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Bovine adenovirus type 3 virions cannot be rescued in vivo after full-length viral genome transfection in the absence of detectable polypeptide IX
Peng Zhang, Qinghong Xue, Jing Ma, Jingjing Ren, Shuili Xia, Lu Zhang, Wenbin Wang, Suresh K. Tikoo, Enqi Du 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 12 217-227 (11 pages)
a more comprehensive description of BAdV3 biology is essential. In this study, we focused on the role of pIX in BAdV3 virion rescue after full-length BAdV3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions, which suggested that pIX was essential for the rescue of BAdV3 containing a full-length genome. Moreover, through transfection of a panel of pIX mutant BAdV3 genomes, we observed that the conserved N-terminus and the putative... -
The Transfection of Caldesmon DNA into Primary Cultured Rat Aortic Vascular Smooth Muscle
WoongChoiHee-YulAhn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.6 8 597-603 (7 pages)
Caldesmon (CaD), one of microfilament-associated proteins, plays a key role in microfilament assembly in mitosis. We have investigated the effects of overexpression of the high molecular weight isoform of CaD (h-CaD) on the physiology of vascular smooth muscle cells (VSMCs). Rat aortic VSMCs were stably transfected with plasmids carrying a full length human h-CaD cDNA under control of cytomegalovirus promoter. The majority of the overexpressed h-CaD appears to be localized predominantly on... -
Transformation of Rabbit Proximal Tubule Cells by Strontium Phosphate Transfection with a Plasmid Containing SV4O Early Region Genes
Han, Ho-Jae, Taub, Mary L., 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 14 233-240 (8 pages)
In this study, it was investigated whether immortalized proximal tubule cells transformed with pRSVT could survive through the numerous passages. Results were as follows: 1. The cells transfected with pRSVT formed rapidly growing, multilayered colonies within 2 weeks in a hormone defined medium. Domes were also observed in some of the cultures. 2. r-glutamyl transpeptidase activity was equivalent to that observed in primary renal proximal tubule cell cultures. 3. Transformed cells with pRSVT... -
안정성 및 Transfection 효율이 우수한 양이온성 리포좀 유전자 전달시스템의 개발
김경미, 남방현, 손동환, Kim. Kyoung-Mi, Nam. Bang-Hyun, Sohn. Dong-Hwan 한국약제학회 藥劑學會誌 6 Pages
한국약제학회 藥劑學會誌 1998, Vol.28 No.2 93-98 (6 pages)
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Enhancing Transfection Efficiency Using Polyethylene Glycol Grafted Polyethylenimine and Fusogenic Peptide
Lee. Haeshin, Jeong. Ji-Hoon, Lee. Je-Hoon, Park. Tae-Gwan 한국생물공학회 Biotechnology and bioprocess engineering 5 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2001, Vol.6 No.4 269-273 (5 pages)
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Culture of Endothelial Cells by Transfection with Plasmid Harboring Vascular Endothelial Growth Factor
Chang. Sungjaae, Sohn. Insook, Park. Inchul, Sohn. Youngsook, Hong. Seokil, Choe. Teaboo 한국생물공학회 Biotechnology and bioprocess engineering 4 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2000, Vol.5 No.2 106-109 (4 pages)
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A Reliable Protocol for transfection of mature primary hippocampal neurons using a neuron-glia co-culture system
이현숙, 조선정, 정용욱, 진익렬, 문일수, Lee. Hyun-Sook, Cho. Sun-Jung, Jung. Yong-Wook, Jin. Ing-Nyol, Moon. Il-Soo 한국생명과학회 생명과학회지 6 Pages
한국생명과학회 생명과학회지 2007, Vol.17 No.2 198-203 (6 pages)
신경세포는 어린 신경세포나 다른 세포종에 비하여 형질전환이 어렵고 쉽게 죽는다. 본 연구에서는 Clontech사의 $CalPhos^{TM}$ Mammalian Transfection 방법을 수정하여 성숙한 신경세포를 효율적으로 형질전환할 수 있는 방법을 고안하였다. 대뇌 신경교세포를 DMEM/10% 말혈청에서 70-80% confluence까지 키우고 배지를 혈청이 첨가되지 않은 Neurobasal/Ara-C로 바꾸어 주어 더 이상 신경교세포가 분열하지 않게 한 다음, 여기에 E19 해마신경세포를 접종하여 배양하였다. $DNA/Ca^{2+}$-인산 침전물은 Clontech사의 $CalPhos^{TM}$... -
Transfection Property of a New Cholesterol-Based Cationic Lipid Containing Tri-2-Hydroxyethylamine as Gene Delivery Vehicle
Kim. Bieong-Kil, Doh. Kyung-Oh, Hwang. Guen-Bae, Seu. Young-Bae 한국미생물생명공학회 Journal of microbiology and biotechnology 6 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2012, Vol.22 No.6 866-871 (6 pages)
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Analysis of Transgene Intergration Efficiency into Porcine Fetal Fibroblast using Different Transfection Methods
Kim. Baek-Chul, Kim. Hong-Rye, Kim. Myung-Yoon, Park. Chang-Sik, Jin. Dong-Il 한국동물번식학회 Reproductive & developmental biology 5 Pages
한국동물번식학회 Reproductive & developmental biology 2009, Vol.33 No.2 113-117 (5 pages)


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