Saponin was distributed in all tissues of Phytolacca americana and P. esculenta. Phytolaccoside E was a major saponin, but the callus tissues induced from stems of P. americana contained phytolaccoside B as a major component. Saponin contents in leaves and stems were almost similar to those in roots. Two unknown compounds, $C_{32}H_{50}O_{4}$ (triterpenoid) and $C_{18}H_{22}O_{5}{cdot}H_2O$ (polyphenol) were isolated from seeds of both plant.