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61.

Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil

Rosiane Nascimento Alves*, Susana Elisa Rieck, Carlos Ueira-Vieira, Marcelo Bahia Labruna, Marcelo Emílio Beletti 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 10 241-248 (8 pages)

Rosiane Nascimento Alves*, Susana Elisa Rieck, Carlos Ueira-Vieira, Marcelo Bahia Labruna, Marcelo Emílio Beletti 저
...
- 3,000원
Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlândia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28...
62.

Differential expression of thymic DNA repair genes in low-dose-rate irradiated AKR/J mice

Jin Jong Bong, Yu Mi Kang, Suk Chul Shin, Seung Jin Choi, Kyung Mi Lee, Hee Sun Kim 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 6 271-279 (9 pages)

Jin Jong Bong, Yu Mi Kang, Suk Chul Shin, Seung Jin Choi, Kyung Mi Lee, Hee Sun Kim 저
...
- 3,000원
We previously determined that AKR/J mice housed in a low-dose-rate (LDR) (Cs, 0.7 mGy/h, 2.1 Gy) γ-irradiation facility developed less spontaneous thymic lymphoma and survived longer than those receiving sham or high-dose-rate (HDR) (Cs, 0.8 Gy/min, 4.5 Gy) radiation. Interestingly, histopathological analysis showed a mild lymphomagenesis in the thymus of LDR-irradiated mice. Therefore, in this study, we investigated whether LDR irradiation could trigger the expression of thymic genes involved...
63.

Sporozoite proteome analysis of Cryptosporidium parvum by one-dimensional SDS-PAGE and liquid chromatography tandem mass spectrometry

AMAM Zonaed Siddiki* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 1 107-114 (8 pages)

AMAM Zonaed Siddiki* 저
...
- 3,000원
Despite the development of new technologies, new challenges still remain for large scale proteomic profiling when dealing with complex biological mixtures. Fractionation prior to liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis is usually the preferred method to reduce the complexity of any biological sample. In this study, a gel LC-MS/MS approach was used to explore the stage specific proteome of Cryptosporidium (C.) parvum. To accomplish this, the sporozoite protein of C....
64.

Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times

Miao Zhang, Zhenhua Yue, Zhijun Liu, Ali Islam, Buriro Rehana, Shu Tang, Endong Bao*, Jorg Hartung 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 6 253-259 (7 pages)

Miao Zhang, Zhenhua Yue, Zhijun Liu, Ali Islam, Buriro Rehana, Shu Tang, Endong Bao*, Jorg Hartung 저
...
- 3,000원
The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70...
65.

In vitro toxicity of melamine against Tetrahymena pyriformis cells

Zhengfang Wang, Liben Chen, Rashad Al-Kasir, Bo Han* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 5 27-34 (8 pages)

Zhengfang Wang, Liben Chen, Rashad Al-Kasir, Bo Han* 저
...
- 3,000원
This study assessed the toxicity of melamine against the unicellular eukaryotic system of Tetrahymena (T.) pyriformis exposed to 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine. Cell growth curves of different cultures, the half maximum inhibition concentration (IC50) value of melamine, and morphological changes in cells were obtained via optical and transmission electron microscopic observation. The effects of eleven melamine concentrations, 0.25, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5...
66.

Charting the proteome of Cryptosporidium parvum sporozoites using sequence similarity-based BLAST searching

A.M.A.M.Z. Siddiki*, Jonathan M. Wastling 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 5 203-210 (8 pages)

A.M.A.M.Z. Siddiki*, Jonathan M. Wastling 저
...
- 3,000원
Cryptosporidium (C.) spp. are important zoonotic parasites causing widespread diarrhoeal disease in man and animals. The recent release of the complete genome sequences for C. parvum and C. hominis has facilitated the comprehensive global proteome analysis of these opportunistic pathogens. The well-known approach for mass spectrometry (MS) based data analysis using the BLAST tool (MS BLAST) is a database search protocol for identifying unknown proteins by sequence similarity to homologous...
67.

Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication

Eun-Joo Kim, Sang-Hee Jeong, Joon-Hyoung Cho, Hyun-Ok Ku, Hyun-Mi Pyo, Hwan-Goo Kang, Kyoung-Ho Choi 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 5 257-266 (10 pages)

Eun-Joo Kim, Sang-Hee Jeong, Joon-Hyoung Cho, Hyun-Ok Ku, Hyun-Mi Pyo, Hwan-Goo Kang, Kyoung-Ho Choi 저
...
- 3,000원
This study aimed to discover potential biomarkers for dioxynivalenol (DON) intoxication. B6C3F1 male mice were orally exposed to 0.83, 2.5 and 7.5 mg/kg body weight (bw) DON for 8 days and the differential protein expressions in their blood plasma were determined by SELDI - Time-of-Flight/ Mass Spectrometry (TOF/MS) and the immunoglobulins (Igs) G, A, M and E in the serum were investigated. 11.7 kDa protein was significantly highly expressed according to DON administration and this protein was...
68.

Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes

Yong-Seung Shin, Eung-Goo Lee, Tae-Sung Jung 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 11 157-160 (4 pages)

Yong-Seung Shin, Eung-Goo Lee, Tae-Sung Jung 저
...
- 1,500원
The present study was attempted to compare the Neospora caninum (N. caninum) antigenic bands recognized by different bovine immunoglobulin classes. A total 10, 5, 2, and 6 antigenic bands were exhibited on immunoblot profiles against bovine IgM, IgE, IgA, and IgG, respectively. A 46 kDa band was probed as a common antigenic band except IgA; 69 kDa band was bovine IgM and IgE; 33, 37, 55, and 79 kDa bands were bovine IgM and IgG; 72 kDa band was found IgM and IgA profiles. Based on the analysis,...
69.

Application of biotechnological tools for coccidia vaccine development

Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 1 279-288 (10 pages)

Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 저
...
- 3,000원
Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and...
70.

Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 8 139-145 (7 pages)

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 저
...
- 3,000원
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the N. caninum proteome. Protein sample preparation was carried out by first conducting sonication, followed by adding lysis buffer containing 7M urea plus 2M thiourea to the purified tachyzoites in order to complete disruption. A total of 335 differentially expressed...

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