양송이(Agaricus campestris var. bisporus)의 액체배양을 위하여 TGY 배지(培地)에서의 배양조건을 보면 $27{sim}30^{circ}C$ 진탕배양에서 pH는 배양일수에 따라 증가하는 경향을 나타내었고, 배양(培養) 12일에는 균체량의 생산도 많았고 질소함량도 7%를 유지하였으며, 배지중 탄소원은 거의 소실되었다. 배양기의 질소원으로는 ${(NH_4)}_2HPO_4$에서 수율(收率)이 좋았고, yeast extract 를 첨가하였을 때 균사체량이 더 증가하였다. 탄소원으로서는 glucose 가 가장 좋았고 fructose, maltose, lactose, sucrose 는 거의 비슷하였으며 가용성 전분은 거의 자화(資化)하지 못하였다. 양송이 균사체(菌絲體)는 단백질 함량이 48%이고 유리(遊離)아미노산으로서는 arginine, histidine, isoleucine, leucine, lysine, phenylalanine, proline, tyrosine 의 8종이 검출되었으며 단백질에는 각종 필수(必須)아미노산 특히 lysine, threonine이 많이 포함되어 있으므로 고품질(高品質)의 단백질 식품으로서 가치가 있음을 보여주었다.
Conditions for submerged culture of Agaricus campestris var. bisporus and the chemical composition of its mycelium were investigated. In shaking culture with TGY basal medium at $27{sim}30^{circ}C$, pH tended to increase upon culture period, mycelial growth was the highest on 12th day, with relatively high nitrogen content of 7% and sugar in the medium disappeared almost at the end of culture period. As a nitrogen source, ammonium phosphate (dibasic) gave relatively high mycelial yield and the addition of yeast extract gave rise to better results. As a carbon source, glucose was the best, fructose, maltose, lactose and sucrose gave the same results, and soluble starch was utilized slightly. Mushroom mycelium contained 48% of protein, 8 free amino acids including arginine, histidine, lysine, isoleucine, leucine, phenylalanine, proline, tyrosine and its protein consisted of most essential amino acids, with relatively high contents of lysine and threonine. Therefore, mushroom mycelium deserves to be a high quality protein food.
