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Further Characterization of Bombesin Like Immunoreactivities from the Skin of Korean Fire-bellied Toad, Bombina orientalis
HyeokYilKwon, HyungSeoPark, MooHoWon, YunLyulLee, HyoungJinPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 12 637-644 (8 pages)
Previously, we have isolated authentic bombesin and another bombesin like peptide named bombesin like immunoreactivity (BLI)-K2 from the skin of Korean fire-bellied toad, Bombina orientalis. In the present study, we have newly purified three heterogeneous forms of BLI named BLI-K3, BLI-K4, and BLI-K5 from side fractions obtained in previous isolation of bombesin like peptide. The BLIs were separated into five peaks on a column of C18 preparative HPLC. Among them, three minor peaks containing... -
Protective Mechanism of Nitric Oxide and Mucus against Ischemia/ Reperfusion-Induced Gastric Mucosal Injury
HyeyoungKim, KwangsooNam, KyungHwanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.4 13 511-519 (9 pages)
This study investigated the role of nitric oxide on the oxidative damage in gastric mucosa of rats which received ischemia/reperfusion and its relation to mucus. Nitric oxide synthesis modulators such as L-arginine and NG-nitro-L-arginine methyl ester, and sodium nitroprusside, a nitric oxide donor, were injected intraperitoneally to the rats 30 min prior to ischemia/reperfusion which was induced by clamping the celiac artery and the superior mesenteric artery for 30 min and reperfusion for 1... -
Cromakalim Blocks Membrane Phosphoinositide Activated Signals in the Guinea Pig Lung Mast Cells Stimulated with Antigen-Antibody Reactions
JaiYoulRo, JiYoungKim, KyungHwanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.2 14 251-260 (10 pages)
Cromakalim (BRL 34915), known as an airway smooth muscle relaxant, inhibited the releases of mediators in the antigen-induced mast cell activation. It has been suggested that cromakalim, in part, inhibited mediator releases by inhibiting the initial increase of 1,2-diacylglycerol (DAG) produced by the activation of the other phospholipase system which is different from phosphatidylcholine-phospholipase D pathway. The aim of this study is to further examine the inhibitory mechanism of cromakalim... -
The Inhibitory Mechanism of Aloe Glycoprotein (NY945) on the Mediator Release in the Guinea Pig Lung Mast Cell Activated with Antigen-Antibody Complexes
JaiYoulRo, ByungChulLee, MyungHeeChung, SeungKiLee, ChungKiSung, KyungHwanKim, YoungInPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 13 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.1 15 119-131 (13 pages)
It has been reported that the g1ycoprotein extracted from A10e has strong anti-inflammatory response, However, there has been no research report yet about the effect of A10e on allergic hypersensitivity reactivity, By using guinea pig 1ung mast cells, this study aimed to examine the effects of A10e g1ycoprotein (NY945) on the mediator re1eases caused by mast cell activation, and a1so aimed to assess the effects of NY945 on the mechanism of mediator re1eases in the mast cell activation. We... -
Effects of Ginsenosides on the Mechanism of Histamine Release in the Guinea Pig Lung Mast Cells Activated by Specific Antigen- Antibody Reactions
JaiYoulRo, YoungSooAhn, KyungHwanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.4 11 445-456 (12 pages)
We previously reported that some components of ginsenosides decreased mediator releases evoked by the activation of mast cells with specific antigen-antibody reactions. This study aimed to assess the effects of ginsenosides (Rb2, Re) on the mechanism of histamine release in the mast cell activation. We partially purified guinea pig lung mast cells by using enzyme digestion, the rough and the discontinuous percoll density gradient method. Mast cells were sensitized with IgG1 and challenged with... -
[3H]Ryanodine Binding Sites of SR Vesicles of the Chicken Pectoral Muscle
Hyo-YungYun, Jong-RyeJeon, Jang-HeeHong, Gang-MinHur, Jae-HeunLee, Jeong-HoSeok* 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.4 4 377-384 (8 pages)
To investigate the properties of ryanodine binding sites of the bird skeletal SR vesicles, SDS PAGE, purification of RyR, and [3H]ryanodine binding study were carried out in the SR vesicles prepared from the chicken pectoral muscle. The chicken SR vesicles have two high molecular weight (HMW) protein bands as in eel SR vesicles on SDS PAGE. The HMW bands on SDS PAGE were found in the [3H] ryanodine peak fraction (Fr3-5) obtained from the purification step of the ryanodine receptor protein. Bmax... -
Reduction of TNFα-induced Oxidative DNA Damage Product, 8-Hydroxy-2′-Deoxyguanosine, in L929 Cells Stably Transfected with Small Heat Shock Protein
Young-MeePark, Eun-MiChoi 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.2 11 209-219 (11 pages)
Previous studies have demonstrated that oxidative stress involving generation of reactive oxygen species (ROS) is responsible for the cytotoxic action of TNFα. Protective effect of small heat shock proteins (small HSP) against diverse oxidative stress conditions has been suggeted. Although overexpression of small hsp was shown to provide an enhanced survival of TNFα-sensitive cells when challenged with TNFα, neither the nature of TNFα-induced cytotoxicity nor the protective mechanism of... -
백서 뇌 별아교세포에서 LipopolysaccharideI에 의한 Nitric Oxide 생성기전
소광, 박래길, 이상렬, 박민철 대한신경정신의학회 신경정신의학 13 Pages
대한신경정신의학회 신경정신의학 1999, 제 38권 제 4호 20 881-893 (13 pages)
연구목적: Nitric oxide(NO)는 뇌졸중 및 여러 가지 신경변성 질환의 병리생리에 중요한 역할을 한다. 본 연구는 백서 뇌로부터 분리한 별아교세포에서 세균내독소 lipopolysaccharide(LPS)에 의한 NO 생성과정이 MAP kinase cascades와 전사 활성인자 NF-kB에 의해 중재됨을 밝히고자 하는 것이다. 방 법: NO 생성은 Greese’s reagents와 Western blot analysis를 사용하여 측정하였고 Erk, JNK1, p38은 in vitro immunecomplex kinase assay로 측정했다. NF-kB의 전사적 활성은 electrophoretic mobility shift assay로 결정하였다.... -
흰쥐 해마에서 경련에 의해 발현 유도된 MKP-1에 의한 MAPK 활성 조절
유범희, 강웅구, 안용민, 정선주, 전송희, 박주배, 김용식 대한신경정신의학회 신경정신의학 8 Pages
대한신경정신의학회 신경정신의학 1999, 제 38권 제 4호 19 873-880 (8 pages)
연구목적: 전기경련 충격(Electroconvulsive shock, ECS) 및 카이닌산(kainic acid)에 의한 경련은 흰쥐 해마에서 mitogen activated protein kinase(MAPK)를 활성화시키며, 동시에 MAPK 불활성화 효소인 MAPK phosphatase- 1(MKP-1)의 발현을 일으킨다. 이 연구의 목적은 경련에 의해 발현된 MKP-1이 역시 경련에 의해 활성화된 MAPK의 불활성화에 관여하는지를 알아보고자 하는 것이다. 방 법: 흰쥐에 ECS를 가하여 해마에서 MKP-1 발현을 일으킨 뒤 다시 ECS를 가하고, 두 번째 ECS에 의한 MAPK의 일시적 활성화가 MKP-1의... -
Dopamine Transporter Gene의 다형성과 알코올 의존증의 관련
최윤정, 방승규, 김정진, 도규영, 백인호 대한신경정신의학회 신경정신의학 8 Pages
대한신경정신의학회 신경정신의학 1999, 제 38권 제 4호 14 826-833 (8 pages)
증폭하였다. 증폭된 산물을 15% polyacrylamide gel에서 전기영동하 여 나타나는 띠의 크기에 따라 유전자형을 구분하였다. 자료의 통계적 처리는 Fisher’s exact test, oneway ANOVA를 사용하였다. 결 과: 환자군과 정상대조군의 유전자형은 모두 Hardy-Weinberg equilibrium의 예상치와 유의한 차이가 없었 다. 대립유전자는 7 repeats, 9 repeats, 10 repeats, 11 repeats의 4가지 종류로 구분되었고, 유전자형은 7/7 repeats, 7/10 repeats, 9/10 repeats, 10/10 repeats, 10/11 repeats의 5가지 형태를 보였다. 환자군과 대...


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