전보(前報)에서 분리(分離) 선정(選定)한 균주(菌株)로 밀기울 및 밀기울 20% 함유(含有) 왕겨에 배양(培養)하여 만든 발효사료(醱酵飼料)를 산란계(産卵鷄)의 기본사료(基本飼料)에 10% 첨가(添加)하여 사양시험(館養試驗)을 하고 또한 조(粗) cellulase 제(劑) 제조(製造)에 대(對)한 검토(檢討)와 이들균(菌)의 동정(同定)을 한 결과(結果)는 다음과 같다. 1. 선정(選定)한 4균주(菌株)는 MC-9 : Aspergillus niger van Tieghem MC-10 : Aspergillus flavus Link MC-53 : Aspergillus penicilloides Spegazzini MC-61 : Aspergillus niger mut. Schiemanni n. comb. 등(等)과 근연(近緣)의 균주(菌株)이었다. 2. 선정(選定) 균주(菌株)의 밀기울 배양(培養)에서 얻은 조효소제(粗酵素劑) 의 수량(收量)은 배양풍건물(培養風乾物)에 대(對)하여 MC-9에서는 2.9 %, MC-10 : 3.9%, MC-53 : 6.4%, MC-61 : 2.6 % 등(等)이었으며 이들 조효소제(粗酵素劑)의 역가(力價)도 밀기울 배양물(培養物) 추출(抽出) 효소액(酵素液)에서와 같이 MC-9의 것이 가장 강(强) 하였다. 3. 사양시험(飼養試驗) 결과(結果), 체중변화(體重變化)에 있어서는 대조군(對照群) 에 비(比)하여 유의차(有意差)가 없었으나 산란율(産卵率)은 MC-9 및 MC-61의 발효사료(醱酵飼料) 급이군(給餌群)에서 P<0.05 수준(水準)의 증가(增加) 유의차(有意差)를 나타내었다.
The strains selected in the previous paper were identified according to the manual of Aspergilli by Thom and Raper, and the preparation of the crude enzymes with the strains were investigated. The fermented fodders were made with wheat bran and chaffs containing 20% wheat bran by the strains, and the feeding experiments concerned was conducted. The results obtained are as follows: 1. The selected strains were identified as below; MC-9 was a similar strain of Aspergillus niger van Tieghem, MC-10 was a similar strain of Aspergillus flavus Link, MC-53 was a similar strain of Aspergillus penicilloides Spegazzini and MC-61 was a similar strain of Aspergillus niger mut Schiemanni n. comb. 2. Percentages of crude enzymes obtained from the dried matters of the wheat bran cultures were MC-9: 2.9%, MC-10: 3.9%, MC-53: 6.4% and MC-61:2.6%. And the cellulase activity of MC-9 was the most active among the crude enzymes as the same as the filtrates of wheat bran cultures. 3. As the results of the feeding experiments, changes of body weights showed no trends to be significant as compared with the control group, and the groups of MC-9 and MC-61 showed the increasing significance with P<0.05 in egg-laying rate.
