자료유형
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Comparison of Properties of Polyclonal Anti-N-Acetylchitooligosaccharides and Anti-Chitooligosaccharides Antibodies Produced for ELISA
Shim. Youn-Young, Shon. Dong-Hwa, Kwak. Bo-Yeon, Yu. Jae-Hoon, Chee. Kew-Mahn 한국미생물생명공학회 Journal of microbiology and biotechnology 7 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2004, Vol.14 No.4 686-692 (7 pages)
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Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43
Hyun-Kyoung Lee, Byoung-Hee Lee, Seung-Hyeok Seok, Min-Won Baek, Hui-Young Lee, Dong-Jae Kim, Yi-Rang Na, Kyoung-Jin Noh, Sung-H 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 12 165-167 (3 pages)
for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43. -
Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules
William C. Davis*, Mary Jo Hamilton 대한수의학회 Journal of Veterinary Science 16 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 8 51-66 (16 pages)
Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for reactivity with rabbit LDM yielded 11 mAbs that recognize conserved epitopes on rabbit LDM orthologues and multiple mAbs that recognize epitopes expressed on the major histocompatibility class I or class II molecules. Screening of mAbs submitted to the Animal Homologues Section of... -
Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)
Geewook Shin, Hyungjun Lee, K. J. Palaksha, Youngrim Kim, Eunyoung Lee, Yongseung Shin, Eunggoo Lee, Kyungdae Park, Taesung Jung 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 14 293-295 (3 pages)
The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG affinity columns. These three different ligands were found to possess high affinity for black rockfish serum Ig. All of the Igs purified eluted at only 0.46 M NaCl concentration in anion exchange column chromatography and consisted of two bands at 70 kDa and 25 kDa in SDS-PAGE;... -
Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine
Dong-Kun Yang, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Kyung-Woo Lee, Cheong-Up Choi, Chang-Hee Kweon 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 10 271-275 (5 pages)
The Japanese encephalitis virus (JEV) is one of causative agents of reproductive failure in pregnant sows. An indirect enzyme-linked immunosorbent assay (I-ELISA) was examined for its potential use in the rapid monitoring of the JEV, and the results were compared with those from the hemagglutination inhibition (HI) and serum neutralization (SN) tests. The comparative analysis showed that the results of I-ELISA showed a significant correlation with the conventional HI (r = 0.867) and SN tests (r... -
Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies
Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 6 41-46 (6 pages)
Five monoclonal antibodies (MAbs) and chicken immunoglobulin (IgY) were developed by immunizing with flagella purified from Listeria monocytogenes 4b and the five MAbs have been confirmed to be specific against three different epitopes of flagellin. The antibodies showed specific reaction to Listeria genus and no cross-reactivity with other bacteria tested in this experiment including E.coli O157:H7 and Salmonella enteritidis. Sandwich enzyme-linked immunosorbent assays (ELISA) using the MAbs... -
Production and Characterization of Specific Antibodies to Bombesin
Kwon, Hyeok-Yil, Lee, Yun-Lyul, Park, Hyoung-Jin 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 1호 9 91-98 (8 pages)
In order to produce a specific bombesin antiserum far very sensitive radioimmunoassay, synthetic [lys3]-bombesin conjugated to bovine serum albumin was subcutaneously injected into guinea pigs. The conjugation was performed using either carbodiimide or gIutaraldehyde as a coupling agent. The antisera were characterized by analysis of Scatchard and Sips plots. The antiserum LBE 2G/2 raised by repeat injection of the immunogen conjugated with carbodiimide showed the titer of 1 : 188,000, very low... -
Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 9 452-459 (8 pages)
expressed 41 kDa protein was purified under denaturing condition by affinity chromatography, reconfirmed by Western blotting and plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) before being used as a recombinant antigen to raise polyclonal antibodies in rabbits. Purified anti-GFLV MP immunoglobulines (IgGs) and conjugated IgGs detected the expressed MP and GFLV virions in infected grapevines when used in PTA-ELISA, double antibody sandwich-ELISA, and Western blotting. This is... -
Generation of Antibodies Against Rice stripe virus Proteins Based on Recombinant Proteins and Synthetic Polypeptides
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 7 37-43 (7 pages)
CP and NCP were very effective to detect RSV in both RSV infected rice and weed plants. However, antibodies against NS3 and NSvc4 showed weak specific bands as well as strong non-specific background due to the difference of viral proteins produced in the infected leaves. In summary, the antibodies generated against RSV proteins produced in this study will be useful for various assays such as for RSV diagnostic detection, immunoprecipitation, protein purification, and western blot analysis.
