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The Road to RNA Silencing is Paved with Plant-Virus Interactions
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 1 197-206 (10 pages)
had a large impact on biology in general, as well as on our understanding of plantpathogen interactions, especially interactions between plants and viruses. While most of what we know about the mechanism of RNA silencing was deduced in the last 12 years, many of the interactions between plants and viruses, as well as virus-virus interactions in plants, which we now know are manifestations of RNA silencing, were the subject of decades of work from numerous laboratories. These laboratories were... -
Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)
Rice stripe virus (RSV) suggested possible genetic reassortment of RNA segments, but whether this RNA variation contributed to the recent RSV outbreaks in Korea is yet unclear. To further clarify these RSVRNA segment variations, we developed a reverse transcription- polymerase reaction/restriction enzyme (RTPCR/ RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV RNA 1-4 subtypes, respectively. Our RT-PCR/RE results provided... -
Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 14 286-288 (3 pages)
method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR. -
식물 RNA 바이러스의 진화와 병저항성 극복 기작
김명휘, 권선정, 서장균 한국식물병리학회 식물병연구 12 Pages
한국식물병리학회 식물병연구 2021, 27권 4호 2 137-148 (12 pages)
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딸기바이러스 진단을 위한 최적의 RNA 추출 방법 및 주요 7종 딸기바이 러스의 진단법 개발
권선정, 윤주연, 조인숙, 정봉남 한국식물병리학회 식물병연구 9 Pages
한국식물병리학회 식물병연구 2020, 26권 3호 5 170-178 (9 pages)
추출효율 비교하였다. 바이러스 진단을 통한 RNA 추출효율을 분석하기 위해 SMoV 감염주인 미홍 딸기 품종을 이용하여 다양한 단계에서 잎조직으로부터 RNA를 추출하고 바이러스 진단을 수행하였다. 식물 RNA 추출 방법 가운데 상업용으로 판매되는 RNeasy plant mini kit (Qiagen)를 이용하는 경우 본 연구에서 살펴본 onestep 또는 two-step RT-PCR 방법과 무관하게 SMoV의 검출이잘 되었다. 또한, 딸기 우량묘의 바이러스 검정에 대한 신뢰있는진단방법을 구축하기 위해 주요 딸기 바이러스인 strawberry mild yellow edge virus (SMYE... -
Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants
Chung Youl Park, Hyun-Geun Min, Hong-Kyu Lee, Rameswor Maharjan, Youngnam Yoon, Su-Heon Lee 한국식물병리학회 식물병연구 5 Pages
한국식물병리학회 식물병연구 2018, 24권 4호 15 348-352 (5 pages)
multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay was developed for the detection of Clover yellow vein virus (ClYVV), Peanut mottle virus (PeMoV), and Tomato spotted wilt virus (TSWV), which were recently reported to infect soybean and azuki bean in Korea. Species-specific primer sets were designed for the detection of each virus, and their specificity and sensitivity were tested using mixed primer sets. From among the designed primer sets, two combinations were selected... -
A New Double-Stranded RNA Mycovirus from Pleurotus ostreatus (ASI 2504)
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2006, 22권 1호 11 68-74 (7 pages)
kb in sizes) were isolated by ultracentrifugation in sucrose gradients. Western analysis of protein extracted purified viruses with anti-virus polyclonal antibody confirmed that viruses have two specific proteins (36 and 68 kDa). Computer analysis of 2.0 kb segment shows that high sequence identity with RNA-dependent RNA polymerase (RdRp) of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis, OMDV was most related to Pleurotus ostreatus virus 1.


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