The effect of the pulsation on the particle hold-up in a pulsed wetted column has been studied theoretically for the case of laminar region and experimentally for the cases of transient and turbulent regions. The experiment has been carried out by employing water as fluid medium and solid particles. The pulsation has been given by the reciprocating motion of a piston. The results of the investigation show that the pulsation would not give apparent effect on the particle hold-up but would increase the total travelling length of the particle.