogen (BUN), creatinine(Cr)and resistive index (RI)for dog models with renal ischemia-reperfusion (I/R) injury.Renal ischemia was induced on 6 Beagle dogs.The left kidney was exposed to normothermic ischemia for a short period at 30 min followed by reperfusion.On the blood Cr level and RI, there... -
Expression of placenta growth factor mRNA in the rat placenta during mid-late pregnancy
Wan Sung Choi, Gyeong Jae Cho, Chung Kil Won, Phil Ok Koh 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 1 179-183 (5 pages)
The placenta is an essential organ that synthesizes several growth and angiogenic factors for its own growth as well as fetal development. It is known that the placenta growth factor (PlGF)is a member of the vascular endothelial growth factor family and is critical for placental growth and fetal development. However, there is little information regarding the expression pattern and cellular localization of PlGF mRNA in rat placenta during pregnancy. The aim of this study was to define the... -
Mutation and overexpression of p53 as a prognostic factor in canine mammary tumors
Chung-Ho Lee, Wan-Hee Kim, Ji-Hey Lim, Min-Soo Kang, Dae-Yong Kim and Oh-Kyeong Kweon 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 9 63-69 (7 pages)
We concentrated ourselves to evaluate the prognostic significance of the p53 gene mutations, its protein expression and MIB-1 index as a proliferative marker in canine mammary tumors. In the present study, a total of 20 cases were examined, among which there were 5 malignant mixed tumors, 4 mammary gland adenocarcinomas, 1 papillary adenocarcinoma, 8 benign mixed tumors and 2 mammary gland adenomas. Positive immunostaining for p53 with PAb240 antibody was found in 2 benign (20%) and 3 malignant... -
Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae
Sung Jae Shin, Jong Lye Bae, Young Wook Cho, Moon Sik Yang, Dae Hyuk Kim, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 4 225-228 (4 pages)
Actinobacillus pleuropneumoniae is an important primary pathogen in pigs, in which it causes a highly contagious pleuropneumoniae. In our previous study, apxIA gene amplified from A. pleuropneumoniae Korean isolate by PCR with primer designed based on the N- and C-terminal of the toxin was cloned in TA cloning vector and sequenced. The nucleotide sequences of apxIA gene was reported to GeneBank with the accession numbers of AF363361. Identity of the Apx IA from the cloned gene in E. coli was... -
Effects of Combination Dietary Conjugated Linoleic Acid with Vitamin A (Retinol) and Selenium on the Response of the Immunoglobulin Productivity in Mice
Jin-young Kim, Byung-hyun Chung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 16 103-108 (6 pages)
The dietary effect of conjugated linoleic acid (CLA) on the response of the immunoglobulin (serum and tissue) production in Balb/C mice was examined at three doses: 0 %(control), 0.5% and 1.5%. The combination effects of CLA with vitamin ADE or selenium also were investigated. CLA at 0.5% increased serum immunoglobulin A, G, mesenteric lymph node (MHN) and gut luminal IgA (secretory IgA) levels. However, 1.5% CLA decreased SIgG slightly. CLA both alone and combined with vitamin ADE and selenium... -
Mutations of p53 Tumor Suppressor Gene in Spontaneous Canine Mammary Tumors
Chung-Ho Lee and Oh-Kyeong Kweon 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 10 321-325 (5 pages)
Mutation of the p53 tumor suppressor gene has been related in the pathogenesis of numerous human and canine cancers, including breast cancers and mammary tumors. We have investigated exons 5-8 of the p53 gene for mutations in 20 spontaneous canine mammary tumors using polymerase chain reaction (PCR) with direct sequence analysis to evaluate the role of this gene in canine mammary tumorigenesis and analyzed to compare with other clinicopathological parameters including age, histology, stage,...
