- Nocardia Sp.에 의한 ${Delta}^4$-3-Ketosteroid 류의 산화
- ㆍ 저자명
- 김옥희,이상섭,Kim. Ok-Hee,Lee. Sang-Sup
- ㆍ 간행물명
- 한국생화학회지
- ㆍ 권/호정보
- 1974년|7권 1호|pp.67-74 (8 pages)
- ㆍ 발행정보
- 생화학분자생물학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
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Steroid의 핵구조(ring structure)가 $9{alpha}$-hydroxylation에 미치는 영향을 검토하기 위하여 $6{eta}$-methylandrost-4-ene-3, 17-dione, $6{alpha}$-methylandrost-4-ene-3, 17-dione, 19-norandrost-4-ene-3, 17-dione, androst-4-ene-3, 17-dione과 6, 19-oxidoandrost-4-ene-3, 17-dione 등 ${Delta}^4$-keto-system을 갖고 있는 steroid를 Nocardia opaca 배양액중에 첨가하여 주고 $9{alpha}$-hydroxylation 되는 과정을 살폈다. ${Delta}^4$-keto-system에 $6{eta}$-methyl group이 존재하면 현저히 hydroxylation의 속도가 느린 것을 발견하였다.
Rationalizing of conformational or structural modification effect on $9{alpha}$-hydroxylation of 17-ketosteroids which have ${Delta}^4$-3-keto-system was attempted. When $6{eta}$-methylandrost-4-ene-3, 17-dione was incubated with Nocardia opaca. the rate of $9{alpha}$-hydroxylation was much slower than that of androst-4-ene-3, 17-dione. In contrast, $9{alpha}$-hydroxylation of $6{alpha}$-methylandrost-4-ene-3, 17-dione was faster than that of $6{eta}$-isomer. In addition to these two compounds, 19-norandrost-4-ene-3, 17-dione and 6.19-oxidoandrost-4-ene-3, 17-dione were also compared with androst-4-ene-3, 17-dione, and it was found that the rate of $9{alpha}$-hydroxylation and B-ring rupturing of androst-4-ene-3, 17-dione was fastest among tested steroids.