We studied on the separation of $alpha$- and $eta$-chain globins, the conditions of paper electrophoresis and mobility of globins in paper electrophoresis to determine their chain anomaly. Acidic-acetone mothod of Wilson and Smith was found to be more effecient for separating $alpha$- and $eta$chain globions than trichloroacetic acid method of Hayashi. The conditions of paper electrophoresis were current of 20v/cm and 0.6mA/cm for three hours at room temperature. In paper electroporesis, the mobility of $alpha$- -chain globin towards the cathode was greater than that of $eta$-chain globin. The results showed tha tchain anomaly of $alpha$- and $eta$-chain globins can be determined by the method of paper electrophoresis.