인삼 성분을 메탄올로써 추출하고 이것이 쥐에 있어서 당질의 산화와 당의 생성 즉 glycogenesis 및 glyconeogenesis 그리고 당 신생반응의 첫 단계 반응을 조절하는 효소라고 믿고 있는 간의 phosphoenolpyruvate carboxylkinase의 활성에 어떠한 영향을 미칠것인가를 조사하여 보았다. 인삼 추출물을 쥐 체중 100g당 5mg이 되도록 생리적 식염수 0.5ml에 녹여서 1일 1회 4일간 복강내로 미리 투여하였으며 대조군에는 같은 양의 식염수만 주입하였다. 이것을 다시 계속 섭식시킨 군과 24시간 굶긴 군으로 나누어서 인삼 추출물을 투여한 1시간뒤에 glucose-$^{14}C$ 또는 amino acid mixed-$^{14}C$를 주입하여 호기중애 배출되는 $^{14}CO_2$ 양을 검출하고 또 3시간 후에 간속의 glycogen과 phospho-enolpyruvate caboxykinase에 대해서 검토하였다. 이 실험 결과 인삼은 glucose의 산화를 현저히 증가시켰으며 이 효과는 기아상태에서 더욱 촉진적으로 작용하였다. 그러나 인삼은 간에서의 glycogenesis나 glyconeogenesis에 대해서는 효능이 그리 없는것 같았다. 즉 인삼투여군은 대조군에 비하여 간의 glycogen농도나 $^{14}C$ 화합물의 glycogen에의 편입속도가 다같이 별 차이를 나타내지 않았다. 그리고 간의 phosphoenolpyruvate carboxykinase의 활성에도 인삼의 별 영향을 미치지 않았다.
Cultivated dry ginseng root was pulverized and extracted with methanol. The extractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200~220g were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5mg per 100g body weight of the ginseng extract in 0.5ml of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 uc of glucose-$^{14}C$ with carrier 100mg glucose per 100g of body weight or $4;{mu}c$ of amino acid mixed-$^{14}C$ with carrier 20mg L-alanine per 100g of body weight were injected peritoneally for the correspond group and control. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase, To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately after the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-$^{14}C$ or amino acid mixed-$^{14}C$ in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the adminisration of ginseng both the time of feeding and fasting.
