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51.

Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin

Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 6 431-437 (7 pages)

Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 저
...
- 3,000원
Monoclonal anti-enrofloxacin antibody was prepared for a direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, and norfloxacin were 5.0, 8.3, 9.7, 21.7, 36.0, and 63.7 ng/mL, respectively. The lowest detectable level of ENR was 0.7 ng/mL in the prepared ELISA system. To validate the...
52.

Evaluation of adjuvant effects of fucoidan for improving vaccine efficacy

Su-Yeon Kim, Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 2 145-150 (6 pages)

Su-Yeon Kim, Hong-Gu Joo 저
...
- 3,000원
Fucoidan is a sulfated polysaccharide derived from brown seaweed, including Fucus vesiculosus. This compound is known to have immunostimulatory effects on various types of immune cells including macrophages and dendritic cells. A recent study described the application of fucoidan as a vaccine adjuvant. Vaccination is regarded as the most efficient prophylactic method for preventing harmful or epidemic diseases. To increase vaccine efficacy, effective adjuvants are needed. In the present study,...
53.

Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)

Seong Bin Park, Kyoung Kwon, In Seok Cha, Ho Bin Jang, Seong Won Nho, Fernand F. Fagutao, Young Kyu Kim, Jong Earn Yu, Tae Sung 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 19 163-166 (4 pages)

Seong Bin Park, Kyoung Kwon, In Seok Cha, Ho Bin Jang, Seong Won Nho, Fernand F. Fagutao, Young Kyu Kim, Jong Earn Yu, Tae Sung 저
...
- 1,500원
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable...
54.

Development and evaluation of a multiplex PCR assay for simultaneous detection of Flavobacterium psychrophilum, Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida in culture fisheries

Ertan Emek Onuk, Alper Ciftci*, Arzu Findik, Yuksel Durmaz 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 9 235-241 (7 pages)

Ertan Emek Onuk, Alper Ciftci*, Arzu Findik, Yuksel Durmaz 저
...
- 3,000원
bacteria were 35 pg for Y. ruckeri and F. psychrophilum and 70 pg for A. salmonicida subsp. salmonicida. It was determined that 15 CFU Y. ruckeri and F. psychrophilum and 30 CFU A. salmonicida subsp. salmonicida could be detected by m-PCR developed using genomic DNA extracted from dilutions of the suspensions. The detection limits in the presence of tissue debris were 125 CFU for Y. ruckeri and F. psychrophilum and 250 CFU for A. salmonicida subsp. salmonicida. In conclusion, we submit that the...
55.

Plasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan

Ji Hyung Kim, Dennis K. Gomez, Toshihiro Nakai, Se Chang Park 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 13 85-87 (3 pages)

Ji Hyung Kim, Dennis K. Gomez, Toshihiro Nakai, Se Chang Park 저
...
- 1,500원
In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm...
56.

In vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida

Tae S. Jung*, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 7 169-176 (8 pages)

Tae S. Jung*, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 저
...
- 3,000원
20 kDa. Differences in glycoprotein were also evident between bacteria cultured in vitro and in vivo. Bacteria cultured in vitro in LMW and HMW bags displayed a single 26 kDa band. Bacteria cultured in the LMW bag in vivo displayed bands at 26 and 27 kDa, while bacteria cultured in vivo in the HMW bag possessed only the 27 kDa band. These bands may represent sialic acid. The significance of the changes observed in the bacterium's structure and antigenicity when cultured in vivo is discussed.
57.

Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro

Tae S. Jung, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 9 255-261 (7 pages)

Tae S. Jung, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 저
...
- 3,000원
bacteria cultured in TSB or GRM. When bacteria were passaged several times on tryptic soya agar prior to culturing in the four different media, only bands at 24 and 47 kDa were recognized, regardless of the medium used to culture the bacteria. It would appear that the molecular weight of Ph. d. subsp. piscicida antigens change in the presence of iron restriction, and sera from sea bass infected with live bacteria are able to detect epitopes on the antigens after this shift in molecular weight.
58.

Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island

Gun Wook Baeck, Ji Hyung Kim, Dennis Kaw Gomez, Se Chang Park 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 9 53-58 (6 pages)

Gun Wook Baeck, Ji Hyung Kim, Dennis Kaw Gomez, Se Chang Park 저
...
- 3,000원
Streptococcus sp.is gram-positive coccus that causes streptococcal infections in fish due to intensification of aquaculture and caused significant economic losses in fish farm industry.A streptococcal infection occurred from cultured diseased olive flounder (Paralichthys olivaceus )in May, 2005 at a fish farm in Jeju Island, Korea.The diseased flounder exhibited bilateral exophthalmic eyes and rotten gills;water temperature was 16~18oC when samples were collected.Of the 22 fish samples...
59.

Isolation and characterization of Brucella abortus isolates from wildlife species in South Korea

Quang Lam Truong , Kiju Kim , Jong-taek Kim , Moon Her , Suk-chan Jung , Tae-wook Hahn 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 3호 3 147-153 (7 pages)

Quang Lam Truong , Kiju Kim , Jong-taek Kim , Moon Her , Suk-chan Jung , Tae-wook Hahn 저
...
- 3,000원
60.

Isolation and molecular identification of Vibrio harveyi from the look down fish (Selene vomer)

Jin Woo Jun , Young Ran Lee , Sib Sankar Giri , Hyoun Joong Kim , Sae Kil Yun , Cheng Chi , Sang Guen Kim , Jeong Rack K 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 3 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 2호 12 125-127 (3 pages)

Jin Woo Jun , Young Ran Lee , Sib Sankar Giri , Hyoun Joong Kim , Sae Kil Yun , Cheng Chi , Sang Guen Kim , Jeong Rack K 저
...
- 1,500원

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