$varepsilon$-fructoselysine이 백서 소장내 disaccharidase에 미치는 영양을 in vivo 및 in vtro계에서 연구하였다. $varepsilon$-fructoselysine은 ($37^{circ}C{pm}0.5$, R.H. $70{pm}3%$) 10g의 milk casein과 1.6g의 포도당을 비교적 완화한 조건하에서 얻은 갈변 casein으로부터 분리되어 합성 표준품과(Rf 0.23) 비교하여 그 순도를 확인 하였다. 5% $varepsilon$-fructoselysine 식이를 투여하여 시험한 군이 백서의 Disaccharidase의 활성 시험에서 현저하게 감소되었다. in vitro에서의$varepsilon$-fructoselysine의 maltase 활성에 관한 길항작용의 형식은 경쟁적 길항효과를 나타내었다($K_i=3.6;mol$). 갈변 casein식이를 성장 백서에 투여 사육한 실험에서 세시간후에 나타난 L-lysine과 $varepsilon$-fructoselysine은 일곱시간후에는 L-lysine이 사라지고 $varepsilon$-fructoselysine은 소장내강에서 흡수되지 못하고 가수분해도 않되어 축적되는 물질로 상기한 억제효과에 큰 몫을 준다.
Effect of $varepsilon$-fructoselysine on the activity of disaccharidase in the small intestine of male albino rats in vivo and in vitro systems was investigated. $varepsilon$-fructoselysine (Amadori compound, 1-deoxy-1-(-N-L-lysino)-D-fructose) was prepared from. browned casein which was formed 10g of milk casein and 1.6g of glucose under relatively mild conditions ($37^{circ}C{pm}0.5$, R.H. $70{pm}3%$), and its purity was confirmed with the synthetic standard (Rf value, 0.23). Disaccharidase activities in the rats fed 5% $varepsilon$-fructoselysine diet were proved to be significantly reduced. Using a model system ($varepsilon$-fructoselysine), the in vitro study on the mode of inhibitory effect on maltase activity revealed that the comopund showed a competitive inhibitory effect (Ki=3.6mo1). When browned casein (condition mentioned above) was ingested by growing rats, $varepsilon$-fructo selysine which was found after 3 hr, was remained from the intestinal TCA-soluble fraction, but L-lysine disappeared after 7 hr from this result, $varepsilon$-fructoselysine which remained as an absorption-delayed material in the small intestinal lumen is responsible for its inhibitory effect.
