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Downregulation of cellular prion protein inhibited the proliferation and invasion and induced apoptosis of Marek’s disease virus-transformed avian T cells
Xuerui Wan, Runxia Yang, Guilin Liu, Manling Zhu, Tianliang Zhang, Lei Liu, Run Wu* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 6 171-178 (8 pages)
mammals, the correlated functions of chicken PrP (chPrP) remain unknown. In this study, stable chPrP-downregulated Marek’s disease (MD) virus-transformed avian T cells (MSB1-SiRNA-3) were established by introducing short interfering RNA (SiRNA) targeting chicken prion protein genes. We found that downregulation of chPrPinhibits proliferation, invasion, and migration, and induces G1 cell cycle phase arrest and apoptosis of MSB1-SiRNA-3 cells compared with Marek’s disease virus-transformed avian... -
Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes
Masoumeh Firouzamandi, Hassan Moeini, Davood Hosseini, Mohd Hair Bejo, Abdul Rahman Omar, Parvaneh Mehrbod, Aini Ideris 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 3 21-26 (6 pages)
The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant... -
Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV
Kiarash Roohani, Sheau Wei Tan, Swee Keong Yeap, Aini Ideris, Mohd Hair Bejo, Abdul Rahman Omar 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 8 447-457 (11 pages)
A Newcastle disease virus (NDV) isolate designated IBS002 was isolated from a commercial broiler farm in Malaysia. The virus was characterised as a virulent strain based on the multiple basic amino acid motif of the fusion (F) cleavage site RRRKGF and length of the C-terminus extension of the hemagglutinin-neuraminidase (HN) gene. Furthermore, IBS002 was classified as a velogenic NDV with mean death time (MDT) of 51.2 h and intracerebral pathogenicity index (ICPI) of 1.76. A genetic distance... -
Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers
Huayuan Lin, Qiqi Huang, Xiaoquan Guo, Ping Liu, Weilian Liu, Yuelong Zou, Shuliang Zhu, Guangfu Deng, Jun Kuang, Caiying Zhang, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 5 423-429 (7 pages)
assess relationships between xanthine oxidase (XOD) and nephropathogenic infectious bronchitis virus (NIBV) infection, 240 growing layers (35 days old) were randomly divided into two groups (infected and control) of 120 chickens each. Each chicken in the control and infected group was intranasally inoculated with 0.2 mL sterile physiological saline and virus, respectively, after which serum antioxidant parameters and renal XOD mRNA expression in growing layers were evaluated at 8, 15 and 22 days... -
Establishment and evaluation of a murine ανβ3-integrin- expressing cell line with increased susceptibility to Foot- and-mouth disease virus
Wei Zhang, Kaiqi Lian, Fan Yang, Yang Yang, Zhijian Zhu, Zixiang Zhu, Weijun Cao, Ruoqing Mao, Ye Jin, Jijun He, Jianhong Guo, X 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 3 265-272 (8 pages)
and IFA were used to detect the replication levels of Foot-and-mouth disease virus (FMDV) in the CHO-677-mανβ3 cell line. After infection with FMDV/O/ZK/93, the cell line showed a significant increase in viral RNA and protein compared with CHO-677 cells. These findings suggest that we successfully established a stable ανβ3-receptor-expressing cell line with increased susceptibility to FMDV. This cell line will be very useful for further investigation of ανβ3 integrin, and as a cell... -
A study of experimental autoimmune encephalomyelitis in dogs as a disease model for canine necrotizing encephalitis
Jong-Hyun Moon, Hae-Won Jung, Hee-Chun Lee, Joon-Hyeok Jeon, Na-Hyun Kim, Jung-Hyang Sur, Jeongim Ha, Dong-In Jung 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 10 203-211 (9 pages)
In the present study, the use of dogs with experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs were included in this study. Canine forebrain tissues (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund’s complete adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra.... -
Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 7 511-517 (7 pages)
of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold.... -
Dietary germanium biotite supplementation enhances the induction of antibody responses to foot-and-mouth disease virus vaccine in pigs
Jin-A Lee, Bock-Gie Jung, Myunghwan Jung, Tae-Hoon Kim, Han Sang Yoo, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 15 443-447 (5 pages)
We evaluated the potential ability of germanium biotite (GB) to stimulate the production of antibodies specific for foot-and-mouth disease virus (FMDV). To this aim, we measured the total FMDV-specific antibody responses and IgM production after vaccination against FMD both experimentally and in the field. GB supplementation with FMDV vaccination stimulated the production of anti-FMDV antibodies, and effectively increased IFN-γ and TNF-α levels. These results suggest that GB may be a novel... -
A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
Yao-zhong Ding, Jian-hua Zhou, Li-na Ma, Yan-ni Qi, Gang Wei, Jie Zhang, *, Yong-guang Zhang* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 11 423-426 (4 pages)
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for... -
Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus
Lishan Lv, Xiaoming Li, Genmei Liu, Ran Li, Qiliang Liu, Huifang Shen, Wei Wang, Chunyi Xue, Yongchang Cao* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 6 209-216 (8 pages)
Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the spread of IBV, but currently available inactivated and attenuated virus vaccines have some disadvantages. We developed a chimeric virus-like particle (VLP)-based candidate vaccine for IBV protection. The chimeric VLP was composed of matrix 1 protein from avian influenza H5N1 virus and a...


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